A5708

Manufactured by ABclonal

Sourced in China

A5708 is a laboratory equipment that serves as a Benchtop Centrifuge. It is designed for general purpose applications that require centrifugation of samples.

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Lab products found in correlation

3 protocols using a5708

Evaluating Chondrocyte Protein Expression

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We detected the expression levels of RAD54L, HIF-1α, and VEGF with the help of Western Blot assay. Proteins extracted from chondrocytes or cartilage tissues were isolated using a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Millipore, Danvers, MA, USA). The membrane blocked by 5% skim milk was exposed to primary antibodies which included anti-RAD54L (A20181, 1:1000, ABclonal, Wuhan, China), anti-HIF-1α (ab179483, 1:1000, Abcam), anti-VEGF (A5708, 1:1000, ABclonal), and anti-GAPDH (ab181602, 1:10000, Abcam) antibodies at 4°C overnight. The membrane was then incubated with a horseradish peroxidase-conjugated anti-rabbit secondary antibody (ab288151, 1:5000, Abcam) at room temperature for 1 h. The proteins after exposure were visualized using the Tanon 5200 chemiluminescence imaging system (Tanon, Shanghai, China).

Li Z., Xie L., Zou L., Xiao S, & Tao J. (2024). Overexpression of RAD54L attenuates osteoarthritis by suppressing the HIF-1α/VEGF signaling pathway: Bioinformatics analysis and experimental validation. PLOS ONE, 19(4), e0298575.

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Angiogenesis Signaling Pathway Regulation

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The reagents used for study included RIPA buffer (high) (R0010, Solarbio, Beijing, China), BCA protein assay kit (P0010, Beyotime Biotechnology, Shanghai, China), phosphatase inhibitor (M7528, AbMole, Shanghai, China), anti-CD34 antibody (GB121693, Servicebio, Wuhan, China), anti-PI3K antibody (4249, Cell Signaling Technology (CST), USA), anti-AKT antibody (4691, CST, USA), anti-pAKT antibody (4060, CST, USA), anti-VEGF antibody (A5708, ABclonal, Wuhan, China), GAPDH antibody (AC033, ABclonal, Wuhan, China), goat anti-rabbit IgG (AS014, ABclonal, Wuhan, China) and goat anti-mouse IgG (AS003, ABclonal, Wuhan, China).
The instruments employed used included a light microscope (AxioScope.A1, Zeiss, Germany), photomicroscope (AxioVert.A1, Zeiss, Germany), slice scanner (KF-PRO-005-EX, Ningbo, China), high-speed low-temperature tissue grinding instrument (KZ-III-F, Servicebio, Wuhan, China) and electrophoresis system (Mini-Protean system, BioRad, USA).

Zhang L., Li H., Zhang L., Zu Z., Xu D, & Zhang J. (2023). Network Pharmacology Analysis of the Mechanisms Underlying the Therapeutic Effects of Yangjing Zhongyu Tang on Thin Endometrium. Drug Design, Development and Therapy, 17, 1805-1818.

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Antibodies for Western Blot and IHC Analysis

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Anti‐HIF‐1α (WB 1:500; IHC 1:100; ab51608), anti‐METTL3 (WB 1:1,000; IHC 1:500; ab195352), anti‐FOXO3 (WB 1:1,000; ab53287), goat anti‐rabbit IgG (WB 1:2,000; ab6721), and goat anti‐rabbit IgG‐FITC (IF 1:200, ab6717) antibodies were purchased from Abcam, USA. Anti‐MAP1LC3‐B (WB 1:1,000; IHC 1:100; IF 1:100; A7198), anti‐PDGF‐B (WB 1:1,000; A1195), anti‐VEGF‐A (WB 1:1,000; IHC 1:100; A5708), anti‐Ki67 (IHC 1:100; A11390), anti‐GAPDH (WB 1:1,000; AC027), anti‐tubulin (WB 1:5,000; AC021), anti‐β‐actin (WB 1:50,000; AC026), anti‐YTHDF1 (IHC 1:1,000; A18126), and anti‐YTHDF2 (IHC 1:1,000; A15616) antibodies were purchased from Abclonal, China. Anti‐m⁶A (MeRIP 1:1,000; ABE572) antibody was purchased from Merck Millipore (Massachusetts, USA). Western blot analyses were performed by standard methods described previously (Niu et al, 2019).

Lin Z., Niu Y., Wan A., Chen D., Liang H., Chen X., Sun L., Zhan S., Chen L., Cheng C., Zhang X., Bu X., He W, & Wan G. (2020). RNA m6A methylation regulates sorafenib resistance in liver cancer through FOXO3‐mediated autophagy. The EMBO Journal, 39(12), e103181.

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