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4 protocols using heptanesulfonic acid

1

Chromatography Solvent Preparation

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Methanol, acetonitrile, acetic acid, nitric acid, heptanesulfonic acid, hydrochlorid acid, peryodic acid, and peroxide hidrogene were purchased from Merck (Merck, Darmstadt, Germany). Deionizated water (<18 MΩ cm resistivity) was obtained from a MicroPure water purification system (Thermo scientific, Asheville, NC, USA). Chromatographic solvents were filtered through a membrane filter 0.45 µm from Merck (Merck Millipore Ltd., Cork, Ireland).
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2

Neurochemical Analysis of Biological Samples

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Pregnant mare's serum gonadotropin (PMSG), human chorionic gonadotropin (HCG), nasteride, β-Cyclodextrin, estradiol, progesterone, pentylenetetrazole, ferulic acid, dopamine, serotonin and methanol (HPLC grade) were obtained from Sigma (USA), Heptane sulfonic acid (Merck, India), perchloric acid and tartaric acid (Spectrochem, Mumbai), norepinephrine (Troikaa Pharmaceuticals, India). Sodium-Lglutamate (S D Fine-Chem limited, India), trichloroacetic acid and pyridoxal-5-phosphate (Sigma Chemical Co., St. Louis MO, USA), ninhydrin and gamma-aminobutyric acid (GABA) (Sigma Chemical Co., St. Louis MO, USA), triton X-100 (scintillation grade) (Loba Chemie) were also used in the study. All other chemical reagents were of analytical grade.
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3

Monoamine Regulation Protocol

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Pentylenetetrazole and serotonin was procured from Sigma-Aldrich, Co. (St. Louis, MO, USA). HPLC-grade methanol and heptane sulfonic acid was procured from Merck Specialties (Mumbai, India). Norepinephrine was obtained as a gift sample from Troikaa Pharmaceuticals Ltd. (Dehradun, India). Perchloric acid was purchased from Spectrochem (Mumbai, India). Imipramine hydrochloride, fluoxetine hydrochloride, venlafaxine hydrochloride and mirtazapine were obtained as gift samples from Parasol Laboratories, Baddi, Himachal Pradesh (India).
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4

Oxidative Stress Biomarker Assay Protocol

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Acetic acid, acetonitrile, heptanesulfonic acid, hydrochloric acid, periodic acid methanol, nitric acid, and hydrogen peroxide were purchased from Merck (Merck, Darmstadt, Germany). Deionized water was obtained from a MicroPure water purification system (Thermo Scientific, Asheville, NC, USA). Chromatographic solvents were filtered through a membrane filter 0.45 μm from Merck (Merck Millipore Ltd., Cork, Ireland). The SOD, CAT, GPx, and GR assay kits were purchased from Sigma-Aldrich (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany).
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