All of the chemicals and solvents were of HPLC or analytical grade. Analytical standards of 12 Has—IQ (2-amino-3-methyl-imidazo[4,5-f]quinoline), MeIQ (2-amino-3,4-dimethyl-imidazo[4,5-f]quinoline), MeIQx (2-amino-3,8-dimethyl-imidazo [4,5-f]quinoxaline), 4,8-DiMeIQx (2-amino-3,4,8-trimethyl-imidazo[4,5-f]quinoxaline), 7,8-DiMeIQx (2-amino-3,7,8-trimethyl-imidazo[4,5-f]quinoxaline), PhIP (2-amino-1- methyl-6-phenylimidazo[4,5-f]pyridine), Harman (1-methyl-9H-pyrido[3,4-b]indole), Norharman (9H-pyrido[3,4-b]indole), Trp-P-2 (3-amino-1-methyl-5H-pyrido[4,3-b] indole), Trp-P-1 (3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole), AαC (2-amino-9H- pyrido[2,3-b]indole), and MeAαC (2-amino-3-methyl-9H-pyrido[2,3-b]indole)—were supplied by Toronto Research Chemicals (Downsview, ON, Canada). The standards of free amino acids, creatine, and creatinine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Oasis MCX cartridges (60 mg, 3 mL) were supplied by Waters (Milford, MA, USA). The TRE was obtained followed our previous described [16 (link)] with the extraction conditions of extraction solvent of 60% ethanol, ultrasonic-assisted extraction power of 600 W for 40 min. The vacuum freeze dryer was employed to obtain the extract powders, which were stored at −20 °C until use.
Trp p 2
Manufactured by LGC
Sourced in Canada
Trp-P-2 is a chemical compound used in laboratory research. It functions as a mutagen, which can induce genetic mutations in biological samples. The core function of Trp-P-2 is to serve as a tool for studying mutagenic processes and their effects on living organisms. Further details on intended use or interpretation of its properties are not provided.
Automatically generated - may contain errors
Lab products found in correlation
Trp p 1, by LGC (6 mentions)
Meaαc, by LGC (6 mentions)
Glu p 2, by LGC (4 mentions)
Norharman, by LGC (3 mentions)
4 8 dimeiqx, by LGC (3 mentions)
7 8 dimeiqx, by LGC (3 mentions)
Glu p 1, by LGC (3 mentions)
Oasis mcx solid phase extraction cartridge, by Waters Corporation (2 mentions)
Meiqx, by LGC (2 mentions)
8 meiqx, by LGC (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Oasis mcx cartridge, by Waters Corporation (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Creatinine, by Merck Group (1 mentions)
Free amino acids, by Merck Group (1 mentions)
Creatine, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Harman, by LGC (1 mentions)
Milli q, by Merck Group (1 mentions)
6 protocols using trp p 2
1
Determination of Heterocyclic Amines in TRE
2
Quantification of Heterocyclic Amines in Food Samples
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White sesame seed was purchased from a local supermarket. The five adsorbents of activated carbons used in the present study were as follows: powdered type activated carbon made from peat (AP, F10), powdered type activated carbon made from wood (PW), powdered type activated carbon made from coconut shell (PC) and granular type activated carbon made from coconut shell (GC). F10, PW, PC and GC were purchased from Xin Sen Carbon Co., Ltd. (Nanping, China). AP was obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Acetonitrile (HPLC grade) was purchased from Thermo Fisher Scientific (Shanghai, China). Ammonium hydroxide and hydrochloric acid (HPLC grade) were obtained from Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Methyl alcohol, acetic acid and n-hexane were of HPLC grade, and other chemicals were of analytical reagent grade. Oasis MCX solid-phase extraction cartridge (150 mg, 6 mL) was purchased from Waters (Milford, CT, USA). Additionally, the water used was Wahaha purified water purchased from a local supermarket. The standards AαC, MeAαC, DMIP, Trp-P-1, Trp-P-2, Glu-P-2, MeIQ, MeIQx, IQ, PhIP, 4,8-DiMeIQx, 7,8-DiMeIQx were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Harman, Norharman and 4,7,8-TriMeIQx were purchased from Alta scientific (Tianjin, China).
3
Heterocyclic Aromatic Amines Analysis in Meat
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All chemicals and solvents were of HPLC or analytical grade. Acetonitrile, and Methanol were purchased from Merck (Germany). Formic acid, Hydrogen chloride (36.5–38%), Sodium chloride, and Magnesium sulfate was obtained from J.T.Baker (USA) was ACS grade. Ultrapure water (Milli-Q) was obtained from Millipore system (France). The heterocyclic aromatic amines (HCAs) were used as reference compounds: IQ, MeIQ, 8-MeIQx, PhIP, AαC, MeAαC, Harman, NorHarman, Trp-P-1, Trp-P-2, and Glu-P-1 were purchased from Toronto Research Chemicals (Canada). Standard stock solutions of 10 μg/g in Methanol were prepared and used for further dilution. 4,7,8-TriMeIQx was used as an internal standard (150 ng/g). The three types of sorbents used in QuEChERS are listed below: Primary and secondary amine (PSA) SPE bulk sorbent (Agilent Technologies, USA), SiliaBondR C18 (17%), 40–63 μm, 60 A (Silicycle® Inc, Canada), C18 Endcapped (C18EC), 57.9 μm, 59 A (Agilent Technologies, USA). Oasis® MCX Cartridge, 30 μm by Waters (USA). The analysis column was a Shim-pack GIST C18 (2.1 × 100 mm, 3 μm, 100 Å) from Shimadzu (Japan). Meat samples (pork floss, pork jerky, beef jerky, raw pork and raw beef) were purchased from local stores and stored in a freezer at −18 °C until used.
4
Quantification of Heterocyclic Amines
5
Quantifying Carcinogens in Pork Spareribs
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A total of 3 kg of raw pork spareribs (120 pieces) was procured from a local market located in New Taipei City, Taiwan. A total of 21 HA standards, including DMIP, IFP, PhIP, Phe-P-1, MeIQ, 4,8-DiMeIQx, 8-MeIQx, 7,8-DiMeIQx, IQx, IQ, IQ[4,5-b], Iso-IQ, MeAαC, AαC, Trp-P-2, Trp-P-1, Glu-P-2, Glu-P-1, Norharman, Harman, and internal standard 4,7,8-TriMeIQx were procured from Toronto Research Chemicals (Downsview, ON, Canada), while 24 PAH standards including DBaiP, DBahP, CcdP, DBahA, DBaeP, DBalP, BghiP, MCH, IP, CHR, BjF, BaA, BbF, BaP, BcF, Phe, Flu, Pyr, FL, Ant, AcP, NaP, AcPy, and internal standard Triphenylene were from Sigma-Aldrich Co. (St Louis, MO, USA). The full names of HA and PAH standards are shown in Tables S1 and S2 , respectively.
An Acquity UPLC BEH C18 column (100 × 2.1 mm ID, particle size 1.7 μm) used for HA separation was from Waters Co. (Milford, MA, USA), while a DB-5 MS capillary column (30 m × 0.25 mm ID, film thickness 0.25 μm) for PAH separation was from Agilent Technologies Co. (Palo Alto, CA, USA). The QuEChERS extraction and purification kits were from Yu-Ho Co (New Taipei City, Taiwan). The HPLC-grade solvents including methanol, acetonitrile, acetone, and hexane were from Merck (Darmstadt, Germany).
An Acquity UPLC BEH C18 column (100 × 2.1 mm ID, particle size 1.7 μm) used for HA separation was from Waters Co. (Milford, MA, USA), while a DB-5 MS capillary column (30 m × 0.25 mm ID, film thickness 0.25 μm) for PAH separation was from Agilent Technologies Co. (Palo Alto, CA, USA). The QuEChERS extraction and purification kits were from Yu-Ho Co (New Taipei City, Taiwan). The HPLC-grade solvents including methanol, acetonitrile, acetone, and hexane were from Merck (Darmstadt, Germany).
6
Quantification of Heterocyclic Aromatic Amines in Sesame Oils
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Commercial sesame seed oils, including pressed fragrant sesame seed oils (n = 7) and ground fragrant sesame seed oils (n = 7), were purchased from a local supermarket in China. Sesame seeds were purchased from a local supermarket in China. Acetonitrile (HPLC grade) was purchased from Thermo Fisher Scientific (Shanghai, China). Ammonium hydroxide and hydrochloric acid (HPLC grade) were obtained from Kemiou Chemical Reagent Co. Ltd. (Tianjin, China). Methyl alcohol, acetic acid and n-hexane were of HPLC grade, and other chemicals were of analytical reagent grade. Oasis MCX solid-phase extraction cartridge (150 mg, 6 mL) was purchased from Waters (Milford, PA, USA). The water used was Wahaha purified water purchased from a local supermarket. The standards AαC, MeAαC, DMIP, Trp-P-1, Trp-P-2, Glu-P-2, MeIQ, MeIQx, IQ, PhIP, 4,8-DiMeIQx and 7,8-DiMeIQx were purchased from Toronto Research Chemicals (Toronto, ON, Canada). Harman (1-methyl-9H-pyrido[3,4-b]indole), norharman (9H-pyrido[3,4-b]indole) and 4,7,8-TriMeIQx were purchased from Alta scientific (Tianjin, China).
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