Cellic htec3

Manufactured by Novozymes

Sourced in United States

Cellic® HTec3 is a laboratory equipment product produced by Novozymes. It is a cellulase enzyme preparation designed for hydrolysis of cellulosic materials.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

HTec3 (8 citations)

6 protocols using cellic htec3

Enzymatic Saccharification of Rice Straw

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the chemicals used in this study were procured from Sigma Aldrich and Merck, India.
¹³C gluconic acid (99 atom % ¹³C) were purchased from Omicron Biochemicals, USA, and LC/MS grade water was obtained from Biosolve Chimie, SARL, France. Commercial enzymes Cellulase from T. reesei ATCC 26921 (Celluclast 1.5L) and β-glucosidase (Novozymes 188) were procured from Sigma, Aldrich India. Cellic^® Ctec2 and Cellic^® HTec3 were a kind gift from Novozymes, Mumbai, India. Avicel^® PH 101 and dilute acid-treated rice (Oryza sativa) straw were used as substrates for enzymatic saccharification studies. Rice (Oryza sativa) straw was procured from the local market at Mathura (27.28° N, 77.41° E) in Uttar Pradesh (North, India) air-dried, ground to the particle size of ~ 10 mm using a knife mill ,and stored in an air tight container for further use. All the experiments were conducted from the single stock of harvested rice straw.

Dixit P., Basu B., Puri M., Tuli D.K., Mathur A.S, & Barrow C.J. (2019). A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains. Biotechnology for Biofuels, 12, 185.

+ Open protocol

+ Expand

Biomass Pretreatment and Enzyme Utilization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hemp hurd and packaging materials were donated by Ecovative Design LLC (Green Island, NY, USA) for evaluation. Both materials were sundried for 24 h and knife-milled with a 2 mm screen (Thomas-Wiley model 4, Swedesboro, NJ, USA). For biomass pretreatment, cholinium lysinate [Ch][Lys] was obtained from Proionic (Grambach, Styria). Commercial cellulase (Cellic CTec3) and hemicellulase (Cellic HTec3) were provided by Novozymes (Franklinton, NC, USA). Sulfuric acid (72% and ACS reagent, >99%), glucose (>99.5%), xylose (>99%), and arabinose (>98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Choi J., Rodriguez A., Simmons B.A, & Gladden J.M. (2023). Valorization of Hemp-Based Packaging Waste with One-Pot Ionic Liquid Technology. Molecules, 28(3), 1427.

+ Open protocol

+ Expand

Switchgrass Conversion Residue Bioethanol Production

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Switchgrass used to generate conversion residue was treated by ammonia fiber expansion and enzymatically treated with Cellic CTec3 and Cellic HTec3 (Novozymes) to digest cellulose and hemicellulose (to produce glucose and xylose, primarily) (38 (link)). Hydrolysate was fermented with Saccharomyces cerevisiae Y128, a strain with improved xylose utilization (39 (link)). Fermentation media were distilled to remove ethanol (4 (link)).

Scarborough M.J., Lawson C.E., Hamilton J.J., Donohue T.J, & Noguera D.R. (2018). Metatranscriptomic and Thermodynamic Insights into Medium-Chain Fatty Acid Production Using an Anaerobic Microbiome. mSystems, 3(6), e00221-18.

+ Open protocol

+ Expand

Preparation of Authentic AFEX Corn Stover Hydrolysate

Check if the same lab product or an alternative is used in the 5 most similar protocols

Preparation for authentic AFEX Corn stover hydrolysate
Corn stover with glucan and xylan contents of 38.0% and 23.5%, respectively, was AFEX pretreated as previously described (Balan et al. 2009 ). AFEX pretreatment conditions applied were 1:1 ammonia to biomass ratio, 0.6 g water per g dry biomass, 140 °C and 30 min. AFEX pretreated corn stover has the same composition as the untreated one. AFEX pretreated corn stover was enzymatically hydrolyzed at 6.0 wt.% glucan loading in an Applikon Ez-Control 5L bioreactor with a total mixture of 4000 g. Enzymes used for hydrolysis included Cellic Ctec3 (Novozymes, Franklinton, NC): 12 mg protein/g glucan (0.022 ml Ctec3/g dry biomass) and Cellic Htec3 (Novozymes, Franklinton, NC): 9 mg protein/g glucan (0.021 ml Htec3/g dry biomass). The protein concentrations in Ctec3 (210.6 mg/ml) and Htec3 (164.6 mg/ml) were estimated by Dairyone (Ithaca, NY) using Kjeldahl method. Enzymatic hydrolysis was conducted at 50°C and pH 4.8 for 96 h. The hydrolysate was then centrifuged and unhydrolyzed solids were removed. The liquid hydrolysate was immediately sterile filtered after centrifugation and stored at 4 °C.

Sitepu I.R., Jin M., Fernandez J.E., da Costa Sousa L., Balan V, & Boundy-Mills K.L. (2014). Identification of oleaginous yeast strains able to accumulate high intracellular lipids when cultivated in alkaline pretreated corn stover. Applied microbiology and biotechnology, 98(17), 7645-7657.

+ Open protocol

+ Expand

Sorghum biomass pretreatment and enzymatic hydrolysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

All materials were used as supplied unless otherwise noted. Water was deionized, with specific resistivity of 18 MΩ·cm at 25 °C, from Purelab Flex (ELGA, Woodridge, IL, USA). Choline hydroxide (45% in methanol), acetic acid (>99.7%), sodium hydroxide pellets (≥97%), methanol, sodium azide, and sulfuric acid (98%) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ethanol (200 proof) was purchased from Decon Labs, Inc. (King of Prussia, PA, USA). sulfuric acid (72%) was procured from the RICCA chemical company (Arlington, TX, USA). J. T. Baker, Inc. (Phillipsburg, NJ, USA) supplied hydrochloric acid and sodium citrate dihydrate, while citric acid monohydrate (≥99.99%) was obtained from Merck (Kenilworth, NJ, USA).
Analytical standard grade glucose and xylose were also obtained from Sigma-Aldrich (St. Louis, MO, USA) and used for calibration.
Sorghum (Sorghum bicolor, donated by Idaho National Labs, Idaho Falls, ID, USA) was dried for 24 h in a 40 °C oven. Subsequently, it was knife-milled with a 2 mm screen (Thomas-Wiley Model 4, Swedesboro, NJ, USA). The resulting biomass was then placed in a leak-proof bag, and stored in a dry cool place (4 °C room during the period of use).
Commercial cellulase (Cellic^® CTec3) and hemicellulase (Cellic^® HTec3) mixtures were provided by Novozymes, North America (Franklinton, N, USAC).

Choudhary H., Pidatala V.R., Mohan M., Simmons B.A., Gladden J.M, & Singh S. (2022). Renewable Schiff-Base Ionic Liquids for Lignocellulosic Biomass Pretreatment. Molecules, 27(19), 6278.

+ Open protocol

+ Expand

Sorghum Pretreatment and Enzyme Hydrolysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sorghum was provided by Idaho National laboratory and ground using a Wiley mill through a 2mm screen and separated by a vibratory sieve system (Endecotts, Ponte Vedra, FL, USA). The commercial enzyme products Cellic® CTec3 and Cellic® HTec3 were gifts from Novozymes, North America (Franklinton, NC, USA). Cholinium aspartate was purchased from IoLiTec (Heilbronn, Germany).

Park M.R., Chen Y., Thompson M., Benites V.T., Fong B., Petzold C.J., Baidoo E.E.K., Gladden J.M., Adams P.D., Keasling J.D., Simmons B.A, & Singer S.W. (2020). Response of Pseudomonas putida to Complex, Aromatic-Rich Fractions from Biomass. ChemSusChem, 13(17).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!