Giemsa azur eosine methylene blue solution

ActinRed^TM 555, ActinGreen^TM 488, NucBlue Hoechst 33342 were from Molecular Probes (Oregon, OR, USA). AnnexinV-FITC was from BD bioscience (San Jose, CA, USA). DRAQ7^TM was from Biostatus (Shepshed, UK). Trichostatin A (TSA) was from Sigma-Aldrich (Steinheim, Germany). May-Grünwald Eosine-methylene blue solution (product number: HX68862424) and Giemsa Azur-Eosine-methylene blue solution (product number: HX128350) were from Merck KGaA (Darmstadt, Germany). SYBR GreenER SuperMix and Rox reference dye were from Invitrogen (Carlsbad, CA, USA).

PD98059 (MEK inhibitor) was obtained from Sigma-Aldrich (Product number P215). U0126 (CAS109511-58-2) (MEK inhibitor) was purchased from EMD Millipore (Burlington, MA, USA). For both inhibitors, stock solutions were prepared in DMSO. The following antibodies were used: anti-ERK1/2, anti-P-ERK1/2, anti-P-JNK and anti-P-P38 [Cell Signaling Technology, anti-actin (I-19); Santa Cruz Biotechnology, Dallas, TX, USA], and anti-Mcpt6 antiserum (raised in rabbits). Monocloncal IgE anti-Dinitrophenyl (IgE anti-DNP) antibody was from Sigma (Stockholm, Sweden; product number: D8406). CellTiter-Blue Cell Viablity Assay was purchased from Promega-Invitrogen (Madison, WI, USA). The chromogenic substrate S-2288 was obtained from Chromogenix (Milan, Italy). May-Grünwald Eosine-methylene blue solution (product number: HX68862424) and Giemsa Azur-Eosine-methylene blue solution (product number: HX128350) were from Merck KGaA (Darmstadt, Germany). SYBR GreenER SuperMix and Rox reference dye were from Invitrogen (Carlsbad, CA, USA). Pierce phosphatase inhibitor was obtained from Thermo Fisher Scientific (Waltham, MA, USA) and protease inhibitor cocktail was from Roche Diagnostics.