Tsk g3000swxl

Manufactured by Tosoh

Sourced in Japan

The TSK G3000SWXL is a size exclusion chromatography column used for the analysis of macromolecules such as proteins, polymers, and other high molecular weight compounds. It is designed to separate molecules based on their size and molecular weight. The column features a silica-based stationary phase and is suitable for aqueous and organic mobile phases.

Automatically generated - may contain errors

Lab products found in correlation

Tsk g4000 swxl, by Tosoh (3 mentions) Gel filtration standard, by Bio-Rad (2 mentions) Seeblue plus2, by Thermo Fisher Scientific (1 mentions) Agilent 1200 series hplc system, by Agilent Technologies (1 mentions) Lc 10ai pump, by Shimadzu (1 mentions) Rid 10a refractive index detector, by Shimadzu (1 mentions) Cbm 20a controller, by Shimadzu (1 mentions) V 650 spectrophotometer, by Jasco (1 mentions) Anhydrous citric acid, by Merck Group (1 mentions) N ethyl n 3 dimethylaminopropyl carbodiimide edc, by Merck Group (1 mentions) Sephadex g 15, by GE Healthcare (1 mentions) Hochest, by Merck Group (1 mentions) Pd 10 desalting columns containing sephadex g 25 resin, by GE Healthcare (1 mentions) Tsk g2000swxl, by Tosoh (1 mentions) N hydroxysuccinimide nhs, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Merck Group (1 mentions) Dimethylformamide dmf, by Merck Group (1 mentions) Optilab t rex, by Wyatt Technology (1 mentions) Fp 6500 spectrofluorometer, by Jasco (1 mentions)

8 protocols using tsk g3000swxl

Protein Expression and Purification Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

A standard method SDS-PAGE (NuPAGE) used for detecting protein expression and purifications, and a SeeBlue Plus2 prestained standard (from Invitrogen) was run in parallel with samples for molecular weight determination. Following electrophoresis, the gel was washed then stained using SimplyBlue SafeStain, and gel images were captured using a Bio-Rad Molecular Imager Gel Doc XR⁺ System and analysed by Image Lab ChemiDoc MP Software.
Protein molecular weights in their native state (in full homogeneity) were investigated using analytical size exclusion-HPLC with a TSK column (TSK G3000SWXL, Tosoh Bioscience) and Agilent 1200 Series HPLC System (Agilent Technologies). Protein samples run in 0.1 M Phosphate (pH 7.8), 0.2 M NaCl in a maximum of 100 μL sample/injection (equivalent to 1 mg/mL) were analysed at a flow rate of 0.8 mL/min (≤70 bar), at 25 °C. A 10 μL BioRad Gel filtration standard (Bio-Rad Laboratories), was applied for determining apparent molecular weight of target proteins from a standard curve of proteins of known size.

Alsultan A.M., Chin D.Y., Howard C.B., de Bakker C.J., Jones M.L, & Mahler S.M. (2016). Beyond Antibodies: Development of a Novel Protein Scaffold Based on Human Chaperonin 10. Scientific Reports, 6, 37348.

+ Open protocol

+ Expand

Molecular Weight Determination of Heparin by HPLC-GPC

Check if the same lab product or an alternative is used in the 5 most similar protocols

The molecular weight was determined by HPLC-gel permeation chromatography (GPC) using USP heparin as standard. HPLC system is consisting of Shimadzu LC-10Ai pump, a Shimadzu CBM-20A controller and a Shimadzu RID-10A refractive index detector (Shimadzu, Kyoto, Japan). A guard column TSK SWXL 6 mm × 4 cm, 7 µL diameter was used to protect two analytical columns: TSK G4000 SWXL 7.8 mm × 30 cm, 8 µm in series with TSK G3000SWXL 7.8 mm × 30 cm, 5 µm (Tosoh Corporation, Tokyo, Japan). The mobile phase was 0.1 M ammonium acetate with 0.02% (w/v) sodium azide. Columns were maintained at 30 °C using an Eppendorf column heater (Eppendorf, Hamburg, Germany). The sample injection volume was 20 µL with concentration at 5 mg/mL and flow rate set at 0.6 mL/min.

Yu Y., Bruzdoski K., Kostousov V., Hensch L., Hui S.K., Siddiqui F., Farooqui A., Kouta A., Zhang F., Fareed J., Teruya J, & Linhardt R.J. (2020). Structural characterization of a clinically described heparin-like substance in plasma causing bleeding. Carbohydrate polymers, 244, 116443.

+ Open protocol

+ Expand

Quantifying ATP Binding to TDnaK Mutants

Check if the same lab product or an alternative is used in the 5 most similar protocols

TDnaK and its mutants (10 mg·ml⁻¹) were incubated with 3 mM ATP at 55 °C for 60 min. The mixture was centrifuged for 1 min at 21,000 g. An aliquot (100 μl) was loaded on the HPLC gel filtration column TSK G-3000SWXL (Tosoh) equilibrated with 50 mM MOPS-NaOH (pH 7.5), 150 mM KCl, and 5 mM MgCl₂ at room temperature. The eluted TDnaKs were concentrated to approximately 1.7 mg·ml⁻¹ by using a 30 K Amicon ultra device. The absorption spectra of these solutions were measured using a V-650 spectrophotometer (JASCO). From the difference spectrum between the spectrum of the same proteins with or without ATP incubation, the amounts of bound ATP were calculated using the extinction coefficient of ATP (ε_{259 nm} = 15,400 M⁻¹ cm⁻¹).

Hayashi S., Nakazaki Y., Kagii K., Imamura H, & Watanabe Y.H. (2017). Fusion protein analysis reveals the precise regulation between Hsp70 and Hsp100 during protein disaggregation. Scientific Reports, 7, 8648.

+ Open protocol

+ Expand

Carbodiimide-Mediated Synthesis of PEGylated Biomaterials

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dicyclohexyl carbodiimide (DCC) (Cat # 802954), Dimethylformamide (DMF) (Cat # 103053), Poly (ethylene glycol) diacid (Cat # 843912), and anhydrous citric acid (Cat # 818707) were purchased from Merck. PD‐10 desalting columns containing Sephadex G‐25 resin (Cat # 170851) and Sephadex G15 (Cat # 170020) were purchased from GE Healthcare. N‐Hydroxysuccinimide (NHS) (Cat # 130672), RPMI 1640 (Cat # R8758), XTT (Cat # X4626), Hochest (Cat # B1155), and N‐Ethyl‐N′‐(3‐dimethylaminopropyl) carbodiimide (EDC) (Cat # E1769) were purchased from Sigma Aldrich. A549, L929, and NFS60 cell lines, rats, and mice were obtained from Iranian Genetic Resources Center and Pasteur Institute of Iran, respectively. G‐CSF was purchased from Cinnagen. Fetal bovine serum (FBS) (Cat # 10270) was purchased from GIBCO Invitrogen. Filter papers (Cat # DP 150‐150) and microplates (Cat # 3635) were purchased from Albet and corning, respectively. TSK G2000SWXL (Cat # 0008540), TSK G3000 SWXL (Cat # 0008541), and Guard (Cat # 0008543) were purchased from Tosoh. Quantikine ELISA kit for determination of G‐CSF (Cat # DCS50) was purchased from R&D systems.

Mousavi Motlagh S.S., Seyedhamzeh M., Ahangari Cohan R., Shafiee Ardestani M., Vaziri B., Azadmanesh K., Saberi S, & Masoumi V. (2021). Novel G‐CSF conjugated anionic globular dendrimer: Preparation and biological activity assessment. Pharmacology Research & Perspectives, 9(4), e00826.

+ Open protocol

+ Expand

Precursor Binding Affinities of SecB Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Each of the cross-linked SecB heterotetramers was shown to bind precursor galactose-binding protein by size exclusion chromatography using a TSKG3000SW_XL (Tosoh Corp., Japan) column as described below except the buffer contained 1 mM ethylene glycol tetraacetic acid to keep the precursor in an unfolded state. The relative affinities for the precursors were not quantitatively determined and might vary as much as four-fold.
Each hybrid except SecBDb, which has the lowest affinity for SecA (at least 10-fold lower than does wild-type SecB), was assayed for in vitro translocation using inverted membrane vesicles and precursor galactose-binding protein as described in Mao et al. [22 (link)]. Species that populate A2:B4 complexes showed approximately 1.7-fold higher activity as compared to those that form A1:B4 complexes as expected [1 (link)] (see Supplemental Data).

Suo Y., Hardy S.J, & Randall L.L. (2014). The Basis of Asymmetry in the SecA-SecB Complex. Journal of molecular biology, 427(4), 887-900.

+ Open protocol

+ Expand

Analytical Size-Exclusion HPLC of Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Purity and apparent molecular weight (MW) of HlaWt and HlaPSGS proteins were determined by an analytical size-exclusion high-pressure liquid chromatograph (HPLC) TSK G3000SWxl (7.8 by 300 mm; Tosoh Bioscience Corporation) with isocratic elution in PBS at a flow rate of 0.5 ml/min. UV absorbance was monitored at 280 nm. For molecular mass determination, a gel filtration standard (670, 158, 44, 17, and 1.35 kDa; Bio-Rad) was applied.

Fiaschi L., Di Palo B., Scarselli M., Pozzi C., Tomaszewski K., Galletti B., Nardi-Dei V., Arcidiacono L., Mishra R.P., Mori E., Pallaoro M., Falugi F., Torre A., Fontana M.R., Soriani M., Bubeck Wardenburg J., Grandi G., Rappuoli R., Ferlenghi I, & Bagnoli F. (2016). Auto-Assembling Detoxified Staphylococcus aureus Alpha-Hemolysin Mimicking the Wild-Type Cytolytic Toxin. Clinical and Vaccine Immunology : CVI, 23(6), 442-450.

+ Open protocol

+ Expand

Heparin Hydrolysates Characterization by SEC-MALS

Check if the same lab product or an alternative is used in the 5 most similar protocols

The heparin hydrolysates were analysed at a concentration of 5 mg/mL. Each analytical sample was filtered through a 0.45 µm regenerated cellulose (RC) syringe filter (17 mm, Target2™, Thermo Fisher Scientific, Paisley, UK), prior to injection (100 µL). Monodisperse bovine serum albumin (BSA) standards were prepared in identical solvents to those of experimental samples, at a concentration of 2 mg/mL and used for normalization of the system.
Hydrolysates of heparin were separated on TSK G4000 SWXL and TSK G3000 SWXL columns (Tosoh Bioscience, Tokyo, Japan), protected by similarly packed guard column and eluted with a 0.1 M NH4OAc + 0.05% (w/v) NaN3 mobile phase at a flow rate of 0.6 mL/min and temperature of 30 °C. Samples were detected using MALS (DAWN 8+) and refractive index (Optilab T-REX) detectors (Wyatt Technology Corporation, Santa Barbara, USA). Data for molecular weight determinations were analyzed using Astra software v. 6.1.5 (Wyatt Technology Corporation, Santa Barbara, USA). Refractive index increments (dn/dc) of 0.131 mL/g for heparin 42 (link) and 0.185 mL/g for BSA 43 were used respectively in data processing.

Kozlowski A.M., Yates E.A., Roubroeks J.P., Tømmeraas K., Smith A.M, & Morris G.A. (2021). Hydrolytic Degradation of Heparin in Acidic Environments: Nuclear Magnetic Resonance Reveals Details of Selective Desulfation. ACS applied materials & interfaces, 13(4).

+ Open protocol

+ Expand

Pullulan Characterization by GPC-RI

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gel permeation chromatography (GPC) was performed using two columns in series: TSK G3000SWXL and TSK G4000SWXL (7.8 × 300 mm, Tosoh) operated on a HPLC system equipped with a refractive index (RI) detector and isocratically eluted using ultrafiltered water at room temperature, at a flow rate of 0.6 mL/min. Molecular weight (Mw) and polydispersity (Mw/Mn) of native and derivatized pullulan were determined on a calibration curve obtained with pullulan standards (270, 150, 80, 50, 25, 12 kDa). μL of the polymer solutions and the samples were maintained under gentle shaking at room temperature overnight, protected from the light. The solutions were incubated at 37 °C for 15 min and fluorescence excitation spectra were recorded in the 300 to 360 nm wavelength range with an emission wavelength (λem) set at 390 nm using a Jasco FP-6500 Spectrofluorometer.
The I(λex 339 nm)/I(λex 334 nm) intensity ratios were plotted as a function of the logarithm of the polymer concentration (logC). The CMC was determined from the intersection point of the resulting two curves.

Bonzi G., Salmaso S., Scomparin A., Eldar-Boock A., Satchi-Fainaro R, & Caliceti P. (2015). Novel pullulan bioconjugate for selective breast cancer bone metastases treatment. Bioconjugate chemistry, 26(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!