Rabbit anti sting

According to our previous report,⁶³ (link) after being deeply anesthetized with pentobarbital sodium, rats were transcardially perfused with 150 mL of 1× PBS (4°C) followed by 150 mL of 4% paraformaldehyde (4°C). Lumbar enlargement of the spinal cord was collected, postfixed in 4% paraformaldehyde for 48 h at 4°C, and subsequently dehydrated with 30% sucrose for 3 days at 4°C. Subsequently, the spinal cord was cut transversely into slices with a thickness of 30 μm. The sections were permeabilized with 0.3% Triton X-100 for 10 min and blocked with 10% sheep or donkey serum for 2 h at 24°C. Sections were incubated with the following primary antibodies for 48 h at 4°C: goat-anti-Iba1 (1:200, Abcam, Cambridge, UK), mouse anti-GFAP (1:500, Cell Signaling Technology), mouse-anti-NeuN (1:500, Abcam), rabbit-anti-pTAOK2 (1:100, GeneTex), rabbit-anti-cGAS (1:100, Cell Signaling Technology), and rabbit-anti-STING (1:100, Cell Signaling Technology). After washing with 1× PBS, the sections were incubated with fluorescent secondary antibodies in the dark for 2 h at 24°C. Finally, the sections were examined under a fluorescence microscope (FV3000; Olympus, Japan).