Western blotting electrophoresis apparatus

PCR amplification and transilluminator (both from Bio-Rad, Berkeley, CA, USA), electrophoresis apparatus (Beijing Liuyi Biotechnology Co., Ltd., Beijing, China), centrifuge and micro-pipette (both from Eppendorf AG, Hamburg), Haier ice maker, western blotting electrophoresis apparatus (Bio-Rad), −80°C freezer (Thermo Fisher Scientific, Waltham, MA, USA), 10 ml syringe, 5 ml syringe (Tianjin Hanaco Medical Co., Ltd., Tianjin, China), surgical instruments for use on laboratory animals (Beijing Medical Device Factory, Beijing, China), NanoDrop2000 photometric analyzer (Thermo Fisher Scientific, Wilmington, DE, USA), Eppendorf tube (Eppendorf AG), water bath (Beijing Medical Device Factory), and pathology microtome (Leica, Mannheim, Germany).

Equipment used for the polymerase chain reaction (PCR) included amplification instrument (Bio-Rad, Berkeley, CA, USA), gel imager (Bio-Rad), electrophoresis apparatus (Beijing Liuyi Instrument Factory, Beijing, China), centrifuge (Eppendorf AG, Hamburg, Germany), trace pipettes (Eppendorf AG), and a Haier ice making machine (Haier Company, Shanghai, China). Equipment used for western blotting included western blotting electrophoresis apparatus (Bio-Rad), −80°C refrigerator (Thermo Fisher Scientific, Waltham, MA, USA), 10 ml syringe, 5 ml syringe (Tianjin Hanaco Medical Co., Ltd., Tianjin, China) and for the animal experiments special surgical instruments (Beijing Medical Instrument Factory, Beijing, China), NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific), Eppendorf tube (Eppendorf AG), and water bath (Beijing Medical Instruments Factory).