Female C57BL/6J wild-type mice were purchased from Charles River (Sulzfeld, Germany) or Janvier Laboratories (Le Genest-Saint-Isle, France) at the age of 6-8 weeks and were kept under pathogen-controlled conditions in the center for preclinical studies at German Cancer Research Center (DKFZ, Heidelberg, Germany). Animal experiments were performed according to the rules of the German Animal Welfare Act and were licensed by the local authorities (Regierungspräsidium Karlsruhe).
Wild type c57bl 6j female mice
Manufactured by Charles River Laboratories
Sourced in Germany
Wild-type C57BL/6J female mice are a commonly used inbred mouse strain. They are a standard model organism for biomedical research.
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7 protocols using wild type c57bl 6j female mice
1
C57BL/6J Mice in Preclinical Studies
2
Wound Healing in C57Bl/6J Mice
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Female C57Bl/6 J (wild-type) mice were obtained from Charles River Laboratories (Charles River Laboratories, Sulzfeld, Germany). At the age of 12 weeks, the mice were caged individually, monitored for body weight, and wounded as described in the following subsection.
3
Intranasal Inoculation of C57Bl/6J Mice
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Female C57Bl/6J wild-type (WT) mice were purchased from Charles River Inc. (Maastricht, The Netherlands). Mice were housed under specific pathogen-free conditions receiving food and water ad libitum. Age-matched mice were used in all experiments. The Animal Care and Use Committee of the University of Amsterdam approved all experiments (permit numbers: DIX102020AQ and DIX102791). Intranasal inoculation was performed under isoflurane anesthesia and mice were euthanized under general anesthesia, all efforts were made to minimize suffering.
4
Wildtype and Transgenic Mouse Models
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Female C57BL/6 J wildtype (WT) mice (7–14 weeks at the beginning of the experiments) were purchased from Charles River (Sulzfeld, Germany). OT-I mice (8–14 weeks at the beginning of the experiments) producing CD8+ T cells that express a transgenic T cell receptor specific for the chicken ovalbumin (OVA) epitope SIINFEKL (OVA257-264) presented on MHC class I H–2 Kb and the congenic marker CD90.1 were bred and held in house. All mice were kept under specific pathogen-free conditions at the Central Animal Laboratory (Nijmegen, The Netherlands). Drinking water and standard laboratory food pellets were provided ad libitum.
5
Transgenic Mouse Bone Marrow Transfer
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Transgenic C57BL/6J mice expressing enhanced GFP (UBC‐GFP) were obtained from Jackson Laboratory (Bar Harbor, ME, USA) Jand used as bone marrow or uterine cell donors. Wild‐type C57BL/6J female mice were obtained from Charles River Laboratories (Wilmington, MA, USA) and used as recipients of bone marrow or uterine cells injection. All animals were maintained in the Animal Facility of Yale University School of Medicine. Mice were housed 4–5 per cage in an animal room exposed to a 12‐hrs light/dark cycle (7:00 a.m.–7:00 p.m.) with food and water provided ad libitum. All animals were treated under an approved Yale University institutional animal care and use committee protocol.
6
Mouse model for Cystinosis study
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Wild type C57BL/6J male mice, along with wild‐type C57BL/6J female mice for the mating experiments, were purchased from Charles River Laboratories, France. Knockout (KO) Ctns−/− male mice with C57BL/6J background (C57BL/6J‐Ctns−/−) were obtained as a compliment from Prof. C. Antignac, Laboratory of Hereditary Kidney Disease, Imagine Institute, INSERM U1163, Université de Paris, Paris, France.12
7
In Vivo Nanoparticle Tracking with Multimodal Imaging
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Wild-type C57Bl/6J female mice were obtained from Charles River Laboratory and maintained under specific pathogen-free conditions at the Central Animal Laboratory in Nijmegen, Netherlands. Experiments were performed according to the guidelines for animal care of the Nijmegen Animal Experiments Committee (DEC 2013-243).
Prior to the experiment, mice were shaved to avoid hair absorption and to facilitate the injections and imaging sessions. 40 µl of nanoparticles in PBS at concentration 100 mg/mL or 0.1 x106 and 1x106 cells (in 30 µl PBS) loaded with nanoparticles were injected into a mouse thigh muscle under ultrasound guidance, followed by the photoacoustic and fluorescent imaging. Furthermore, free nanoparticles and nanoparticle-loaded cells (0.1 x106 and 1x106) were injected intramuscularly, followed by 19F MR imaging.
Prior to the experiment, mice were shaved to avoid hair absorption and to facilitate the injections and imaging sessions. 40 µl of nanoparticles in PBS at concentration 100 mg/mL or 0.1 x106 and 1x106 cells (in 30 µl PBS) loaded with nanoparticles were injected into a mouse thigh muscle under ultrasound guidance, followed by the photoacoustic and fluorescent imaging. Furthermore, free nanoparticles and nanoparticle-loaded cells (0.1 x106 and 1x106) were injected intramuscularly, followed by 19F MR imaging.
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