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Aria mx3000

Manufactured by Agilent Technologies
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The Aria Mx3000 is a qPCR (quantitative Polymerase Chain Reaction) instrument manufactured by Agilent Technologies. It is designed to perform real-time PCR experiments for gene expression analysis, genotyping, and other applications that require precise quantification of nucleic acids.

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Lab products found in correlation

High capacity reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Rneasy plus mini kit, by Qiagen (2 mentions) Ariamx, by Agilent Technologies (2 mentions)

Spelling variants of this product

MX3000 (17 citations)

3 protocols using aria mx3000

1

Comprehensive RNA Extraction and qPCR Analysis

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Total RNA was isolated using Qiagen RNeasy plus mini kit. cDNA was synthesized with a High-Capacity Reverse Transcription kit (Thermo Fischer). qPCR was performed using Brilliant III SYBR Master Mix using Aria Mx3000 or AriaMx real time PCR machines (Agilent Genomics). Raw Ct values were normalized to geometric mean of ACTB and B2M transcripts before normalization to experimental control indicated. RT-qPCR primers are listed in Supplemental Table 2.
Schade A.E., Oser M.G., Nicholson H.E, & DeCaprio J.A. (2019). Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB.. Oncogene, 38(25), 4962-4976.
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2

Quantitative Real-Time PCR protocol

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Appropriate primers (Table S5) were used in 10 μl reactions with the Maxima 2 × SYBR Green/ROX qPCR Master Mix (Thermo Scientific) and RT‐qPCR was run on Aria Mx3000 (Agilent) with 40 cycles at 60°C for 10 s followed by 72°C for 15 s and a subsequent melting curve (65–95°C). HvUbiquitin was measured as a housekeeping gene (Schnepf et al., 2018 (link)) and foldchanges were calculated via the 2−∆∆Ct method by Livak and Schmittgen (2001 (link)).
Trutzenberg A., Engelhardt S., Weiß L, & Hückelhoven R. (2022). Barley guanine nucleotide exchange factor HvGEF14 is an activator of the susceptibility factor HvRACB and supports host cell entry by Blumeria graminis f. sp. hordei. Molecular Plant Pathology, 23(10), 1524-1537.
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3

Comprehensive RNA Extraction and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated using Qiagen RNeasy plus mini kit. cDNA was synthesized with a High-Capacity Reverse Transcription kit (Thermo Fischer). qPCR was performed using Brilliant III SYBR Master Mix using Aria Mx3000 or AriaMx real time PCR machines (Agilent Genomics). Raw Ct values were normalized to geometric mean of ACTB and B2M transcripts before normalization to experimental control indicated. RT-qPCR primers are listed in Supplemental Table 2.
Schade A.E., Oser M.G., Nicholson H.E, & DeCaprio J.A. (2019). Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB.. Oncogene, 38(25), 4962-4976.
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