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Eclipse a1r microscope

Manufactured by Nikon
Sourced in United States

The Eclipse A1R microscope is a high-performance confocal microscope designed for advanced imaging applications. It features a fast and sensitive laser scanning system, enabling real-time 3D imaging and high-resolution visualization of samples. The Eclipse A1R provides researchers with a robust and versatile platform for a wide range of microscopy techniques.

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2 protocols using eclipse a1r microscope

1

Quantify Mitochondrial Dynamics in Cells

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To quantify donor mitochondria internalization, fusion with host mitochondria, and mitophagy of donor and host mitochondria (per colocalization with lysosomes), stained CM were co-incubated with pHrodo Red-stained donor mitochondria. 3–10 cells/dish were imaged at baseline and after 1, 4, and 18 h co-incubation using an Eclipse A1R microscope and NIS-Elements software (Nikon Instruments Inc., Melville, NY, USA). Images were analyzed as previously described [9 (link)] using High-Content Screening Navigator (HCS) Colocalization.V4 protocol (Perkin Elmer, Waltham, MA, USA); non-internalized donor mitochondria were excluded. Baseline characteristics were evaluated using HCS Morphology.V4 protocol.
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2

Visualizing ZPFCE-NPs in BMDCs

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BMDCs labeled with ZPFCE-NPs were fixed in 4% PFA. Microscopy was performed using a confocal microscope (Nikon Eclipse A1R microscope, VIB 11, Center for the Biology of Disease, KU Leuven). Data were analyzed using the NIS Element software v4.10 (Nikon, Tokyo, Japan) and processed in Fiji software v1.49a (SC, USA).
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