Asserachrom protein c

Manufactured by Diagnostica Stago

Sourced in France

The Asserachrom Protein C is a laboratory instrument designed to measure the levels of Protein C in a patient's blood sample. Protein C is a naturally occurring anticoagulant that plays a crucial role in the body's blood clotting process. The Asserachrom Protein C provides quantitative analysis of Protein C levels, which can aid healthcare professionals in the diagnosis and management of various coagulation disorders.

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Lab products found in correlation

Berichrom protein c, by Siemens (2 mentions) Bcs xp, by Siemens (2 mentions) Liatest, by Diagnostica Stago (1 mentions) Sta staclot, by Diagnostica Stago (1 mentions) Innovance free ps ag, by Siemens (1 mentions)

4 protocols using asserachrom protein c

Plasma Protein C Deficiency Phenotyping

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The tests were performed at least 3 months after the thromboembolic event and after temporary withdrawal of vitamin K antagonist (for at least 10 days) or direct oral anticoagulant (NOAC; for at least 24 h). The probands were screened for thrombophilia as described [9 (link)].
Plasma PC activity was measured in citrated plasma using a chromogenic assay (Berichrom Protein C, Siemens Healthcare Diagnostics, Erlangen, Germany; reference range 70–140%) and a clot-based assay (Protein C coag, Siemens Healthcare Diagnostics; reference range 70–140%). PC antigen was assessed in citrated plasma by an enzyme-linked immunosorbent assay (Asserachrom Protein C, Diagnostica Stago, Asnieres, France; reference range 70–140%). PC deficiency was classified as previously described [9 (link)], i.e., type I-quantitative deficiency with a decreased synthesis of abnormal protein, which results in decreased activity and plasma concentration of PC, and type II-qualitative deficiency (decline in PC activity).

Weronska A., Potaczek D.P., Oto J., Medina P., Undas A, & Wypasek E. (2022). A Series of 14 Polish Patients with Thrombotic Events and PC Deficiency-Novel c.401-1G>A PROC Gene Splice Site Mutation in a Patient with Aneurysms. Genes, 13(5), 733.

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Quantitative Determination of Hemostatic Factors

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Quantitative determination of protein C antigen (%) was performed using a commercially available enzyme immunoassay Asserachrom Protein C (Diagnostica Stago, Asnieres, France). Protein C activity (%) was measured with a Clotting Assay STA-Staclot (Diagnostica Stago). Total protein S (%) was measured with a microlatex particle-mediated immunoassay Liatest (Diagnostica Stago). An enzyme-linked immunosorbent assay Imulise Monoclonal (Diagnostica Stago) was used to determine free protein S (%). A clotting assay (Bioclot; Diagnostica Stago) was used to study the activity of protein S (%). Quantitative determination of sEPCR (ng/mL) was performed by an enzyme immunoassay (Asserachrom; Diagnostica Stago, Asnieres, France). Quantitation of sTM (ng/mL) was determined by an enzymelinked immunosorbent assay (Imubind; American Diagnostica Inc, Stamford, Connecticut). The coefficients of variation (both interassay and intra-assay reproducibility) were within 6.1% except for protein S activity where they were less than 11.8%.
All laboratory procedures were carried out in full compliance with the tests used. The indicators were determined twice in each sample, taking the average value. Refreezing of samples was not allowed.

Negreva M., Georgiev S, & Vitlianova K. (2017). Decreased Activity of the Protein C Anticoagulant Pathway in the Early Hours of Paroxysmal Atrial Fibrillation. Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis, 23(7).

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Quantitative Protein C Activity Assay

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The quantitative determination of functionally active PC in plasma via chromogenic assay (Berichrom ® Protein C, Siemens Healthcare Diagnostic, Marburg, Germany) on an automatic analyser BCS XP (Siemens Healthcare Diagnostics, Marburg, Germany) in citrated plasma was performed.
In that test the examined blood sample was activated by a specific snake venom activator and then the reaction with a chromogenic substrate (pyroglutamic acid-proline-arginine-methoxy-nitroanilide) was conducted. The activity was assayed in a kinetic test by measuring the increase of the absorbance value at a wavelength of 405 nm. The reference range for PC activity in accordance to the manufacturer's recommendation was 70-140%. PC antigen was determined in citrate plasma using an antigenic assay for the quantitative determination of PC by the enzyme-linked immunosorbent assay (ELISA, Asserachrom ® Protein C, Diagnostica Stago, Asnieres-Sur-Seine, France). In that test the PC was captured by specific rabbit anti-human PC antibodies which were coupled with peroxidase bound to the remaining free antigenic determinants of PC. All unbound material was washed away and a peroxidase enzyme substrate (tetramethylbenzidine) was added. The intensity of the color was directly proportional to the level of PC. The reference range for PC according to the manufacturer's recommendation was 70-140%.

Kościelniak B., Wypasek E, & Undas A. (2015). Determinants of Elevated Levels of Natural Anticoagulants in Healthy Subjects. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 24(5).

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Quantification of Free Protein S Antigen

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The level of free PS antigen was assessed in citrated plasma on automated coagulation analyzer BCS XP (Siemens Healthcare Diagnostics, Germany) using an immunoturbidimetric assay (INNOVANCE ® Free PS Ag, Siemens Healthcare Diagnostic, Marburg, Germany). To measure free PS antigen the polystyrene particles with two specific to free PS antigen monoclonal antibodies were used. When the examined sample contained free PS antigen aggregates were created and the degree of aggregation was measured turbidimetrically. The levels of free PS were directly proportional to the turbidity. The reference ranges for the levels of free PS antigen were 67-139% for male and 60-114% for female. In order to verify the elevated levels of free PS, the measurement were repeated in citrate plasma using an antigenic assay for the quantitate determination of free PS by the ELISA (Asserachrom Protein C, Diagnostica Stago, Asnieres-Sur-Seine, France). In that test the free PS was simultaneously captured by the monoclonal antibody immobilized in the microwells and by the monoclonal antibody coupled with peroxidase. The bound enzyme peroxidase was detected via its reaction with the substrate (tetramethylbenzidine). The intensity of the color was directly proportional to the level of free PS. The reference ranges were 60-130% for male and 58-111% for female.

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