Bovine serum albumin (BSA), trypsin from porcine pancreas, α-chymotrypsin from bovine pancreas, and histofine were purchased from Nacalai Tesque (Kyoto, Japan). Carboxyfluorescein diacetate succinimidyl ester (CFSE) was from Invitrogen (Carlsbad, CA). Pentobarbital sodium was from Kyoritsu Seiyaku (Tokyo, Japan). Biotin-conjugated anti-Pseudomonas species polyclonal antibody, anti-MHC-II monoclonal antibody (OX-6) and anti-keratin 10 mouse monoclonal antibody (DE-K10) were from Thermo Fisher Scientific (Waltham, MA). Anti-Ki 67 mouse monoclonal antibody (MIB-5) was from Dako (Glostrup, Denmark). VectaStain ABC Kit and biotin-conjugated anti-mouse IgG antibody were from Vector Laboratories (Burlingame, CA), and 3,3′-diaminobenzidine tetrahydrochloride (DAB Tablet) was purchased from Wako Pure Chemical (Osaka, Japan).
3 3 diaminobenzidine tetrahydrochloride dab tablet
Manufactured by Fujifilm
Sourced in Japan
3,3′-diaminobenzidine tetrahydrochloride (DAB Tablet) is a chromogenic substrate used in immunohistochemistry and other laboratory techniques to detect the presence of specific target molecules or antigens. It is a stable, solid tablet form of the DAB compound, which produces a brown-colored precipitate upon enzymatic reaction, allowing for the visualization and localization of the target of interest.
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2 protocols using 3 3 diaminobenzidine tetrahydrochloride dab tablet
1
Immunohistochemical Analysis of Pseudomonas Infection
2
Embryonic Cadherin 11 Expression in Mice
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E16.5, E18.5 embryos were obtained from the uterus of pregnant C57BL/6J mice anaesthetized via intraperitoneal injection with 2,2,2-tribromoethanol (250 mg/kg body weight). Dissected cranial bases from E16.5, E18.5 and postnatal day 2 (P2) embryos anesthetized on ice were fixed in 4% paraformaldehyde in PBS overnight at 4 °C. Tissues were transferred to 15 and 30% sucrose in PBS for several hours. Tissues in 30% sucrose and OCT compound mixture (1:1) were frozen and stored at -80 °C. Sagittal sections with a thickness of 20 μm were used. Rabbit polyclonal anti-cadherin 11 antibody (1:100; Thermo Fisher, 71-7600) was used for primary antibody. The biotin-streptavidin (B-SA) staining method with the Histofine immunostaining kit (Nichirei, Tokyo Japan) and 3,3'-diaminobenzidine, tetrahydrochloride (DAB) tablet (WAKO, Osaka Japan) was utilized for detection.
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