The Boston Children’s Hospital IACUC approved all experiments involving mice in this study. Adult CD-1 male and female mice and pregnant CD-1 dams were obtained from Charles River Laboratories. Sprague Dawley rats were purchased from Charles River Laboratories. Animals were housed in a temperature- and humidity-controlled room (70 ± 3°F, 35–70% humidity) on a 12 h light/12 h dark cycle (7 a.m. on, 7 p.m. off) and had free access to food and water. All animals younger than postnatal day 10 were allocated into groups based solely on gestational age without respect to sex (both males and females were included). For studies involving rodents older than 10 days, sex was treated as a variable.
Adult cd 1 male and female mice
Manufactured by Charles River Laboratories
Sourced in United States
Adult CD-1 male and female mice are laboratory animals commonly used in various research applications. They are bred and maintained for experimental purposes.
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Lab products found in correlation
2 protocols using adult cd 1 male and female mice
1
Rodent Housing and Handling Protocol
2
Mice Handling and Euthanasia Protocol
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Adult CD1 male and female mice (20–25 g) were purchased from Charles River Laboratories (Kingston, NY, USA) and allowed to acclimate to the environment before using them for experiments. Animals were kept in a facility with controlled temperature and humidity regulation; food and water were provided ad libitum. All procedures were performed based on the approval of the respective protocols from Institutional Animal Care and Use Committee at the University of Nebraska-Kearney (#060916) and University of Cincinnati (#041416). Institutional guidelines for the proper and humane use of animals for research were followed. As formalin is toxic to tissues, mice were euthanized immediately after the procedure by using carbon dioxide inhalation, followed by cervical dislocation. Experiments were performed when the animals were between 6 and 12 weeks old.
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