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Sialomucin

Manufactured by Yuanye Bio-Technology
Sourced in China

Sialomucin is a type of glycoprotein found in the mucus secretions of various organs. It functions as a lubricant and protective agent, helping to maintain the integrity of epithelial surfaces.

Automatically generated - may contain errors

2 protocols using sialomucin

1

Protein Expression and Purification Protocol

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N protein was manufactured by Sino Biological Inc. (Beijing, China). Epichlorohydrin, sodium hydroxide, isopropanol, tris hydroxymethyl aminomethane, and toluene were provided by China National Pharmaceutical Group Co., Ltd. (Beijing, China). DNAs were bought from TaKaRa Bio. Inc. (Dalian, China). Serum albumin was brought from Shandong Sikejie Biotechnology Co., Ltd. (Binzhou, China) β-cyclodextrin was supplied by Sigma–Aldrich Co., Ltd. (Shanghai, China). T4 polynucleotide kinase, lysozyme, Exo I, and 10 × Exo I buffer (67 mM MgCl2, 10 mM dithiothreitol, pH = 9.5, 670 mM glycine-KOH) were provided by Sangon Biotech Co. Ltd. (Shanghai, China). Fibrinogen, immunoglobulin G, hemoglobin, amylase, and cytochrome C were supplied by Beijing Soleibo Technology Co., Ltd. (Beijing, China). Alkaline phosphatase was purchased from Shanghai Biyuantian Biological Co., Ltd. (Shanghai, China). Sialomucin was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Two electroneutral β-CDP (one of the molecular weights was 0.3885 KD, and the other was water insoluble) were bought from Shandong Zhiyuan Biotechnology Co., Ltd. (Binzhou, China).
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2

Comprehensive Biomolecular Characterization Protocol

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Amylase (AMY), immunoglobulin G (IgG), hemoglobin (HGB), fibrinogen (FIB), cytochrome C, and thrombin were purchased from Beijing Soleibo Technology Co., Ltd. Sialomucin was provided by Shanghai yuanye Bio-Technology Co., Ltd. Myeloperoxidase (MPO), Azurocidin (AZU1), Neutrophil Elastase (ELANE), Myeloblastin (PRTN3), and T4 polynucleotide kinase (T4 PNK) were purchased from Sangon Biotech (Shanghai, China) Co., Ltd. Bovine serum albumin (BSA) was supplied by Shandong sikejie Biotechnology Co., Ltd. Alkaline phosphatase (AKP) was provided by Shanghai Biyuntian Biological Co., Ltd. N protein was offered by Sino Biological Inc. All the DNA sequences were synthesized and purified by Sangon Biotech (Shanghai, China). The specific DNA sequences are shown in Table S1. All the solutions were prepared with ultra-pure water obtained from a Millipore water purification system (>18.2 MΩ·cm). All other chemicals used in this study were of analytical grade.
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