Dxm 1096 microscan walkaway

Ascitic fluid samples were stained using Gram stain and cultured on HK semi-fluid plates (Kyokuto Pharmaceutical Co., Tokyo, Japan), Brucella HK (RS) agar plates (Kyokuto Pharmaceutical Co.), chocolate agar plates No. 2 (Kyokuto Pharmaceutical Co.), and Trisoy blood agar plates (sheep) No. 2 (Kyokuto Pharmaceutical Co.). Depending on the results of Gram staining, a DHL (Deoxycholate Hydrogen sulfide Lactose) agar plate (Kyokuto Pharmaceutical Co., Tokyo, Japan) and sheep blood plate (Nissui Pharmaceutical Co., Ltd., Tokyo) were added to the culture. Enterococci cultured on blood agar plates were identified by the MicroScan WalkAway 40 plus System (Beckman Coulter Japan, Tokyo, Japan) until November 6, 2016, and, thereafter, matrix-assisted laser desorption ionization-time-of-flight mass spectrometry on a MALDI Biotyper (Bruker Daltonics Co. Ltd., Billerica, MA, USA). Susceptibility testing was performed using the MicroScan WalkAway 40 plus System until October 14, 2018; thereafter, the DxM 1096 Microscan WalkAway (Beckman Coulter Inc., Carlsbad, CA, USA). The interpretive criteria used to determine antibiotic susceptibility were in accordance with the CLSI’s susceptibility testing standards for enterococci, document M100-S22 (2012) [15 ]. M100-S22(2012) has not changed breakpoints with ampicillin and vancomycin by the current M100-Ed33(2023) [16 ].

JICS135 was isolated from one of two sets of blood culture taken from an inpatient in 2014. The inpatient was a 77 year-old man with chronic kidney disease who required a long-term internal catheter. Blood cultures were processed using the BacTAlert system (bioMe´rieux, Basingstoke, UK) at Saiseikai Yokohamashi Tobu Hospital in Japan. Identification and minimal inhibitory concentrations (MICs) of antibiotics were determined by DxM 1096 MicroScan WalkAway (Beckman Coulter, U.S.) based on Clinical and Laboratory Standards Institute (CLSI) guidelines (M100S, 26^th edition).