Human cxcl10 ip 10 duoset elisa

Manufactured by R&D Systems

The Human CXCL10/IP-10 DuoSet ELISA is a laboratory instrument used for the quantitative measurement of human CXCL10, also known as Interferon-gamma Induced Protein 10 (IP-10), in biological samples. This ELISA kit provides the necessary components to develop a sandwich enzyme-linked immunosorbent assay (ELISA) for the analyte.

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Lab products found in correlation

Bio plex 200 system, by Bio-Rad (2 mentions) Human il 10 duoset elisa, by R&D Systems (2 mentions) Human il 6 duoset elisa, by R&D Systems (2 mentions) Substrate reagent pack, by R&D Systems (1 mentions) Bio plex pro human cytokine standard 27 plex group 1, by Bio-Rad (1 mentions) Human ccl2 mcp 1 duoset elisa, by R&D Systems (1 mentions) Bio plex cytokine assay, by Bio-Rad (1 mentions) Cyquant ldh cytotoxicity assay, by Thermo Fisher Scientific (1 mentions) Bio plex pro reagent kit 3, by Bio-Rad (1 mentions) Verikine hstm human interferon beta serum elisa kit, by PBL Assay Science (1 mentions) Verikine hs mouse ifnβ serum elisa kit, by PBL Assay Science (1 mentions) Simoa assay, by Quanterix (1 mentions) Human fabp2 1 fabp duoset elisa, by R&D Systems (1 mentions) Hyaluronan duoset elisa, by R&D Systems (1 mentions) Human cd163 duoset elisa, by R&D Systems (1 mentions) Human cd14 duoset elisa, by R&D Systems (1 mentions) Ripa buffer, by Merck Group (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions)

Close spelling variants of this product

DuoSet ELISA (635 citations) ELISAs (144 citations) CXCL10 (92 citations) DuoSets (49 citations) IP-10 (23 citations) CXCL10/IP-10 (9 citations) Human CXCL10/IP-10 DuoSet ELISA kit (3 citations) Human IP-10 Duoset ELISA (2 citations) Human ELISA DuoSet (2 citations)

10 protocols using human cxcl10 ip 10 duoset elisa

Multiplex Analysis of Serum Immune Factors

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Chemokines and other soluble factors from serum were assessed in multiplex immunoassays utilizing Luminex technology. Serum from ILP patients and from healthy donors were analyzed with a 14-plex immune checkpoint panel (Human Immuno-Oncology Checkpoint Marker Panel, Invitrogen, #EPX14A-15803-901) and a 27-plex cytokine panel (Bio-Plex™ Pro Human Cytokine Standard 27-Plex Group 1, Bio-Rad, #M500KCAF0Y) according to the manufacturers’ protocols. All measurements were performed using a Bio-Plex™ 200 System (Bio-Rad).
Soluble CXCL10, CCL2, IFN-γ, IFN-α2 and IFN-β in cell culture supernatants were measured using a CXCL10 ELISA kit (Human CXCL10/IP-10 DuoSet ELISA, R&D Systems, #DY266-05), a CCL2 ELISA kit (Human CCL2/MCP-1 DuoSet ELISA, R&D Systems, #DY279-05) and ELISA kits for IFN-γ, IFN-α2 and IFN-β (Human IFN-gamma/IFN-alpha 2/IFN-beta DuoSet ELISA, R&D Systems, #DY285B-05/#DY9345-05/#DY814-05) together with Substrate Reagent Pack (R&D Systems, #DY999) in 96-well plates (Corning™ 96-Well Half-Area Plates, Fisher Scientific, #10052511) according to manufacturer’s protocol. For samples with protein levels lower than the detection limit, 50% of the lowest detected concentration was utilized in statistical calculations.

Johansson J., Kiffin R., Aydin E., Nilsson M.S., Hellstrand K., Lindnér P., Naredi P., Olofsson Bagge R, & Martner A. (2019). Isolated limb perfusion with melphalan activates interferon-stimulated genes to induce tumor regression in patients with melanoma in-transit metastasis. Oncoimmunology, 9(1), 1684126.

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Quantifying Cytokine Responses in Human and Murine Cells

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IFNβ levels were determined using assays from PBL Assay Science: VeriKine-HSTM Human Interferon-Beta Serum ELISA Kit (#41415) for human cells’ supernatants or plasma samples, and VeriKine-HS mouse IFNβ serum ELISA kit (#42410-1) for murine plasma samples. Other cytokines (TNF, IL-1β, IL-6, MCP-1α/CCL3, IL-10, IP-10/CXCL10) for human primary cells and plasma samples and 23 cytokines for murine cells (#M60009RDPD; 23-plex Assay) were analyzed using BioPlex cytokine assays from Bio-Rad, in accordance with the instructions of the manufacturer, using the Bio-Plex Pro Reagent Kit III and Bio-Plex 200 System (Bio-Rad). For the analysis of cytokines secretion by THP-1 cells, PMA differentiated THP-1 cells were pretreated with solvents or peptides (12.5 μM) for 30 min followed by 4 h of stimulation with UP K12 LPS (100 ng/ml) and analysis of cytokine expressions in supernatants. All screens were performed in three to five biological replicates for each treatment/peptide. The following ELISA assays were used: human CXCL10/IP-10 DuoSet ELISA (DY266) and human TNF DuoSet ELISA (DY210) from R&D Systems (Biotechne) and human IL-1β ELISA Set II kit (BD OptEIA, BD Biosciences). CyQUANT LDH Cytotoxicity Assay (Thermo Fisher Scientific) was used to measure the extracellular LDH in supernatants as suggested by the manufacturer.

Nilsen K.E., Zhang B., Skjesol A., Ryan L., Vagle H., Bøe M.H., Orning P., Kim H., Bakke S.S., Elamurugan K., Mestvedt I.B., Stenvik J., Husebye H., Lien E., Espevik T, & Yurchenko M. (2023). Peptide derived from SLAMF1 prevents TLR4-mediated inflammation in vitro and in vivo. Life Science Alliance, 6(12), e202302164.

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Biomarkers of Immune Activation in HIV

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We centrally measured plasma levels of ten soluble biomarkers of all participants. sTNFRII, sCD14, sCD163, CXCL10, I-FABP, and hyaluronic acid were measured by specific ELISA (Human TNF RII/TNFRSF1B DuoSet ELISA, Human CD14 DuoSet ELISA, Human CD163 DuoSet ELISA, Human CXCL10/IP10 DuoSet ELISA, Human FABP2/I-FABP DuoSet ELISA, and Hyaluronan DuoSet ELISA, R&D Systems). IL-17, IL-6, and TNF-α were measured by single-molecule array (SiMoA) assay (Quanterix), and CRP by immunochemistry (CRP LX HS, Cobas C, integra, Roche Diagnostics). Samples with undetectable levels were attributed half the threshold value.
We measured plasma HIV RNA levels of PRIMO participants, using an ultrasensitive real-time PCR technique (GENERIC HIV, Biocentric, France) and total HIV DNA levels from whole blood samples using a real-time PCR assay (GENERIC Biocentric, France) [19] (link).
The data that support the findings of this study are available on request to the corresponding author. The data are not publicly available due to privacy restrictions.

Novelli S., Lécuroux C., Goujard C., Reynes J., Villemant A., Blum L., Essat A., Avettand-Fenoël V., Launay O., Molina J.M., Bourgeois C, & Meyer L. (2020). Persistence of monocyte activation under treatment in people followed since acute HIV-1 infection relative to participants at high or low risk of HIV infection. EBioMedicine, 62, 103129.

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CXCL10 Protein Quantification from Tissue Lysates

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Approximately 100 mg of tissue was used for CXCL10 protein quantification. Briefly, tissues were lysed with RIPA buffer (Sigma) supplemented with 1× protease inhibitor cocktail (Sigma) and 0.1-M PMSF (Sigma). Total protein quantification from lysates was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific). Quantification of lysate CXCL10 protein was determined with the Human CXCL10/IP-10 DuoSet ELISA (R&D Systems). CXCL10 levels were normalized relative to the total protein in the assay (pg/mg of total protein).

Bradburn S., McPhee J., Bagley L., Carroll M., Slevin M., Al-Shanti N., Barnouin Y., Hogrel J.Y., Pääsuke M., Gapeyeva H., Maier A., Sipilä S., Narici M., Robinson A., Mann D., Payton A., Pendleton N., Butler-Browne G, & Murgatroyd C. (2018). Dysregulation of C-X-C motif ligand 10 during aging and association with cognitive performance. Neurobiology of Aging, 63, 54-64.

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Cytokine Quantification in pDC Cultures

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After 72-hours culture, MCL supernatants were collected and spin at 10000xg for 5 minutes to remove cell debris. IL-10, TGF-β1, and TGF-β2 concentration were determined by using the Human IL-10 DuoSet^® ELISA (R&D Systems, cat. No. DY217B), Human TGF-β1 DuoSet^® ELISA (R&D Systems; cat. No. DY240), and Human TGF-β2 DuoSet^® ELISA (R&D Systems; cat. No. DY302), respectively, following the manufacturer’s instructions.
At 24 hours post-stimulation (as described in the Isolation, culture, and stimulation of human peripheral blood pDCs section), pDC supernatants were collected, as described above. The amounts of secreted IFN-α and CXCL10 were determined by using the Human IFN-α Matched Antibody Pair (eBioscience; cat. No. BMS216MST) and Human CXCL10/IP-10 DuoSet^® ELISA (R&D Systems; cat. No. DY266). The supernatants were stored at -20°C until use.

Monti M., Ferrari G., Grosso V., Missale F., Bugatti M., Cancila V., Zini S., Segala A., La Via L., Consoli F., Orlandi M., Valerio A., Tripodo C., Rossato M, & Vermi W. (2024). Impaired activation of plasmacytoid dendritic cells via toll-like receptor 7/9 and STING is mediated by melanoma-derived immunosuppressive cytokines and metabolic drift. Frontiers in Immunology, 14, 1227648.

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Quantifying Chemokine Levels with ELISA

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Chemokines were assayed using the Human CXLC9/MIG DuoSet ELISA (DY392) and Human CXCL10/IP-10 DuoSet ELISA (DY266, R&D Systems) per the manufacturer’s instructions. Optical densities were measured using a Perkin Elmer EnVision 2102 multilabel reader and analyzed using a 4 parameter logarithmic standard curve.

Liu L.Y., Strassner J.P., Refat M.A., Harris J.E, & King B.A. (2017). Repigmentation in vitiligo using the janus kinase inhibitor, tofacitinib, may require concomitant light exposure. Journal of the American Academy of Dermatology, 77(4), 675-682.e1.

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Cytokine and Chemokine Profiling

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Detection of cytokines and chemokines in the cell culture supernatant and mouse plasma was performed by Eve Technologies. The following assays were used: Human Cytokine/Chemokine 41-Plex Discovery Assay (HD41) and Mouse Cytokine/Chemokine 31-Ples Discovery Assay Array (MD31), respectively.
Confirmatory ELISAs were performed using the following kits: Human IL-6 DuoSet ELISA (R&D Systems, DY206), Human IL-8 DuoSet ELISA (R&D Systems, DY208) and Human CXCL10/IP-10 DuoSet ELISA, according to manufacturer’s instructions. The optical density at 450 nm was determined using the Multiskan FC microplate reader (Thermo Fisher Scientific) and corrected by subtracting the readings at 540 nm.

Victorelli S., Salmonowicz H., Chapman J., Martini H., Vizioli M.G., Riley J.S., Cloix C., Hall-Younger E., Machado Espindola-Netto J., Jurk D., Lagnado A.B., Sales Gomez L., Farr J.N., Saul D., Reed R., Kelly G., Eppard M., Greaves L.C., Dou Z., Pirius N., Szczepanowska K., Porritt R.A., Huang H., Huang T.Y., Mann D.A., Masuda C.A., Khosla S., Dai H., Kaufmann S.H., Zacharioudakis E., Gavathiotis E., LeBrasseur N.K., Lei X., Sainz A.G., Korolchuk V.I., Adams P.D., Shadel G.S., Tait S.W, & Passos J.F. (2023). Apoptotic stress causes mtDNA release during senescence and drives the SASP. Nature, 622(7983), 627-636.

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Quantifying CXCL10 in IFN-stimulated cells

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Human CXCL10 was detected in the culture medium of type I IFN–stimulated HuT28.11 cells with the Human CXCL10/IP-10 DuoSet ELISA (R&D systems, catalog #DY266-05). Mouse Cxcl10 was detected in the culture medium of type I IFN–stimulated CD4⁺ T cells with the Mouse Cxcl10/IP-10 DuoSet ELISA (R&D systems, catalog #DY466-05).

Hornick E.L., Wallis A.M, & Bishop G.A. (2022). TRAF3 enhances type I interferon receptor signaling in T cells by modulating the phosphatase PTPN22. Science signaling, 15(753), eabn5507.

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Cytokine Quantification in Cell Culture

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For the analysis of cytokine production in cell culture supernatants the following commercially available enzyme-linked immunosorbent assay (ELISA) kits have been used according to the manufacturers’ instructions: Human IL-22 DuoSet ELISA (DY782), Human TNFα DuoSet ELISA (DY210), Human IL-6 DuoSet ELISA (DY206), Human IL-10 DuoSet ELISA (DY217B), and Human CXCL10/IP-10 DuoSet ELISA (DY266) from R&D Systems (Minneapolis, MN) and BD OptEIA Set Human IFN-γ (555142) and BD OptEIA Set Human IL-4 (555194) from BD Biosciences. For the FLCN2 ELISA the stool was diluted in PBS at a concentration of 50 mg/mL, homogenized by vortexing for 5 minutes, and centrifuged for 10 minutes at 3000 g, and the supernatant was stored at –20°C until analyzed. FLCN2 was assayed using the Mouse Lipocalin-2/NGAL DuoSet ELISA (DY1857; R&D Systems). The samples were diluted as required and results calculated from the standard curve.

Texler B., Zollner A., Reinstadler V., Reider S.J., Macheiner S., Jelusic B., Pfister A., Watschinger C., Przysiecki N., Tilg H., Oberacher H, & Moschen A.R. (2021). Tofacitinib-Induced Modulation of Intestinal Adaptive and Innate Immunity and Factors Driving Cellular and Systemic Pharmacokinetics. Cellular and Molecular Gastroenterology and Hepatology, 13(2), 383-404.

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Profiling Cytokine and CXCL10 Levels

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Cytokine arrays were performed in accordance with the manufacturer’s protocol (R&D Systems, Proteome Profiler Array: Human Cytokine Array Kit). CXCL10 ELISA assays were performed per manufacturer’s protocol (R&D Systems, Human CXCL10/IP-10 DuoSet ELISA).

Dobish K.K., Wittorf K.J., Swenson S.A., Bean D.C., Gavile C.M., Woods N.T., Ghosal G., Hyde R.K, & Buckley S.M. (2023). FBXO21 mediated degradation of p85α regulates proliferation and survival of acute myeloid leukemia. Leukemia, 37(11), 2197-2208.

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