The following primary antibodies (anti-human) were used: AKT, phospho-AKT, MAPK(ERK1/2), phospho-MAPK(ERK1/2), Jak2, phospho-Jak2, Stat3, and phospho-Stat3 (Cell Signaling Technology, MA, USA), GAPDH (Multisciences, China), 4H7 (Soochow University-Bright Scistar, China), CD276 (R&D Systems, MN, USA), and CXCR4 (EMD Millipore, MA, USA).
The following secondary antibodies were used: horseradish peroxidase-conjugated rabbit anti-goat, goat anti-mouse, and anti-rabbit antibodies (Multisciences, China). Proteins were visualized with an ECL detection kit (Bio-Rad, CA, USA). The following fluorescent secondary antibodies were used: Cy3-conjugated anti-goat IgG (Invitrogen, CA, USA) and Alexa Fluor 633-conjugated anti-rabbit IgG (Invitrogen, CA, USA). The following antibodies were used for flow cytometry: B7-H3-APC (eBiosciences, CA, USA) and CXCR-4-PE-cy7 (eBiosciences, CA, USA).
The following secondary antibodies were used: horseradish peroxidase-conjugated rabbit anti-goat, goat anti-mouse, and anti-rabbit antibodies (Multisciences, China). Proteins were visualized with an ECL detection kit (Bio-Rad, CA, USA). The following fluorescent secondary antibodies were used: Cy3-conjugated anti-goat IgG (Invitrogen, CA, USA) and Alexa Fluor 633-conjugated anti-rabbit IgG (Invitrogen, CA, USA). The following antibodies were used for flow cytometry: B7-H3-APC (eBiosciences, CA, USA) and CXCR-4-PE-cy7 (eBiosciences, CA, USA).