Common solvents were purchased from Sigma-Aldrich (St. Louis, MO). Tissue-Tek O.C.T. compound (Sakura 4583, Cat # 25608-930) and 100% ethanol (Decon Labs, Cat # 71006-012) were purchased from VWR. Toluidine blue O (Cat # 198161-25G) was purchased from Sigma-Aldrich. Anti-insulin antibody clone K36aC10 (Cat # I2018, 1:1000) was purchased from Sigma-Aldrich. PPS Silent Surfactant was purchased from Expedeon (San Diego, California, Cat # 21011). Histostain Plus Broadspectrum AEC kit was purchased from Invitrogen (Cat # 85-8943).
Histostain plus broad spectrum aec kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Histostain-Plus Broad Spectrum (AEC) Kit is a laboratory equipment product designed for immunohistochemistry applications. The kit provides reagents and protocols for the detection of target antigens in tissue sections or cellular preparations.
Automatically generated - may contain errors
Lab products found in correlation
Anti insulin, by Santa Cruz Biotechnology (2 mentions)
Anti glucagon, by Santa Cruz Biotechnology (2 mentions)
Clear mount mounting medium, by Electron Microscopy Sciences (2 mentions)
Toluidine blue o, by Merck Group (1 mentions)
Ethanol, by Decon Labs (1 mentions)
Common solvents, by Merck Group (1 mentions)
Pps silent surfactant, by Abcam (1 mentions)
Dm2500, by Leica (1 mentions)
Digital camera dfc 425 c, by Leica (1 mentions)
Eclipse 80i light microscope, by Nikon (1 mentions)
Eclipse 80i, by Nikon (1 mentions)
4 protocols using histostain plus broad spectrum aec kit
1
Immunohistochemical Analysis of Insulin
2
Histological Analysis of Mouse Liver and Pancreas
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Small pieces of liver tissues and pancreas were fixed with formalin (200 g/kg) neutral buffered solution and embedded in paraffin. Sections (8 µm) were cut and stained with hematoxylin and eosin. For microscopic examination, a microscope (Leica, DM2500) was used, and the images were taken using a Leica Digital camera (DFC-425-C) at 10 (ocular) × 10 (object lens) magnification. Each presented image is typical and representative of seven mice.
An immunohistochemical (IHC) staining for insulin (brown) and glucagon (green) in the pancreatic islets of mice was assessed according to a previous study [59 (link)], and briefly, anti-insulin (1 : 100, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA, no. sc-9168) or anti-glucagon (1 : 200, Santa Cruz Biotechnology, no. sc-13091) primary antibodies were used. Staining was developed using Histostain-Plus Broad Spectrum (AEC) Kit (Invitrogen, Frederick, MD, USA, no. 859943). The IHC procedure was conducted according to manufacturer instructions, and image were taken at 400 magnification.
An immunohistochemical (IHC) staining for insulin (brown) and glucagon (green) in the pancreatic islets of mice was assessed according to a previous study [59 (link)], and briefly, anti-insulin (1 : 100, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA, no. sc-9168) or anti-glucagon (1 : 200, Santa Cruz Biotechnology, no. sc-13091) primary antibodies were used. Staining was developed using Histostain-Plus Broad Spectrum (AEC) Kit (Invitrogen, Frederick, MD, USA, no. 859943). The IHC procedure was conducted according to manufacturer instructions, and image were taken at 400 magnification.
3
Quantification of Pancreatic Insulin Content
4
Immunohistochemical Analysis of Pancreatic Cells
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Immunohistochemistry procedures have been described previously [18 (link)]. Anti-insulin (1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, #sc9168), anti-glucagon (1:200, Santa Cruz Biotechnology, #sc13091), or anti-somatostatin (1:200, Abcam, #ab15365) primary antibodies were used for pancreas immunostaining. Staining was developed using Histostain-Plus Broad Spectrum (AEC) Kit (Invitrogen, Frederick, MD). Slides were counterstained with hematoxylin to identify cell nuclei.
After staining, slides were rinsed in deionized water and placed on coverslips in Clear Mount mounting medium (Electron Microscopy Sciences, Hatfield, PA). The specificity of immunoreactivity was confirmed by omitting the primary antibody from some sections. The staining was observed using a light microscope Nikon Eclipse 80i (Nikon Instruments, Melville, NY). Images were analyzed using Adobe Photoshop CZ4 extended software.
After staining, slides were rinsed in deionized water and placed on coverslips in Clear Mount mounting medium (Electron Microscopy Sciences, Hatfield, PA). The specificity of immunoreactivity was confirmed by omitting the primary antibody from some sections. The staining was observed using a light microscope Nikon Eclipse 80i (Nikon Instruments, Melville, NY). Images were analyzed using Adobe Photoshop CZ4 extended software.
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