Zen0040 cuvette

DLS experiments were performed using a Malvern Panalytical Zetasizer Pro at 25 °C, using a laser wavelength of 632.8 nm. The refractive indices of ASC and ASCb were determined to be 1.3327 using an Abbe Mark III refractometer (Reichart). The absorbance value of ASC and ASCb at 632.8 nm was 0.1089. ASC and ASCb samples were prepared at a concentration of ∼700 μM by weight in HPLC-grade water at pH 3.8. Sample concentration was accurately measured prior to each experimental run by absorbance at 280 nm for both ASC and ASCb. Samples were placed in a ZEN0040 cuvette (Malvern Panalytical) for small volumes and equilibrated for 2 min at 25 °C prior to each measurement. Measurements were taken at 30 min, 1 h, 3 h, 6 h, 10 h, 14 h, 16 h, 19 h, 23 h, 26 h, 31 h, 32 h, 44 h, 46 h, and 49 h after sample preparation. Photon counts of the scattered light versus time are fitted to correlation functions by the ZS Xplorer software, from which the size distribution and polydispersity values are obtained. Three measurements of the scattered light were taken at each time point, and the resulting data were plotted and analyzed with QtGrace (QTGroup, Helsinki, Finland).