Plastic cell culture dishes and plates

Baohuoside I was obtained from the Nanjing Zelang Medical Technology Co. Ltd (Nanjing, PR China) and purity was > 98%. TPGS, DDAB, and HA were purchased from Aladdin Industrial Co. Ltd (Liguria, Italy). Plastic cell culture dishes and plates were purchased from Corning Incorporated (Corning, NY). Coumarin-6 and DAPI were purchased from Sigma-Aldrich (Shanghai, PR China). DiR was purchased from Ganhua Trade Co. Ltd. (Shanghai, PR China). All reagents were of analytical grade except methanol, which was of chromatographic grade.

Mesoporous silica with a mean particle diameter of 150 nm was purchased from Nanjing XFNANO Materials Tech Co., Ltd., (Nanjing, China). The dopamine hydrochloride was purchased from Alfa Aesar (Ward Hill, MA, USA) while the NGR was synthesized by the Chinese Peptide Company (China). The DOX and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise specified. The plastic cell culture dishes and plates were purchased from Corning Inc., (Corning, NY, USA). The anti-CD31 and anti-CD13 antibodies were purchased from Abcam (MA, USA) while the anti-CD13 (fluorescein isothiocyanate, FITC) was purchased from Biorbyt (Cambridgeshire, UK). The primary BCEC cultures and astrocytes cells (AC) were prepared from 4-week- and 3-day-old Sprague-Dawley male rats, respectively, provided by the Experimental Animal Center of Chongqing Medical University. The C6, GL261, U251 cells were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). All animal experiments were performed according to the protocol evaluated and approved by the Ethics Committee of Chongqing Medical University.