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N n diisopropylethylamine dipea triisopropylsilane tips 1 2 ethanedithiol edt

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N,N-diisopropylethylamine (DIPEA) is a tertiary amine compound used as a base and nucleophile in organic synthesis. Triisopropylsilane (TIPS) is a silane compound used as a protecting group and reducing agent in organic chemistry. 1,2-ethanedithiol (EDT) is a dithiol compound used as a chelating agent and a reducing agent in organic synthesis.

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3 protocols using n n diisopropylethylamine dipea triisopropylsilane tips 1 2 ethanedithiol edt

1

Peptide Synthesis and Microbial Assays

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Mueller-Hinton, Agar SPC, Mueller Hinton Broth (MHB), E. coli ATCC 11775, S. maltophilia ATCC 13636, and S. enteritidis ATCC 13076 were obtained from ATCC (Manassas, VA, USA). BLF, N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), 4-methylpiperidine, pyridine, and ninhydrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rink amide resin, Fmoc-amino acids, 6-chloro-1-hydroxy-benzotriazole (6-Cl-HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropylalcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). All reagents were used without further purification.
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2

Peptide Synthesis Using Standard Reagents

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Mueller-Hinton, Agar SPC, Mueller Hinton Broth (MHB), E. coli ATCC 11775 and E. coli ATCC 25,922 were obtained from ATCC (Manassas, VA, USA), Fetal Bovine Serum (FBS) was obtained of Gibco. N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), 4-methylpiperidine, pyridine, and ninhydrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rink amide resin, Fmoc-amino acids, 6-chloro-1-hydroxy-benzotriazole (6-Cl-HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropylalcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). All reagents were used without further purification.
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3

Cell Viability and Apoptosis Assay Protocol

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MCF-7 and CRL-3271 cells were obtained from ATCC (Manassas, VA, USA), fetal bovine serum (FBS) was obtained of Gibco. MitoProbe™ JC-1 Assay Kit (Termofisher; M34152), Calcium Assay Kit (BD Biosciences; 640176), 7AAD and Annexin V (BD Biosciences; 559763) N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), 4-methylpiperidine, pyridine, and ninhydrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rink amide resin, Fmoc-amino acids, 6-chloro-1-hydroxy-benzotriazole (6-Cl–HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropylalcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). All reagents were used without further purification.
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