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N storm inverted tirf microscope

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The N-STORM inverted TIRF microscope is a high-resolution imaging system designed for advanced fluorescence microscopy applications. It utilizes total internal reflection fluorescence (TIRF) illumination to selectively excite fluorophores near the coverslip-sample interface, enabling high-contrast imaging of cellular structures and dynamics. The N-STORM microscope is capable of achieving single-molecule localization and super-resolution imaging, providing researchers with a powerful tool for exploring the nanoscale features of biological samples.

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Inverted microscope (910 citations) N-STORM (43 citations) N-STORM microscope (31 citations) TIRF microscope (24 citations) N-STORM inverted microscope (2 citations)

2 protocols using n storm inverted tirf microscope

1

STORM Imaging of Lipid Assemblies

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Mixtures of PC-C12 (19 μM) and 5CB were used for STORM. ATTO 488-labeled PE-C14 (10 % mol/mol based on PC-C12) was also doped into the mixture as a fluorescent probe for STORM imaging50 . Optical cells were prepared by using polyimide-coated #1.5 coverslips. Imaging was carried out on a Nikon N-STORM inverted TIRF microscope with 405 nm and 488 nm laser excitation. Images were collected with an Andor iXon Ultra 897 EMCCD camera and controlled through NIS-Elements Ar software with N-STORM analysis. Images were acquired after an initial 1 min pre-exposure (to allow the transition of the majority of fluorophores into a meta-stable dark state) followed by a 5–10 min acquisition period at 17 ms/frame. The apparent widths of the assemblies formed in LC defects were determined by measuring the full width at half maximum (FWHM) of the assemblies.
Wang X., Miller D.S., Bukusoglu E., de Pablo J.J, & Abbott N.L. (2015). Topological Defects in Liquid Crystals as Templates for Molecular Self-Assembly. Nature materials, 15(1), 106-112.
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2

STORM Imaging of Lipid Assemblies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mixtures of PC-C12 (19 μM) and 5CB were used for STORM. ATTO 488-labeled PE-C14 (10 % mol/mol based on PC-C12) was also doped into the mixture as a fluorescent probe for STORM imaging50 . Optical cells were prepared by using polyimide-coated #1.5 coverslips. Imaging was carried out on a Nikon N-STORM inverted TIRF microscope with 405 nm and 488 nm laser excitation. Images were collected with an Andor iXon Ultra 897 EMCCD camera and controlled through NIS-Elements Ar software with N-STORM analysis. Images were acquired after an initial 1 min pre-exposure (to allow the transition of the majority of fluorophores into a meta-stable dark state) followed by a 5–10 min acquisition period at 17 ms/frame. The apparent widths of the assemblies formed in LC defects were determined by measuring the full width at half maximum (FWHM) of the assemblies.
Wang X., Miller D.S., Bukusoglu E., de Pablo J.J, & Abbott N.L. (2015). Topological Defects in Liquid Crystals as Templates for Molecular Self-Assembly. Nature materials, 15(1), 106-112.
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