The WT and mutant strains were grown under constant dark at 20 °C for 21 d and then transferred to 12 h dark:12 h light at 20 °C for 2 d. The hyphae and conidia were observed by light microscope and scanning electron microscope (SEM) (Hitachi SU8010, Tokyo, Japan). For the SEM, mycelia of about 0.5 cm2 with an agar disc were fixated with 2.5% glutaraldehyde (McLean Biochemical Technology, Shanghai, China) at 4 °C for 24 h. The glutaraldehyde was removed and the samples were eluted with deionized water three times. Subsequently, the deionized water was removed and samples were eluted with 70%, 85%, 95%, and 100% ethanol, respectively. Finally, the sample were glued on stubs, covered with a thin conductive layer and observed by SEM after critical-point drying and gold sputtering [31 (link)].
Glutaraldehyde
Manufactured by Maclin Biochemical Technology
Sourced in China
Glutaraldehyde is a chemical compound commonly used as a fixative and cross-linking agent in various laboratory applications. It is a colorless, oily liquid with a pungent odor. Glutaraldehyde is known for its ability to preserve and stabilize biological samples, proteins, and other macromolecules.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Maclin Biochemical Technology (10 mentions)
Ethanol, by Maclin Biochemical Technology (10 mentions)
Glycerol, by Maclin Biochemical Technology (9 mentions)
Tryptone, by Maclin Biochemical Technology (9 mentions)
Su8010, by Hitachi (4 mentions)
Sodium alginate, by Yuanye Bio-Technology (3 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by Maclin Biochemical Technology (2 mentions)
N n dimethylformamide (dmf), by Maclin Biochemical Technology (2 mentions)
Fetal bovine serum (fbs), by Wisent (2 mentions)
Glycerol, by Sinopharm (1 mentions)
Ammonium ferrous sulfate hexahydrate, by Sinopharm (1 mentions)
Glucose oxidase, by Maclin Biochemical Technology (1 mentions)
Hydrogen peroxide, by Sinopharm (1 mentions)
Glucose, by Sinopharm (1 mentions)
Ammonium hydroxide, by Sinopharm (1 mentions)
Sodium benzoate, by Sinopharm (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Live dead staining kit, by Thermo Fisher Scientific (1 mentions)
Fetal bovine plasma fbs, by Thermo Fisher Scientific (1 mentions)
26 protocols using glutaraldehyde
1
Scanning Electron Microscopy of Fungal Morphology
2
Peptide-Graphene Oxide Bioconjugate
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All chemicals used in this work are of analytical level. Peptide with the sequence of KIIIIKYWYAF was bought from the SynPeptide Biotechnology Co., Ltd. (Nanjing, China). Monolayered GO aqueous dispersions (GO-1, 10 mg/g) were provided by the Hangzhou Gaoxi Technology Co., Ltd. (Hangzhou, China). D-anhydrous glucose (AR), phosphoric acid, ethanol, glycerol, glutaraldehyde, NaCl, tryptone, and agar powder were purchased from the Shanghai Maclean Biochemical Technology Co., Ltd. (Shanghai, China).
3
Alginate-Xylanase Hybrid Hydrogel
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Sodium alginate (biochemical grade, transferred to fix cells, and enzymes, etc.) and anhydrous calcium chloride were purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). glutaraldehyde from Shanghai Maclean Biochemical Technology Co. (Shanghai, China), and beechwood xylan and 3,5-dinitrosalicylic acid from hanghai Aladdin Biochemical Technology Co. (Shanghai, China). Maleic anhydride-modified xylanase (MA-XY) and wheat bran were made in the laboratory. All other reagents and chemicals used in this study were of analytical grade.
4
Fabrication of Vascular Graft Scaffolds
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Polyester plain fabrics were bought in local chemical fiber industrial (Fa Xinrui Textile Co., Qingdao, China). Medical grade polyurethane (PU-Pellethane® 2363-80AE TPU) were purchased from Dow Chemical (Guangzhou, China). Glycerol was obtained from Sinopharm Chemical Reagent (Shanghai, China). Sodium citrate anticoagulant whole blood was acquired from Beijing Bersee Science and Technology (Beijing, China). Medical grade gelatin (type A from bovine skin, ~250 bloom), glutaraldehyde (25% commercial aqueous reagent), and N,N-dimethylformamide (DMF) were supplied by Shanghai Macklin Biochemical (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) were obtained from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences, Shanghai, China). Cell culture medium and reagents were provided by Aladdin Life Technologies (Shanghai, China).
5
Chitosan-based Biosensor Development
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Chitosan (food grade, DAC degree: 86%, viscosity: 296 mPa s) was supplied by Zhejiang Golden-Shell Pharmaceutical Co., Ltd. Glutaraldehyde (25%), trichloroethylene and glucose oxidase (from Aspergillus niger, 100 U mg−1) were supplied by Shanghai Macklin Biochemical Co., Ltd. Ammonium ferrous sulfate dodecahydrate (NH4Fe(SO4)2·12H2O), ammonium ferrous sulfate hexahydrate ((NH4)2Fe(SO4)2·6H2O), glucose, 4-hydroxybenzoic acid, hydrogen peroxide (30% w/w), ammonium hydroxide (25%), sodium benzoate and other chemicals were purchased from Sinopharm Chemical Reagent Co., Ltd, China. All chemicals used were of analytical grade or above.
6
Antibacterial Gelatin-Glycerin Hydrogel Dressing
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Gelatin (type A, from porcine skin, 300 Bloom) and glycerin (≥99.0%) were purchased from Sigma-Aldrich. (NH4)2SO4 (≥99.5%) was purchased from Titan (Shanghai), Inc. Glutaraldehyde (50% in H2O) was supplied by Macklin. Ca(NO3)2·4H2O (≥99.0%) was provided by Sinopharm Chemical Co. Ltd. Deionized water was used in the experiments. 3 M Tegaderm™ Hydrocolloid Thin Dressing (90022 T) was purchased from Minnesota Mining and Manufacturing Co. (Taiwan). Staphylococcus aureus was donated by the School of Pharmacy of Fudan University. Dulbecco's Modified Eagle's Medium (DMEM), fetal bovine plasma (FBS), phosphate buffered saline (PBS), and penicillin-streptomycin were all purchased from Gibco (Thermo Fisher Scientific, Inc.). The CCK-8 reagent was supplied by Beyotime (Shanghai, China). A Live/dead staining kit was obtained from Invitrogen Corporation (CA, USA). The L929 fibroblast cells were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). All the reagents were used as received.
7
Enzyme-Functionalized Alginate Beads
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BC membrane was obtained from Hainan Yeguo Foods Co., Ltd (Hainan, China). Sodium alginate, TEA, glutaraldehyde (aqueous solution, 50 wt %), sodium hydroxide, and calcium chloride were purchased from Macklin Biochemical Co., Ltd (Shanghai, China). ALPs extracted from the calf intestine were obtained from AppliChem GmbH (Darmstadt, Germany). CaGP, 4-nitrophenyl phosphate disodium salt hexahydrate, and p-nitrophenol were bought from Alfa Aesar Chemicals Co., Ltd (Shanghai, China). Hydrochloric acid and dimethyl sulfoxide were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
8
Ultrastructural Analysis of Cells
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Cells (1 × 106) were prefixed with glutaraldehyde for 2 h (Macklin, Wuhan, China) and embedded in osmium acid solution. Subsequent processing was completed as previously described [18 (link)]. The ultrastructure of the samples was observed at the Analysis and Testing Center of Jinan University.
9
Electron Microscopic Sample Preparation
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Samples were fixed with 2.5 % glutaraldehyde (Macklin, Shanghai, China) at 4 °C for 4 h and subsequently dehydrated and dried. After slicing into ultrathin sections, the cells were stained with uranyl acetate and lead citrate. Samples were then photographed using a Tecnai-10 microscope (Philips, Amsterdam, Netherlands).
10
Chitosan-based Biomaterial Synthesis
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Chitosan (deacetylation degree ≥95%, viscosity from 100 to 200 mpa.s.), sodium-hydroxide, chlorosulfonic acid, lithium bromide, glycidyl methacrylate, acetic acid and anhydrous ethanol were obtained from Aladdin (Wuhan, China). N-N dimethylformamide, anhydrous sodium carbonate and glutaraldehyde were purchased from Macklin (Shanghai, China). Dulbecco’s modified Eagle medium (DMEM), penicillin–streptomycin, fetal bovine serum (FBS) and phosphate buffer saline (PBS) were supplied by Gibco (Grand Island, NY, USA). A live/dead cell double-staining kit was obtained from Proteintech (Rosemont, IL, USA), and a cell counting kit-8 was purchased from KeyGen Biotech (Nanjing, China). Enzyme-linked immunosorbent assay kits were obtained from 4A Biotech (Beijing, China). Streptozotocin was obtained from Sigma Aldrich (Burlington, MA, USA). Citric acid was purchased from Solarbio (Beijing, China). Sodium citrate was supplied by YuanYe Biotech (Shanghai, China). A hematoxylin and eosin dying kit was purchased from Beyotime (Shanghai, China), and Masson’s Trichrome dying kit was purchased from Solabio (Beijing, China), dying according to the procedure in the instructions.
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