Anti β actin monoclonal antibody

17β-estradiol, hydroxyethylpiperazine-N’−2-ethanesulfonic acid (HEPES), fatty acid free bovine serum albumin (BSA), O-(carboxymethyl)hydroxylamine hemihydrochloride (CHH), sodium dodecyl sulfate (SDS), and all other chemicals unless specified, were from Sigma (St. Louis, MO). ICI 182, 780 (ICI), 4,4,4-(4-propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol (PPT), diarylpropionitrile (DPN) 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylet hoxy)phenol]-1H-pyrazole dihydrochloride (MPP), 4-[2-Phenyl-5,7-bis (trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3yl]phenol (PHTPP), were from Tocris (Ellisville, MO). β-cyano-L-Alanine (BCA) was from Cayman Chemical (Ann Arbor, MI). Anti-β-actin monoclonal antibody was from Ambion (Austin, TX). Fetal bovine serum (FBS) was from Lonza (Walkersville, MD). Monoclonal antibodies against ERα and ERβ were from Fisher Scientific (Pittsburgh, PA). Antibodies against CBS, CSE, α-smooth muscle actin (α-SMA), eNOS, and caveolin-1 were from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). DMEM and M199, Platinum Taq DNA polymerase, and Alexa⁴⁸⁸ and Alexa⁵⁸⁸-labeled immunoglobulin G (IgG) were from Invitrogen (Carlsbad, CA).

Anti-Flag and FITC-labeled anti-Flag antibodies, bovine serum albumin (BSA), 4-(2-hydroxyethyl)-1-piperazineethane-sulfonic acid (HEPES), N-nitro-L-arginine-methylester (L-NAME), N-nitro-D-arginine-methylester (D-NAME), and all other chemicals unless specified, were from Sigma (St Louis, MO). N-[6-(biotinamido) hexyl]-3'-(2'-pyridyldithio) propionamide (biotin-HPDP) was from Thermo scientific (Rockford, IL). S-nitrosoglutathione (GSNO) was from Cayman (Ann Arbor, MI). Anti-CFL1 antibody was from Abcam (San Francisco, CA). Anti-β-actin monoclonal antibody was from Ambion (Austin, TX). Anti-eNOS antibody was from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). Prolong Gold antifade reagent with 4',6-diamidino-2-phenylindole, MCDB131 and M199 were from Invitrogen (Carlsbad, CA).