Fei quanta 400 f

Manufactured by Thermo Fisher Scientific

Sourced in United States

The FEI Quanta 400 F is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of samples. It features a field emission electron source, which provides improved resolution and lower charging effects compared to traditional SEMs. The Quanta 400 F is capable of operating in high-vacuum, low-vacuum, and environmental modes, allowing for the examination of both conductive and non-conductive samples.

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Lab products found in correlation

Quanta 400f, by Thermo Fisher Scientific (1 mentions) Zetasizer nano z, by Malvern Panalytical (1 mentions) Panalytical xpert pro mpd diffractometer, by Malvern Panalytical (1 mentions) Eclipse ti u, by Nikon (1 mentions) Bh2 uma, by Olympus (1 mentions)

6 protocols using fei quanta 400 f

Interfacial Analysis of Pulp Chamber

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Interfacial and elemental analysis of the material was performed on the floor and wall of the pulp chamber using SEM and EDX (FEI Quanta 400F, Oregon, United States). At each section, three points were analyzed.
A thin conductive coating of gold was spurted to the polished sections prior to examination in the SEM. The SEM used was FEI Quanta 400F, equipped with an energy-dispersive spectrometer, wavelength-dispersive X-ray spectroscopy, and backscattering electron diffraction. The FEI Quanta 400F was operated under high vacuum mode with gaseous backscatter electron detector. Scanning electron micrographs of the material interfaces were captured at ×500. Quantitative elemental analysis of the products was done by EDX. Lines parallel to the interface at increasing incremental distances of 50 μm up to 400 μm were scanned and spectra collected were used to draw atomic ratio plots.

Suresh N., Chandrasekaran S., Ashritha M.C., Raoufe M.A., Vasudevan A, & Natanasabapathy V. (2023). Phosphoric acid treatment enhances adaptation of glass-ionomer cement to bioceramic sealer-conditioned dentin. Dental Research Journal, 20, 84.

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Nanosphere Size and Morphology Analysis

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Size distribution and polydispersity index (PDI) of the nanospheres were determined using the dynamic laser light scattering technique. Briefly, lyophilised nanospheres were dispersed in phosphate buffer saline (PBS, pH 7.4), transferred into a cuvette and subjected to laser light through the Zetasizer Nano ZS instrument (Malvern Instruments Ltd., Malvern, UK) for the measurement of particle diameter. Morphology of the nanospheres was studied via scanning electron microscopy (SEM) FEI Quanta 400 F (Hillsboro, OR, USA). Dried nanospheres were fixed onto metal stubs using double sided tape and sputter coated with a thin layer of gold under vacuum and observed under the microscope. All measurements were performed in triplicates at 25 °C, with a scattering angle of 90°.

Mahlumba P., Kumar P., du Toit L.C., Poka M.S., Ubanako P, & Choonara Y.E. (2021). Fabrication and Characterisation of a Photo-Responsive, Injectable Nanosystem for Sustained Delivery of Macromolecules. International Journal of Molecular Sciences, 22(7), 3359.

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Characterizing Coptidis Rhizoma Extract

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The Coptidis Rhizoma extract (150 mg) or pure berberine (30.9 mg) was added to 1 mL of water. The concentrations were in accordance with those used in the pharmacokinetic and acute oral toxicity studies. The suspensions were subjected to ultrasound for 1 h followed by centrifugation at 24,000 × g for 6 min. Aliquots (10 μL) of the supernatants were mounted on double-stick carbon tape fastened to specimen holders and dried. The dried spots were examined and photographed under an FEI Quanta 400 F scanning electron microscope (FEI Co., Hillsboro, OR, USA) operating at 10 kV.

Ma B.L., Yin C., Zhang B.K., Dai Y., Jia Y.Q., Yang Y., Li Q., Shi R., Wang T.M., Wu J.S., Li Y.Y., Lin G, & Ma Y.M. (2016). Naturally occurring proteinaceous nanoparticles in Coptidis Rhizoma extract act as concentration-dependent carriers that facilitate berberine absorption. Scientific Reports, 6, 20110.

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Comprehensive Material Characterization

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XRD-measurements were carried out with a Panalytical Xpert pro MPD diffractometer (Malvern Panalytical GmbH, Kassel, Germany) utilizing

C u K_{α 1} / K_{α 2}

-radiation at 40 kV and 40 mA. Detailed analysis of the diffractograms was performed via Rietveld refinement within the software MAUD (Luca Lutterotti, version 2.7, Trento, Italy). Scanning electron microscopy (SEM) measurements were done on a FEI Quanta 400f (Thermo Fischer Scientific, Gräfelfing, Germany), transmission electron microscopy (TEM) and selected area diffraction (SAED) as well as energy-dispersive X-ray (EDX)measurements on a JEOL 2200FS (JEOL, Freising, Germany).

Siebeneicher S., Waag F., Escobar Castillo M., Shvartsman V.V., Lupascu D.C, & Gökce B. (2020). Laser Fragmentation Synthesis of Colloidal Bismuth Ferrite Particles. Nanomaterials, 10(2), 359.

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Visualization and Characterization of Ablated Microchannels

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The geometry of ablated microchannels was observed by an optical microscope (Olympus BH2-UMA, Olympus Corporation, Tokyo, Japan) equipped with a digital camera (PROMICRA, Evropska, Czech Republic). Trypan Blue water (Yik Fung Scientific Co., Hong Kong, China) was injected into the microchannels for better visualization. Channel widths were measured across the microchannel by the software ImageJ. Hydrophobic treatment was carried out utilizing a water-repellent agent (47100, Aquapel, Pittsburgh, PA, USA). After injecting Aquapel into the microchannels, the specimen was baked at 50.0 °C on a hotplate until Aquapel dried out. To obtain a cross-sectional structure, the PbLT-fabricated chip was sliced utilizing the same CO₂ laser system. The cross-sectional structure of microchannels was examined by scanning electron microscopy (SEM) (FEI QUANTA 400F, Thermo Scientific, Waltham, MA, USA). Before SEM imaging, the specimen was sputter-coated with gold. Imaging and monitoring of bacteria cultivation were carried out with a fluorescence microscope (Eclipse Ti-U, Nikon, Tokyo, Japan).

Wei Y., Wang T., Wang Y., Zeng S., Ho Y.P, & Ho H.P. (2023). Rapid Prototyping of Multi-Functional and Biocompatible Parafilm®-Based Microfluidic Devices by Laser Ablation and Thermal Bonding. Micromachines, 14(3), 656.

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Scanning Electron Microscopy of Biomass

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The morphology of biomass was studied using a scanning electron microscope using FEI-Quanta 400 F (Thermo Fischer Scientific, US). The sample was placed on a carbon conductive tape stuck on pin mount specimen holder. The sample particles were pressed, and the air was blown to remove all the loosely bound particles. The samples were gold sputtered under vacuum for 120 s and kept in a desiccator before imaging.

Joy S.P., Ashok Kumar A., Gorthy S., Jaganathan J., Kunappareddy A., Gaddameedi A, & Krishnan C. (2021). Variations in structure and saccharification efficiency of biomass of different sorghum varieties subjected to aqueous ammonia and glycerol pretreatments. Industrial Crops & Products., 159.

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