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Accumet

Manufactured by Thermo Fisher Scientific
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Accumet is a line of laboratory equipment manufactured by Thermo Fisher Scientific. The core function of Accumet products is to measure and monitor various parameters in laboratory settings, such as pH, conductivity, and dissolved oxygen levels. Accumet offers a range of instruments, electrodes, and accessories to support these measurement tasks.

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Lab products found in correlation

Nucleocounter nc 200, by ChemoMetec (1 mentions) Evolution 201, by Thermo Fisher Scientific (1 mentions) Jem 2100, by JEOL (1 mentions) Litesizer 500, by Anton Paar (1 mentions) Gf f filter, by Cytiva (1 mentions) 1 n hcl, by Merck Group (1 mentions) Durapore membrane, by Merck Group (1 mentions) Vented erlenmeyer shake flasks, by Corning (1 mentions) 1n naoh, by Merck Group (1 mentions) Rtesp probe, by Bruker (1 mentions) Smartlab x ray diffractometer, by Rigaku (1 mentions) Mcr 92, by Anton Paar (1 mentions)

14 protocols using accumet

1

Quantification of Malate in EBC Extract

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The pH of the EBC extract was measured directly in the fluid obtained with a pH micro-electrode PerpHecT Ross microcombination pH electrode (8220BNWP model, Thermo-Fisher Scientific) connected to a pH meter (Accumet, Fisher Scientific).
Malate was quantified enzymatically by a coupled enzyme assay according to Hohorst [56 ]. The reaction medium contained 50 mM glycylglycine (pH 10), 30 mM L-glutamate, 3 mM NAD+, I U of glutamate oxaloacetate transaminase (GOT, Sigma-Aldrich), 10 U of L-malate dehydrogenase (MDH, Sigma-Aldrich). Malate concentrations were obtained by calculating the difference in the absorbance at 340 nm before and after 20 min incubation at RT. Measurements of pH and malate were made on four independent samples for each time and treatment, and the results for malate were expressed as μmol malate ml−1 of EBC extract.
Barkla B.J., Vera-Estrella R, & Raymond C. (2016). Single-cell-type quantitative proteomic and ionomic analysis of epidermal bladder cells from the halophyte model plant Mesembryanthemum crystallinum to identify salt-responsive proteins. BMC Plant Biology, 16, 110.
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2

Bioreactor Sampling and Monitoring

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Each day a 20–30 mL sample was taken from the bioreactor and stored in a sterile 250 mL bottle to ensure a representative sample of microcarriers was taken from the vessel. From this sample 2 × 3 mL samples were taken for counting using a NucleoCounter NC-200 (ChemoMetec) using the A&B assay. Each sample was counted twice. For visualization, cell samples were prepared using the same method as with the 0.1 and 0.5 L bioreactors. Finally, a 3 mL sample was also taken each day for off-line pH samples using a benchtop pH probe (Fisher Scientific, Accumet, AB150).
Roberts E.L., Abraham B.D., Dang T., Gysel E., Mehrpouyan S., Alizadeh A.H., Koch T.G, & Kallos M.S. (2023). Computer controlled expansion of equine cord blood mesenchymal stromal cells on microcarriers in 3 L vertical-wheel® bioreactors. Frontiers in Bioengineering and Biotechnology, 11, 1250077.
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3

Kinetics of NaHCO3 Synthesis

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Using a silver ion selective electrode (Fisher Scientific accumet), the kinetics of the NaHCO3 synthesis method were explored at temperature profile 1 (60 °C heated to 80 °C) and temperature profile 2 (80 °C from the start) by measuring the decrease in ionic silver present in the solution (assumed to correspond to formation of particulate silver on the textile). It is important to note that, as the ISE only measures ionic silver, any release of metallic silver from the textile during synthesis would not be identified. A no-textile control was analyzed using ISE at both temperature profiles to correct for changes to ISE response as a function of temperature. A six-point external calibration curve at the reaction temperature was used to quantify the ionic silver.
Patch D., O’Connor N., Meira D., Scott J., Koch I, & Weber K. (2023). Parsimonious methodology for synthesis of silver and copper functionalized cellulose. Cellulose (London, England), 30(6), 3455-3472.
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4

Comprehensive Characterization of Nanoparticles

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The particles’ hydrodynamic size distribution was assessed using a Litesizer 500 (Anton Paar, Austria) at a scattering angle of 90° at 28 °C to get a basic feel of their average size and particle size distribution (hydrated sphere). UV/Vis spectroscopy was used to determine the optical properties of the materials, with a UV/Vis spectrophotometer (Thermo Scientific Evolution 201, US) operating in between 200- and 800-nm wavelength range and a quartz cuvette with a path length. Powder X-ray diffraction was used to determine the solid phase of nanoparticles (PXRD, Explorer, GNR Italy). To expose the particles, monochromatized Cu K radiation (λ = 1.54) was employed. For size and structural morphology, a JEM-2100 (JEOL, Japan) transmission electron microscope (TEM) with energy-dispersive X-ray spectroscopy (EDS) for element composition and selected area electron diffraction (SAED) for crystalline phase identification was utilised. Functional groups on the surface were analysed using Fourier-transform infrared spectroscopy (FTIR, alphaT, Bruker, Germany), with scanning in the range of 3500–500 cm−1. A centrifuge (Remi R-4C) was used to determine the centrifugation force. A pH metre was used to regulate the pH of the liquids (Fisher Scientific, Accumet).
Ghosh N., Sen S., Biswas G., Saxena A, & Haldar P.K. (2023). Adsorption and Desorption Study of Reusable Magnetic Iron Oxide Nanoparticles Modified with Justicia adhatoda Leaf Extract for the Removal of Textile Dye and Antibiotic. Water, Air, and Soil Pollution, 234(3), 202.
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5

Soil Nutrient and Moisture Analysis

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For each soil sample, pH, percent soil moisture, nitrate (NO3−), and ammonium (NH4+) concentrations were measured on field-cooled soils within 5 days of collection. Large plant roots were removed from all samples prior to taking any measurements. The pH was measured by mixing 5 g of field fresh soil with 10 mL of distilled deionized (DDI) water with a stir bar and recording stable pH using a laboratory meter (Fisher Accumet). Percent soil moisture was determined by placing 10 g of field fresh soil into an aluminum tin and determining the change in mass of soil before and after drying at 65 °C for 1 week. To measure NO3− and NH4+ concentrations, 10 g of fresh soil were shaken at 150 rpm in acid-washed centrifuge tubes with 50 mL of 2 M KCl for 1 h, then centrifuged at 3400 rpm for 5 min and finally filtered through a GF/F filter (Whatman). NO3− and NH4+ concentrations were measured from extracts using 96-well plate protocols [49 (link)].
Pearce D.S., Hoover B.A., Jennings S., Nevitt G.A, & Docherty K.M. (2017). Morphological and genetic factors shape the microbiome of a seabird species (Oceanodroma leucorhoa) more than environmental and social factors. Microbiome, 5, 146.
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6

Kinetic Study of Sulfide Oxidation

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Samples (250 mL) of SAF-MBR effluent were spiked with sulfides. Hydrochloric acid or sodium hydroxide was used to adjust sample pH; pH was measured using a pH probe (Fisher Accumet). Once the target pH was reached, the 250 mL samples were aliquoted into 25 mL vials and capped headspace-free. Control experiments indicated that sulfide concentrations in each vial were within ~5% of each other, and sulfide concentrations did not decrease over the ~2 h time frame of the kinetics experiments. To initiate the reaction, H2O2 was injected into samples using a syringe. At each timepoint, 25 mL vials were sacrificed for immediate absorbance and total sulfide analysis. The remainder of each 25 mL vial was treated with 5 mM ZnCl2 to precipitate ZnS; for low pH samples, NaOH was added to promote ZnS precipitation. H2O2 and sulfate concentrations were measured by colorimetric methods immediately after ZnCl2 treatment. Samples were then treated with 2 mg/L catalase (250 units/mg) to degrade residual H2O2, and were saved for sulfite and thiosulfate analysis by ion chromatography. Control experiments showed that ZnCl2 did not affect H2O2, sulfate, sulfite, and thiosulfate concentrations, and that quenching of H2O2 by catalase did not affect the analysis of sulfate, sulfite, and thiosulfate by ion chromatography. Experiments were conducted in duplicate.
Szczuka A., Berglund-Brown J.P., MacDonald J.A, & Mitch W.A. (2021). Control of sulfides and coliphage MS2 using hydrogen peroxide and UV disinfection for non-potable reuse of pilot-scale anaerobic membrane bioreactor effluent. Water Research X, 11, 100097.
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7

Optimization of Baculovirus-Insect Cell Expression of Chikungunya Virus

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Serum-free Sf-900II medium (Gibco) was obtained at a pH of 6.3 and was adjusted to different target pH levels: 1 N HCl (Sigma-Aldrich) was used to reduce pH to 6.0, and 1 N NaOH (Sigma-Aldrich) was used to increase pH to 6.6–6.8. Growth medium pH was measured using a calibrated pH meter and probe (Fisher Scientific Accumet), and the pH-adjusted medium was sterile filtered through a 0.2 μm Durapore membrane (EMD Millipore). Sf21 cells were centrifuged at 200× g, spent Sf-900II media was fully aspirated, and the cells were re-suspended in pH 6.0–6.8 formulations of Sf-900II. Re-suspended Sf21 cultures (at 3×106 viable cells/mL) were inoculated with AcMNPV-CHIKV37997 in Sf-900II media at an MOI of 1 pfu per viable cell. 150 mL cultures were inoculated in 500-mL vented Erlenmeyer shake flasks (Corning). Inoculated cultures were incubated at 27°C in a shaking incubator (Kuhner) set to 80 RPM and a 2″ shaking diameter. Cell suspension samples were removed 72 hours post-infection for immunofluorescence flow cytometry. Harvest samples were removed 96 hours post-infection, centrifuged to remove cells, and submitted to qELISA analysis. Statistical analysis was performed using Minitab 16 software (Minitab).
Wagner J.M., Pajerowski J.D., Daniels C.L., McHugh P.M., Flynn J.A., Balliet J.W., Casimiro D.R, & Subramanian S. (2014). Enhanced Production of Chikungunya Virus-Like Particles Using a High-pH Adapted Spodoptera frugiperda Insect Cell Line. PLoS ONE, 9(4), e94401.
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8

Measurement of Biofilm pH Levels

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A pH meter (Accumet, Fisher Scientific, Pittsburgh, PA, USA) was used to measure the pH of the pooled samples of every group. The supernatant containing planktonic cells on top of the biofilm was removed before light irradiation for pH measurement. The pH was measured on the first day before the treatment and on the fifth or final day of the treatment regimen.
Felix Gomez G.G., Lippert F., Ando M., Zandona A.F., Eckert G.J, & Gregory R.L. (2018). Effect of Violet-Blue Light on Streptococcus mutans-Induced Enamel Demineralization. Dentistry Journal, 6(2), 6.
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9

Electrochemical Deposition of Fe(OH)2/Ni(OH)2 Nanoparticles

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The Fe(OH)2/Ni(OH)2 nanoparticles were prepared by a facile two-step electrochemical deposition technique. The deposition was conducted in a three-electrode setup, with nanoporous Mo:BiVO4 as the working electrode, saturated calomel electrode (Accumet, Fisher Scientific) as the reference electrode, and platinum wire as the counter electrode in a freshly prepared solution of 50 mM (NH4)2Fe(SO4)2 and 50 mM Ni(NO3)2. The electrodeposition of Fe(OH)2/Ni(OH)2 was first applied at −0.5 V in a 50 mM (NH4)2Fe(SO4)2 aqueous solution for 2 min and was then applied at −0.45 V in a 50 mM Ni(NO3)2 aqueous solution for another 2 min. After electrodeposition, the electrode was rinsed with water several times to remove the residue solution and then air-dried at room temperature. When irradiated by sunlight, the photogenerated holes from Mo:BiVO4 converts Fe(OH)2/Ni(OH)2 into FeOOH/NiOOH [Fe(Ni)OOH], which will promote the OER.
Qiu Y., Liu W., Chen W., Chen W., Zhou G., Hsu P.C., Zhang R., Liang Z., Fan S., Zhang Y, & Cui Y. (2016). Efficient solar-driven water splitting by nanocone BiVO4-perovskite tandem cells. Science Advances, 2(6), e1501764.
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10

Chikungunya Virus-Like Particle Production

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Serum-free Sf21 and SfBasic cultures at 3×106 viable cells/mL were inoculated with AcMNPV-CHIKV37997 in Sf-900II media at an MOI of 0.1 pfu per viable cell. 35 mL cultures were inoculated in 125-mL vented Erlenmeyer shake flasks (Corning), and 300 mL cultures were inoculated in 2-L vented Erlenmeyer shake flasks (Corning). Inoculated cultures were incubated for 24 hours at 27°C in a shaking incubator (Kuhner) set to 80 RPM and a 2″ shaking diameter to initiate the infection. Infected cells were centrifuged at 200× g, the spent Sf-900II media was fully aspirated, and cells were resuspended in pH 7.4 Sf-900II-BES-MISS for VLP production. Culture pH was maintained between 7.0–7.4 by monitoring the pH of samples via calibrated benchtop pH meter and probe (Fisher Scientific Accumet) and aseptically adding sterile 1 N NaOH at a rate of 15 μL of 1 N NaOH/pH unit/mL of culture. Samples were removed at 72 and 96 hours post-infection, centrifuged to remove cells, and submitted for qELISA analysis. Data points without explicit time-point indications are 96 hour post-infection harvest samples. Statistical analysis was performed using Minitab 16 software (Minitab).
Wagner J.M., Pajerowski J.D., Daniels C.L., McHugh P.M., Flynn J.A., Balliet J.W., Casimiro D.R, & Subramanian S. (2014). Enhanced Production of Chikungunya Virus-Like Particles Using a High-pH Adapted Spodoptera frugiperda Insect Cell Line. PLoS ONE, 9(4), e94401.
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