Axiovert 200 m wide field fluorescence microscope

Manufactured by Hamamatsu Photonics

Sourced in United States

The Axiovert 200 M wide-field fluorescence microscope is a laboratory equipment designed for high-performance fluorescence imaging. It features a stable optical system and a versatile design to support a wide range of applications. The core function of this microscope is to enable detailed observation and analysis of samples through the use of fluorescence-based techniques.

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Lab products found in correlation

C4742 95 ccd camera, by Hamamatsu Photonics (2 mentions) Volocity software, by PerkinElmer (2 mentions) Orca flash4.0 lt camera, by Hamamatsu Photonics (1 mentions) Zen v 2, by Zeiss (1 mentions)

Close spelling variants of this product

Axiovert 200 microscope Axiovert 200 Axiovert microscope Fluorescence microscope

3 protocols using axiovert 200 m wide field fluorescence microscope

Intracellular and Extracellular Localization of Parasite Proteins

Cited 2 times

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For intracellular localization, parasites were inoculated into 6-well plate having coverslips confluent with HFF cells. Following overnight incubation, parasites were fixed with 100% methanol. For extracellular localization, freshly lysed parasites were filtered, pelleted, and resuspended in PBS. Thereafter, parasites were added to poly-L-lysine coated cover-slips and allowed to incubate for 30 min at 4°C prior to fixation with 100% methanol. 1% BSA fraction V in PBS was used as blocking agent.
The following primary antisera were used: α-Myc MAb 9E10 (1:50) (Santa-Cruz Biotech), mouse α-Ty (1:500; kindly provided by Chris de Graffenried, Brown University), rabbit α-IMC3(1-120) [1:2,000 (Anderson-White et al., 2011 (link))], mouse α-GFP (Abgent; 1:500). Guinea pig α-AAP4 [1:200 (Engelberg et al., 2020 (link))]. Alexa 488 (A488) or A594 conjugated goat α-mouse, α-rabbit, or α-guinea pig were used as secondary antibodies (1:500) (Invitrogen). DNA was stained with 4’,6-diamidino-2-phenylindole (DAPI). A Zeiss Axiovert 200 M wide-field fluorescence microscope equipped with a α-Plan-Fluar 100x/1.3 NA and 100x/1.45 NA oil objectives and a Hamamatsu C4742-95 CCD camera was used to collect images, which were deconvolved and adjusted for phase contrast using Volocity software (Perkin Elmer, USA).

Gubbels M.J., Ferguson D.J., Saha S., Romano J.D., Chavan S., Primo V.A., Michaud C., Coppens I, & Engelberg K. (2022). Toxoplasma gondii’s Basal Complex: The Other Apicomplexan Business End Is Multifunctional. Frontiers in Cellular and Infection Microbiology, 12, 882166.

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Fluorescence Microscopy of Tox oplasma Parasites

Cited 3 times

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Parasites were seeded overnight (∼16–18 h) and fixed with methanol as previously described (Gubbels et al., 2006 (link)). The following antibodies were used in this study: Myc (MAb 9E10, mouse, 1:50; Santa Cruz); HA (3F10, rat; 1:3000; Roche); TgIMC3 (rat, 1:2000; Anderson-White et al., 2011 (link)); TgNuf2 and TgNdc80 (guinea pig, 1:2000; Farrell and Gubbels, 2014 (link)); SFA (rabbit, 1:1000; kindly provided by Boris Striepen, University of Georgia; Francia et al., 2012 (link)); TgNek1 (1:1000; Chen and Gubbels, 2013 (link)); HsCentrin (rabbit, 1:1000; kindly provided by Iain Cheeseman, Whitehead Institute); Ty (mouse, 1:1000; kindly provided by Sebastian Lourido); TgEB1 (rat, 1:3000; this work). Alexa Fluor A488, A568, A594, A633, and A647 secondary antibodies were used. We used 4′,6′-diamidino-2-phenylindole (DAPI) to stain nuclear material. Images were acquired using a Zeiss Axiovert 200M wide-field fluorescence microscope equipped with a Plan-Fluor 100×/1.45 NA oil objective and a Hamamatsu Orca-Flash 4.0LT camera. For superresolution structured illumination microscopy (SIM), a Zeiss Elyra S.1 microscope equipped with a Plan-Apochromat 63×/1.40 oil objective and a PCO-Tech pco.edge 4.2 sCMOS camera in the Boston College Imaging Core was used in consultation with Bret Judson. Images were acquired and processed in Zeiss ZEN v. 2.3 software using the standard mode.

Chen C.T, & Gubbels M.J. (2019). TgCep250 is dynamically processed through the division cycle and is essential for structural integrity of the Toxoplasma centrosome. Molecular Biology of the Cell, 30(10), 1160-1169.

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Immunofluorescence Imaging of Intracellular Parasites

Cited 1 time

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Immunofluorescence assays were performed as previously described^{51 (link)}. Parasite strains of choice were inoculated in six-well plates containing coverslips with HFF cells and fixed with 100% methanol and blocked in 1% BSA in PBS. Alternatively, extracellular parasites were adhered on coverslips coated with poly-Lysine for 1 hr at 37°C. The following primary antibodies were used: rat α-IMC3 1:2000^{49 (link)}, mouse MAb 45:36 α-IMC1 1:1000 (kindly provided by Gary Ward, University of Vermont), α-Myc conjugated with A-488 (Cell Signaling) and rabbit α-human centrin 1:1000 (kindly provided by Iain Cheeseman, Whitehead Institute). Alexa fluorophores A488 and A594 (Invitrogen) conjugated to α-rat, α-rabbit, and α-mouse secondary antibodies were used. Nuclear material was co-stained with 4′6-diamidino-2-phenylindole (DAPI). A Zeiss Axiovert 200 M wide-field fluorescence microscope equipped with a α-Plan-Fluar 100×/1.45 NA oil objective and a Hamamatsu C4742-95 CCD camera was used to collect images, which were deconvolved and adjusted for phase-contrast using Volocity software (Improvision/Perkin Elmer).

Dubey R., Staker B.L., Foe I.T., Bogyo M., Myler P.J., Ngô H.M, & Gubbels M.J. (2016). Membrane skeletal association and post-translational allosteric regulation of Toxoplasma gondii GAPDH1. Molecular microbiology, 103(4), 618-634.

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