Dulbecco s mem dmem

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Dulbecco's Modified Eagle's Medium (DMEM) is a cell culture medium commonly used to support the growth of a variety of mammalian cell lines. It provides a balanced salt solution, amino acids, vitamins, and other nutrients required for cell proliferation.

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Dulbecco’s MEM (36 citations) S-MEM (19 citations) DMEM/MEM (3 citations)

6 protocols using dulbecco s mem dmem

SPION Effects on HPV+ and HPV- HNSCC

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Four different HNSCC, primary human fibroblasts and healthy keratinocytes were used to study the influence of different SPIONs systems in combination with ionizing radiation. The HNSCC Cal33 and HSC4 are HPV-16 negative (HPV-), whereas UM-SCC-47 and UD-SCC-2 are HPV-16 positive (HPV+) [33 (link)]. Samples were obtained courtesy of Dr. Thorsten Rieckmann from the University of Medical Centre Hamburg-Eppendorf, Germany. Primary human skin fibroblasts SBLF9 were derived from a healthy young Caucasian men. Healthy keratinocytes HaCAT were derived from healthy individuals and subsequently cultured. They were obtained from the Department of Ophtalmology, University Clinic Erlangen [34 (link)].
Fibroblasts were grown in F-12 medium (Gibco, Waltham, MA, USA) with 15% fetal bovine serum (FBS, Biochrom, Berlin, Germany), 5% penicillin-streptomycin (Gibco, Waltham, MA, USA), and 5% non-essential amino acids (NEA, Biochrom, Berlin, Germany). Keratinocytes were cultured in Dulbecco’s MEM (DMEM, Gibco, Waltham, MA, USA) with 4.5 g/L, 10% FBS and 1% penicillin-streptomycin. HNSCC were cultured in Dulbecco’s MEM (DMEM, Gibco, Waltham, MA, USA) supplemented with 10% FBS and 1% penicillin-streptomycin. All cells were incubated at a constant temperature of 37 °C and in a humidified atmosphere with 5% CO₂.

Schreiber C., Franzen T., Hildebrand L., Stein R., Friedrich B., Tietze R., Fietkau R, & Distel L.V. (2022). Effect of Citrate- and Gold-Stabilized Superparamagnetic Iron Oxide Nanoparticles on Head and Neck Tumor Cell Lines during Combination Therapy with Ionizing Radiation. Bioengineering, 9(12), 806.

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Genotoxicity Assay with V79 and SHE Cells

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V79 cells (lung fibroblast from Chinese hamster, ATCC, USA, reference CCL-93) were selected for this study as they are one of the cell models recommended in OCDE guideline number 487 for use in the in vitro micronucleus assay. Cells were grown in Dulbecco's MEM (DMEM; Invitrogen, France), supplemented with 10% fetal calf serum (Dutscher, France) and 0.5% Penicillin/Streptomycin (5000 U-5000 μg/mL, Invitrogen, France). Cells were incubated at 37°C with 10% CO₂, as recommended by the supplier for optimal culture with our medium.
Syrian hamster embryo (SHE) cell cultures were used as they are normal diploid cells, nongenetically modified, metabolically competent, and p53 effective and there is no known difference with those constituting the organism where they come from. They have been demonstrated to be suitable for genotoxicity assays [37 (link), 38 (link)]. Cells were established from individual 13-day gestation foetuses (inbred colony, INRS, France). The culture medium used was Dulbecco's MEM (DMEM; Invitrogen, France), supplemented with 17% fetal calf serum (Dutscher, France) and 0.5% Penicillin/Streptomycin (5000 U-5000 μg/mL, Invitrogen, France). Cells were incubated at 37°C and 10% CO₂.

Darne C., Terzetti F., Coulais C., Fontana C., Binet S., Gaté L, & Guichard Y. (2014). Cytotoxicity and Genotoxicity of Panel of Single- and Multiwalled Carbon Nanotubes: In Vitro Effects on Normal Syrian Hamster Embryo and Immortalized V79 Hamster Lung Cells. Journal of Toxicology, 2014, 872195.

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Cultured Human Glioma and Hepatocyte Cells

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The human glioma cell lines U87MG and LN-229 and LO2 hepatocytes were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). U87MG cells were cultured in modified Eagle’s medium (MEM) (HyClone, South Logan, UT, USA) with 10% fetal bovine serum (FBS; Sigma-Aldrich). LN-229 cells were cultured in Dulbecco’s MEM (DMEM) (Gibco) with 5% FBS. The LO2 hepatocytes were cultured in RPMI-1640 medium (Gibco) with 10% FBS. Penicillin (100 U/ml) and streptomycin (100 μg/ml) (PS) (Gibco) were also added to the three types of media. Primary glioma cells were isolated from fresh glioma tissues obtained from 20 patients undergoing surgical resection (Department of Neurosurgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, P.R. China), with a confirmed diagnosis of glioma following postoperative pathological examination. The tissue acquisition was approved by the ethics committee of the hospital, and all the patients signed informed consent forms. All the cells were cultured in a humidified atmosphere containing 5% CO₂ at 37°C.

Liu J., Zhang Y., Sun S., Zhang G., Jiang K., Sun P., Zhang Y., Yao B., Sui R., Chen Y., Guo X., Tang T., Shi J., Liang H, & Piao H. (2019). Bufalin Induces Apoptosis and Improves the Sensitivity of Human Glioma Stem-Like Cells to Temozolamide. Oncology Research, 27(4), 475-486.

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Cell Culture Protocols for Immunology Research

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THP-1 human monocyte cells, Jurkat T cells (ATCC, Manassas, VA, USA) were cultured in RPMI media (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) and 1% penicillin and streptomycin (pen/strep). J774 mouse macrophages (ATCC, Manassas, VA, USA) were cultured in Dulbecco’s MEM (DMEM, Gibco, Grand Island, NY, USA) supplemented with 10% FBS, 1% pen/strep. Normal human lung fibroblasts (NHLFs) were obtained from Lonza, Walkersville, MD, USA and cultured in Fibroblast basal Medium-2 (Lonza, Walkersville, MD, USA) with supplements (FGM-2 bullet kit, Lonza, Walkersville, MD, USA). The NHLFs were passaged and used till passage 4. Human Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy volunteers from New York Blood Center upon obtaining informed consent and after Institutional Review Board (IRB) approval. were isolated by Ficoll separation and were maintained in RPMI-1640 medium (Gibco, Grand Island, NY, USA) with 10% FBS (Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (Gibco, Grand Island, NY, USA) prior to the experiment. All the cells were incubated in 5% CO₂ supply at 37 °C.

Mukundan S., Singh P., Shah A., Kumar R., O’Neill K.C., Carter C.L., Russell D.G., Subbian S, & Parekkadan B. (2021). In Vitro Miniaturized Tuberculosis Spheroid Model. Biomedicines, 9(9), 1209.

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HNSCC and Fibroblast Cell Culture

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Cal33, HSC4, UM-SCC-47, and UD-SCC-2 are HNSCC cells and were kindly provided by Dr. Thorsten Rieckmann (University Medical Center Hamburg-Eppendorf, Germany). As described before [5 (link),9 (link),10 (link)], Cal33 and HSC4 are HPV-negative, whereas UM-SCC-47 and UD-SCC-2 contain sequences of the most common high-risk type HPV-16. Human skin fibroblasts SBLF7 and SBLF9 were derived from healthy caucasian males and cultured subsequently. HNSCC were cultured in Dulbecco’s MEM (DMEM; Gibco, Waltham, MA, USA) supplemented with 10% FBS (Biochrom, Berlin, Germany) and 5% penicillin-streptomycin (Gibco, Waltham, MA, USA). Fibroblasts were cultured in F-12 (Gibco, Waltham, MA, USA) supplemented with 15% FBS (Biochrom, Berlin, Germany), 5% penicillin-streptomycin (Gibco, Waltham, MA, USA), and 5% NEA (Biochrom, Berlin, Germany). All cell cultures were grown at 37 °C and 5% CO₂ in a humidified atmosphere.

Dobler C., Jost T., Hecht M., Fietkau R, & Distel L. (2020). Senescence Induction by Combined Ionizing Radiation and DNA Damage Response Inhibitors in Head and Neck Squamous Cell Carcinoma Cells. Cells, 9(9), 2012.

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Engineered Colorectal Cancer Cell Lines

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Human colorectal cancer cell lines were cultured in Dulbecco's MEM (DMEM; Invitrogen) supplemented with 10% heat-inactivated fetal calf serum (FBS; HyClone). Cell lines (RKO, SW480, and HCT116) were purchased from ATCC (LGC standards). No authentication was done by the authors. Full-length FOXE1 cDNA was subcloned into the pIRES-neo3 expression vector (Clontech Laboratories Inc.). GFP-SYNE1 and GFP-SYNE1-KASH (Klarischt) were kindly provided by Dr. Zhang (Department of Medicine, Cambridge, United Kingdom).
HCT116 cells were transfected with the Nucleofector Kit V (Amaxa Biosystems) and RKO and SW480 cells were transfected using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's protocol. HCT116, RKO, and SW480 cells were transfected with a control construct (empty vector) or FOXE1-pIRESneo3, GFP-SYNE1, and GFP-SYNE1-KASH, selected for 10 days with G418 (HCT116 and SW480 400 µg/mL; RKO 1 mg/mL).

Melotte V., Yi J.M., Lentjes M.H., Smits K.M., Van Neste L., Niessen H.E., Wouters K.A., Louwagie J., Schuebel K.E., Herman J.G., Baylin S.B., van Criekinge W., Meijer G.A., Ahuja N, & van Engeland M. (2014). Spectrin Repeat Containing Nuclear Envelope 1 and Forkhead Box Protein E1 Are Promising Markers for the Detection of Colorectal Cancer in Blood. Cancer prevention research (Philadelphia, Pa.), 8(2), 157-164.

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