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N1630

Manufactured by Merck Group
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N1630 is a laboratory equipment product from Merck Group. It is designed for general use in laboratory settings. The core function of N1630 is to provide a reliable and efficient solution for various laboratory tasks. Further details about its specific intended use or features are not available at this time.

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Lab products found in correlation

Espectra max 2, by Molecular Devices (2 mentions) S7903, by Merck Group (2 mentions) M1000, by Xenotech (1 mentions) N5514, by Merck Group (1 mentions)

4 protocols using n1630

1

NADPH Oxidase Activity Assay

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Nicotinamide adenine dinucleotide phosphate oxidase (NADPH) oxidase was determined by the rate of NADPH consumption assessed by measuring the decline in absorbance (340 nm) every 10 min, using a plate reader spectrophotometer (Espectra Max 2, Molecular Devices) (Wei et al., 2006 (link)). For the assay, we used a 50 mM phosphate buffer containing EDTA (2 mM, Nuclear, 311737), sucrose (150 mM, Sigma–Aldrich Corporation, S7903), NADPH (1.3 mM, Sigma–Aldrich Corporation, N1630), and 10 μL of sample.
Araujo G.S., Silva T.O., Guerra G.M., Izaias J.E., Rocha H.M., Faria D., Rocha N.G., Dalmazo A.L., Araujo A., Marciano Consolim-Colombo F., de Angelis K., Irigoyen M.C, & Sales A.R. (2022). Effects of Postprandial Lipemia Combined With Disturbed Blood Flow on the Flow-Mediated Dilation, Oxidative Stress, and Endothelial Microvesicles in Healthy Subjects. Frontiers in Physiology, 13, 812942.
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2

Intrinsic Clearance Determination in Mouse Microsomes

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Intrinsic clearance (CLi) values were determined in mouse liver microsomes (M1000, XenoTech). Test compounds (final concentration 0.5 μM) were incubated at 37 °C for 45 min in 50 mM potassium phosphate buffer (pH 7.4) containing 0.5 mg microsomal protein per ml. The reaction was started by addition of cofactor NADPH (N1630, Sigma) at 1 mM final concentration. The final concentration of organic solvent (DMSO) was limited to 0.25% of the final volume. At 0, 5, 15, 30, and 45 min, an aliquot (100 μl) was taken, quenched with acetonitrile containing an appropriate internal standard, and analysed by HPLC-MS/MS (Applied Biosystems). CLi was determined from the first-order elimination constant by nonlinear regression, corrected for the volume of the incubation and assuming 48 microsomal mouse protein per g liver. Values for CLi were expressed as ml min−1 per g liver.
Miguel-Blanco C., Molina I., Bardera A.I., Díaz B., de las Heras L., Lozano S., González C., Rodrigues J., Delves M.J., Ruecker A., Colmenarejo G., Viera S., Martínez-Martínez M.S., Fernández E., Baum J., Sinden R.E, & Herreros E. (2017). Hundreds of dual-stage antimalarial molecules discovered by a functional gametocyte screen. Nature Communications, 8, 15160.
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3

Whole-Mount NADPH Diaphorase Staining in Colon

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Whole-mount staining for NADPH diaphorase was performed using previously described protocols [27 (link)]. Briefly, the colons were collected, washed and then filled with PBS, tying the ends with suture thread. Tissues were subsequently fixed with 4% PFA for 15–30 min and rinsed with PBS (four times, 5 min each). After that, tissues were immersed in 0.3% Triton X-100 (diluted in PBS) for 20 min, followed by incubation in the NADPH diaphorase staining solution containing 0.1% β-NADPH (Sigma, catalog N1630), 0.05% Nitro blue Tetrazolium (Sigma, catalog N5514), and 0.3% Triton X-100. The reaction was performed at 37 °C and stopped when nerve elements were detected. The colons were washed, post-fixed with 4% PFA, and cut along the mesenteric attachment. The mucosal layer was peeled off under a dissection microscope and the specimens were visualized under a light microscope.
Wang H., Jing R., Trexler C., Li Y., Tang H., Pan Z., Zhu S., Zhao B., Fang X., Liu J., Chen J, & Ouyang K. (2018). Deletion of IP3R1 by Pdgfrb-Cre in mice results in intestinal pseudo-obstruction and lethality. Journal of gastroenterology, 54(5), 407-418.
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4

NADPH Oxidase Activity Assay

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Nicotinamide adenine dinucleotide phosphate oxidase (NADPH) oxidase was determined by the rate of NADPH consumption assessed by measuring the decline in absorbance (340 nm) every 10 min, using a plate reader spectrophotometer (Espectra Max 2, Molecular Devices) (Wei et al., 2006 (link)). For the assay, we used a 50 mM phosphate buffer containing EDTA (2 mM, Nuclear, 311737), sucrose (150 mM, Sigma–Aldrich Corporation, S7903), NADPH (1.3 mM, Sigma–Aldrich Corporation, N1630) and 10 μL of sample.
Fetter C., Marques J.R., de Souza L.A., Dartora D.R., Eibel B., Boll L.F., Goldmeier S.N., Dias D., De Angelis K, & Irigoyen M.C. (2020). Additional Improvement of Respiratory Technique on Vascular Function in Hypertensive Postmenopausal Women Following Yoga or Stretching Video Classes: The YOGINI Study. Frontiers in Physiology, 11, 898.
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