Cd94 fitc

Manufactured by BioLegend

Sourced in Canada, United States

CD94-FITC is a fluorescently-labeled monoclonal antibody that binds to the CD94 cell surface receptor. CD94 is a C-type lectin-like receptor expressed on natural killer (NK) cells and a subset of T cells, and it plays a role in the regulation of immune cell function.

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3 protocols using cd94 fitc

Surface Marker Staining of γδTc

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For surface marker staining of γδTc, the following anti-human antibodies from BioLegend, unless otherwise indicated, were employed: TCRγδ PE (clone B1, 1:25); TCR Vδ1 FITC (Miltenyi, clone REA173, 1:10); TCR Vδ2 PerCP (clone B6, 1:25); NKG2D APC (BD Biosciences, Mississauga, ON, Canada, 1:25); CD56 FITC (clone MEM-188, 1:5); CD69 AF700 (clone FN50, 1:4); CD94 FITC (clone DX22, 1:5); CD95 APC (clone DX2, 1:100); HLA ABC PE (clone W6/32, 1:10); FasL PE (clone NOK-1, 1:5); and CD40L APC (clone 24–31, 1:5).
Anti-human MICA/B PE (BioLegend, clone 6D4, 0.1 µg) was used to stain breast cancer cell lines.

Siegers G.M., Dutta I., Lai R, & Postovit L.M. (2018). Functional Plasticity of Gamma Delta T Cells and Breast Tumor Targets in Hypoxia. Frontiers in Immunology, 9, 1367.

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Phenotyping Natural Killer Cells

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The following antibodies were used to stain single cell suspensions: CD3-APC (UCHT1), CD56-PE-Cy7 (MEM-188), DNAM-1-FITC (TX25), CD16-FITC (3G8), CD94-FITC (DX22), CD158a/h-FITC (HP-MA4), CD158e1-FITC (DX9), NKG2D-PE (1D11), NKp30-PE (P30-15), 2B4-PE (C1.7), CD158b-PE (DX27), NKp46-PercP-Cy5.5 (9E2) (BioLegend, San Diego, CA), and NKG2A-PE (131411) (R&D system, Minneapolis, MN). Briefly, plated cells or RBC lysed peripheral whole blood cells were incubated with antibodies for 30 min at 4°C, then washed with PBS, and fixed for 20 min using 4% paraformaldehyde. Cells were further washed twice with PBS and analyzed by flow cytometry using a FACS Canto II (BD Biosciences). Isotype antibodies were used as controls. Data was analyzed using the Flowjo software (BD Biosciences).

Han B., Mao F.Y., Zhao Y.L., Lv Y.P., Teng Y.S., Duan M., Chen W., Cheng P., Wang T.T., Liang Z.Y., Zhang J.Y., Liu Y.G., Guo G., Zou Q.M., Zhuang Y, & Peng L.S. (2018). Altered NKp30, NKp46, NKG2D, and DNAM-1 Expression on Circulating NK Cells Is Associated with Tumor Progression in Human Gastric Cancer. Journal of Immunology Research, 2018, 6248590.

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Monoclonal Antibodies Characterization

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The following mAbs were isolated in our laboratory, licensed to the indicated companies and validated for their specificity: anti-NKp30 (AZ20, IgG1), anti-NKp44 (Z231, IgG1), anti-NKp46 (BAB281, IgG1) (Beckman Coulter/Immunotech, Marseille, France). The purified anti-PD-1 mAb (PD1.3.1.3 clone, IgG2b) was originally isolated at the Laboratoire Immunologie des Tumeurs, CRCM, Marseille-Luminy (France). 31 The following purchased mAbs were used in this study: Anti-CD56-PC7 (c218 clone), and anti-NKG2A allophycocyanin (Z199 clone) were purchased from Beckman Coulter/Immunotech (Marseille, France); anti-CD3-Viogreen (BW264/56 clone), anti-CD19-VioGreen (LT20 clone), anti-CD14-Viogreen (TÜK4 clone), anti-TNF-α-PE, anti-CD223-PE (LAG-3), mAbs were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany); anti-TIM-3 was purchased from Novus Biologicals (Littleton, CO 80120, USA); anti IL-17A-FITC, CD45-APC-H7 (2D1 clone) from BD Bioscience; IL-22-PE TNF-α-eFluor450 purchased from eBioscience (San Diego, CA); CD94-FITC, CD127-BrilliantVio-let421, CD117-PerCP-Cy5.5, anti-CRTH2-APC, anti-TIGIT-PE purchased from BioLegend (San Diego, CA); IL-8-PE purchased from R&D (Minneapolis, MN) and anti-B7-H3 (D-8 clone, IgG2b) was purchased from Santa Cruz Biotechnology (Dallas, TX).

Vacca P., Pesce S., Greppi M., Fulcheri E., Munari E., Olive D., Mingari M.C., Moretta A., Moretta L, & Marcenaro E. (2019). PD-1 is expressed by and regulates human group 3 innate lymphoid cells in human decidua. Mucosal immunology, 12(3).

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