Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody

Anti-MMP-12, anti-MMP-9, anti-GFAP, anti-MOG, anti-Iba1, anti-TNFα, anti-TNFR1, anti-TNFR2, anti-caspase-3, anti-cytochrome C, anti-AIF, and anti-MBP antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-NeuN antibody was obtained from Millipore (Billerica, MA). Anti-glial fibrillary acidic protein (GFAP) was obtained from Dakocytomation (Carpinteria, CA). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was obtained from Novus Biologicals (Littleton, CO).

Throughout the experiments, the following primary antibodies were used: RasGRP3 and phospho-RasGRP3 (Thr133); p44/42 MAP kinase (ERK 1/2) and phospho-p44/42 MAP kinase (phospho-ERK 1/2); Akt and phospho-Akt (Ser473) antibodies for Western blot experiments were obtained from Cell Signaling Technology (Beverly, MA). Anti-RasGRP3 antibody for immunohystochemistry was purchased from Abcam (Cambridge, UK). Ki67 antibody was obtained from DAKO (Glostrup, Denmark). Actin β, ERα, p-ERα (Ser 118) and HER-2 antibodies were purchased from Sigma-Aldrich (St. Louis, MO). IGF-IR and EGFR antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was obtained from Novus Biologicals (Cambridge, UK). Appropriate secondary antibodies were purchased from Bio-Rad Laboratories (Hercules, CA).