Sinusitis was induced using a transnasal endoscopic technique as previously described by Cho et al. (2018) (link). In brief, sterile nasal packing material (Merocel®, Medtronic, Auckland, New Zealand) was placed in the left middle meatus (unilateral) under endoscopic guidance to achieve complete obstruction of this sinus ostium. All procedures were carried out by an experienced otorhinolaryngology surgeon with the assistance of a veterinary anaesthetist and an animal welfare officer to ensure the best possible care for the rabbits during surgery. Our pilot study indicated that two weeks of sinus obstruction was insufficient to achieve significant and persistent sinus inflammation (data not shown). Accordingly, the blockage period was extended to four weeks. After the removal of Merocel®, all animals were observed for a further ten weeks, i.e., up to 14 weeks from the time point of obstruction. A timeline of the study is illustrated in Figure 1
Merocel
Manufactured by Medtronic
Sourced in United States
Merocel is a surgical sponge material used in various medical procedures. It is a highly absorbent and durable foam-based product designed to provide controlled absorption and retention capabilities. Merocel is suitable for use in various medical and surgical applications where absorbent materials are required. Its core function is to serve as an effective and reliable absorbent material in healthcare settings.
Automatically generated - may contain errors
Lab products found in correlation
Modular compact rheometer mcr 102, by Anton Paar (1 mentions)
Tisseel, by Baxter (1 mentions)
Glycidyl methacrylate gma, by Merck Group (1 mentions)
Quanti it pico green dsdna assay kit, by Thermo Fisher Scientific (1 mentions)
Deuterium oxide, by Merck Group (1 mentions)
Balanced salt solution, by Alcon (1 mentions)
Floseal, by Baxter (1 mentions)
Coblator, by Smith & Nephew (1 mentions)
Nasopore, by Stryker (1 mentions)
24 protocols using merocel
1
Rabbit Model of Chronic Sinusitis
2
Atresiaplasty Surgical Procedure for Middle Ear Reconstruction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Atresiaplasty was performed using an anterior approach, as described in previous studies.1 , 2 , 7 The mastoid was briefly exposed after a retroauricular skin incision. The epitympanum was entered by drilling the bone between the linea temporalis superiorly and the glenoid fossa anteriorly, following the tegmen. After the malleus–incus complex was identified, the atretic plate was removed, during which a diamond burr at low speed was used to avoid damage to the surrounding structures. When a structure, presumably, the CTN came out, precautions were taken to preserve it as much as possible. A new canal was created to center the ossicles in the middle of the canal. The integrity of the ossicular chain was checked, and disconnected ossiculoplasty was performed using either partial or total ossicular prosthesis. The temporalis fascia was placed over the malleus–incus complex, and a thin split‐thickness skin graft was placed on the fascia, creating a new canal. A silicone button was inserted into the external canal over the new tympanic membrane, and meatoplasty was performed after the elevation of the anterior‐based concha flap. The concha flap was secured to the periosteum, and a split‐thickness skin graft was sutured to the metal skin. The external canal and meatus were packed with Merocel® (Medtronic XOMED, Jacksonville, FL, USA) wicks.
3
Soy Protein-Chitin Nasal Pack Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Soy protein (SP), PROFAM 974, was supplied by ADM Protein Specialties Division (The Netherlands). The amino acid analysis as well as XPS and FTIR studies were performed in a previous work [15 (link)].
Chitin (CH) was extracted from fresh squid pens (Loligo sp.), which were treated with NaOH (1 M) at room temperature under continuous stirring for 24 h; afterwards, the solid fraction (CH) was washed with distilled water until neutral pH. Finally, CH was freeze-dried and milled to obtain the powder. Soy protein and chitin batches were those used and characterized in a previous work [14 (link)].
Glycerol, with a purity ≥99.5%, was supplied by Panreac (Barcelona, Spain). This polyol was employed as a plasticizer in order to disrupt intramolecular interactions among protein chains and facilitate intermolecular interactions between soy protein and chitin.
The properties of our SP-CH was compared with a standard gauze (Medicomp® 5 × 5 cm, Hartmann bv, Nijmegen, The Netherlands) and with Merocel® (Medtronic Xomed, Jacksonville, Fla.). This commercial nasal pack, which is composed of hydroxylated polyvinyl acetate, and presented as a compressed and dehydrated sponge, is the primary nonabsorbable nasal pack in several emergency departments around the world [[24] , [25] (link), [26] , [27] ].
Chitin (CH) was extracted from fresh squid pens (Loligo sp.), which were treated with NaOH (1 M) at room temperature under continuous stirring for 24 h; afterwards, the solid fraction (CH) was washed with distilled water until neutral pH. Finally, CH was freeze-dried and milled to obtain the powder. Soy protein and chitin batches were those used and characterized in a previous work [14 (link)].
Glycerol, with a purity ≥99.5%, was supplied by Panreac (Barcelona, Spain). This polyol was employed as a plasticizer in order to disrupt intramolecular interactions among protein chains and facilitate intermolecular interactions between soy protein and chitin.
The properties of our SP-CH was compared with a standard gauze (Medicomp® 5 × 5 cm, Hartmann bv, Nijmegen, The Netherlands) and with Merocel® (Medtronic Xomed, Jacksonville, Fla.). This commercial nasal pack, which is composed of hydroxylated polyvinyl acetate, and presented as a compressed and dehydrated sponge, is the primary nonabsorbable nasal pack in several emergency departments around the world [[24] , [25] (link), [26] , [27] ].
4
Transnasal Endoscopic Approach with Modified Trans-septal Technique
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Although the endoscopic transnasal approach is regarded as a minimally invasive technique, the damage of the adjacent sinonasal structures to obtain a sufficient surgical corridor is inevitable. In our experience, patients who underwent treatment with the trans-septal approach consistently had a relatively clear nasal cavity than those who underwent treatment with the transnasal approach (19) . Thus, in our medical center, all ETA procedures were performed by one rhinologist (S.D.H), and one of two neurosurgeons (D.H.N and D.S.K) using modified trans-septal approaches with a 4-mm, 0-degree, 30-degree, or 45-degree endoscope (20) .
The nasal cavity was decongested with 1:10,000 epinephrinesoaked cotton pledgets and the nasal septum was infiltrated with a lidocaine-containing epinephrine (1:100,000) solution. A hemitransfixion incision was performed in the left nasal septal performed using intradural and extradural multilayered fascia lata. This was subsequently covered with an NSF. After surgery, the anterior nasal packing was maintained for 3-7 days with Merocel (Medtronic Inc., Minneapolis, MN, USA).
The nasal cavity was decongested with 1:10,000 epinephrinesoaked cotton pledgets and the nasal septum was infiltrated with a lidocaine-containing epinephrine (1:100,000) solution. A hemitransfixion incision was performed in the left nasal septal performed using intradural and extradural multilayered fascia lata. This was subsequently covered with an NSF. After surgery, the anterior nasal packing was maintained for 3-7 days with Merocel (Medtronic Inc., Minneapolis, MN, USA).
5
EM Tracking Device for Nasal Procedure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EM tracking device used in this study is a waterproof, 5-mm-diameter, 2-cm-long, rod-type, 3D coil array for position detection within the field created by the EM emitter. It is contained in various instruments and trackers of the AxiEM navigation system (Medtronic USA). This device was manually removed from the housing of the wing-type ST and plugged into the conduit of a standard nasal dressing (Merocel, Medtronic USA) after removal of its silicone airway tube until close to the distal end (Fig. 1). With the aid of a short nasal speculum, this "nasal tracker" (NT) is inserted through the wider of the two nostrils into the posterior osseous part of the inferior nasal meatus. The cable is secured to the philtrum via a strain relief loop and surgical tape to prevent inadvertent malpositioning or removal by drag on its cable during a procedure (Fig. 2).
6
Endoscopic Transseptal Pituitary Surgery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After administration of general anesthesia, the patient was elevated by 15° using an operative pillow. The patient’s head was then fixed in a head holder with 10°–20° rotation towards the operator. A standard microscopic uninostril transseptal technique was performed during the surgery. After binostril preparation and draping, a hemitransfixion incision was made in the right nasal septal mucosa. Then, a subperiosteal dissection was performed between the septal bone and mucosa with a nasal speculum to expose the anterior wall of the sphenoidal sinus. We opened the sphenoidal sinus widely and resected the sphenoidal spectrum and sinus mucosa to reach the sellar floor. An X-shaped incision was used to incise the dura, and the tumor was resected using various angled curettes, pituitary rongeurs, and suction. Following the resection of the tumor and hemostasis, we carefully inspected the sellar cavity and used multilayer techniques to reconstruct the sellar floor, which included fat (if necessary), Dural Graft Matrix (DuraGen, INTEGRA, USA), artificial dura (Aesceulap, USA), and a Porcine Fibrin Sealant Kit (BeiXiu, China). Finally, we inspected the whole surgical cavity, repositioned the right septal mucosa flap, and inserted two nasal tamponades (MEROCEL, Medtronic, USA) into each nasal cavity.
7
Ambulatory Sinonasal Procedures in Day-Case Surgery
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All patients were treated under general anesthesia in dedicated operating rooms for day-case surgery. All procedures were completed before 2:00 pm. Based on the International Classification of Diseases, Tenth Revision, four groupings of sinonasal procedures were assembled: endoscopic sinus surgery (ESS), rhinoplastic surgery, septoplasty and/or turbinate surgery and closed reduction of nasal bone fracture. For each patient, the length of procedure was reported. Nasal packing was used at the discretion of the individual surgeon. Algosteril™ (Brothier Inc, Nanterre, France) or Merocel™ (Medtronic Inc, Dublin, Ireland) were used for ESS. Merocel™ was exclusively used during the other 3 procedures. Postoperatively, patients were monitored in the post-anesthesia care unit and then the ambulatory surgical unit. Patients were discharged from the ambulatory surgical unit when they met appropriate discharge criteria according to the post-anesthesia discharge scoring system. This score is based upon the recovery of vital signs without active bleeding and the control of pain, nausea and vomiting (14) . Distance traveled between home and the ambulatory surgical unit was reported.
8
Post-Operative Nasal Packing and Monitoring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
None of patients underwent surgery of septum and the turbinates, while all patients applied nasal packing with Merocel [Medtronic Xomed, Jacksonville, Fla.] to prevent postoperative bleeding, and all specimens were sent to the Department of Pathology. Nasal packing was removed on the 2nd postoperative day; daily saline nasal spray was then performed. Topical or systemic steroids (methylprednisolone, 20 mg per day) were prescribed if mucosa edema was detected during the first follow-up visit (1 month after surgery). Postoperative follow-up endoscopy was easily scheduled 1, 6, 12, and 24 months after surgery for all patients. In addition, postoperative CT was performed 24 months after surgery to accurately assess the recurrence of ACP.
9
Nasal Mucus Viscosity Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The nasal mucus was collected by using one half of a 15 mm thin Merocel (Medtronic Xomed, Jacksonville, Fl. USA) for each nostril. The Merocels were placed in the nasal cavity for 20 minutes. Afterwards, they were centrifuged on 4500 rotations per minute at 25°C for 15 minutes. To determine the volume of nasal mucus, standardized 100 µland 10 µl-pipettes were used. Analyses of viscosity of nasal mucus were conducted by a plate rheometer (the Modular Compact Rheometer MCR 102, Anton Paar, Graz, Austria) with a CP25-1 measure plate at 37 °C. A 30 µl aliquot of the patient's nasal mucus was placed on the rheometer plate to allow equilibration for 30 seconds at 37 °C, ensuring nearly in vivo conditions. A range of oscillatory frequenciesshear rates between 0.1 and 10'000 γ -was applied to the samples at a constant strain to measure viscosity.
Viscosity was recorded at 27 different measuring points and determined at 1 rad/s as well as 100 rad/s for each
All measurements were directly conducted after mucus collection to minimize alterations in rheological characteristics.
Viscosity was recorded at 27 different measuring points and determined at 1 rad/s as well as 100 rad/s for each
All measurements were directly conducted after mucus collection to minimize alterations in rheological characteristics.
10
Multilayered Closure for Cranial Base Defects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A meticulous closure of the cranial base defect after tumor removal is of critical importance to prevent a postoperative CSF leak, pneumocephalus, and their potential complications. We perform multilayered reconstruction to achieve a watertight closure as described by most authors [Figure 7 ].[39 (link)40 (link)] As the first step, we prefer to place autologous fat in the sellar cavity to cover the arachnoid defect. Care must be taken to avoid too tight packing or too deep placement of the graft. A fascia lata graft harvested from the thigh is then placed intradurally as an underlay graft, carefully tucked underneath the dural edges. Where possible, a small piece of thin bone or cartilage obtained from the nasal septum is placed snugly over the bony defect in the skull base. The final layer of a previously prepared vascularized pedicled nasoseptal flap is then rotated to cover the entire defect. We apply a thin layer of fibrin glue (TISSEEL, Baxter Healthcare Corp.) over the flap edges to prevent its displacement or migration. The flap is supported by the nasal Merocel (Medtronic Xomed) pack which is left in place for 3–4 days. A controlled drainage of CSF using a lumbar catheter is continued postoperatively for 5 days. We have found this method of closure very effective in preventing postoperative CSF leaks.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!