Western blot was carried out according as described [41 (link), 42 (link)] with rabbit polyclonal PLOD2, E-cadherin and β-catenin antibodies (1:1000; Proteintech, USA), PI3K, p-PI3K (Tyr458), AKT, p-AKT (Ser473), GSK-3β, p-GSK-3β, N-Cadherin, Slug and Vimentin antibodies (1:1000; Cell Signaling Technology, Danvers, MA, USA), as well as Snail antibody (1:1000; Abcam, USA). Mouse monoclonal HIF-1α antibody (1:50; Novus Biologicals, USA) was used for normalization. An HRP-conjugated anti-rabbit or anti-mouse IgG antibody was used as the secondary antibody (1:2000; CoWin Bioscience, Beijing, China). Signals were detected using enhanced chemiluminescence reagents (Pierce, Rockford, IL, USA).
Snail antibody
Manufactured by Abcam
Sourced in United States
The Snail antibody is a primary antibody used for the detection and analysis of the Snail protein, a transcriptional regulator involved in the epithelial-mesenchymal transition process. This antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to study the expression and localization of the Snail protein in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
P akt ser473, by Cell Signaling Technology (1 mentions)
E cadherin, by Proteintech (1 mentions)
P gsk3β, by Cell Signaling Technology (1 mentions)
Plod2, by Proteintech (1 mentions)
Hif 1α antibody, by Novus Biologicals (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
β catenin, by Proteintech (1 mentions)
Sybr green, by Qiagen (1 mentions)
Control sirna, by Santa Cruz Biotechnology (1 mentions)
Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions)
Opti mem reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Mouse β actin antibody, by Santa Cruz Biotechnology (1 mentions)
Thermoscript rt pcr system, by Thermo Fisher Scientific (1 mentions)
E cadherin antibody, by Santa Cruz Biotechnology (1 mentions)
Vegf antibody, by Santa Cruz Biotechnology (1 mentions)
Vimentin antibody, by Santa Cruz Biotechnology (1 mentions)
Twist antibody, by Santa Cruz Biotechnology (1 mentions)
Groα elisa kit, by R&D Systems (1 mentions)
4 protocols using snail antibody
1
Western Blot Analysis of EMT Pathway
2
Regulation of EMT by GROα in Tumor Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Materials were purchased from various manufacturers: recombinant GROα (R&D Systems), GROα siRNA (Santa Cruz Biotechnology), control siRNA (Santa Cruz Biotechnology), Lipofectamine ltx (Invitrogen), Opti-MEM Reduced Serum medium (Invitrogen), GROα ELISA kit (R&D Systems), GROα primers (IDT), ThermoScript™ RT-PCR systems (Invitrogen), SYBR Green (Qiagen), EMT marker primers (IDT), PD98059 (Cell Signaling), E-cadherin antibody (Santa Cruz Biotechnology), N-cadherin antibody (Santa Cruz Biotechnology), Snail antibody (Abcam), Slug antibody (Abcam), Twist antibody (Santa Cruz Biotechnology), Vimentin antibody (Santa Cruz Biotechnology), VEGF antibody (Santa Cruz Biotechnology), phospho-MAPK antibody (Cell Signaling), MAPK antibody (Cell Signaling), β-actin mouse antibody (Santa Cruz Biotechnology).
3
Evaluating EMT and Apoptosis Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cells of each group were collected and lysed, while the total protein was extracted. The protein concentration was examined by BCA, and we adjusted the protein concentration of each group. We loaded samples 30 μg/well, and electrophoresis was performed with SDS-PAGE gel. The protein was transferred to the PVDF membrane, sealed with skim milk at 25°C, incubated with primary antibody at 4°C overnight, incubated with HRP-linked secondary antibody, developed and photographed with an ECL kit. Primary antibodies included Snail antibody (ab216347), Twist antibody (ab50887), E-cadherin antibody (ab231303), N-cadherin antibody (ab76011), GSK3β antibody (ab32391), β-catenin antibody (ab32572), Bax antibody (ab32503), Bcl-2 antibody (ab182858), and GAPDH antibody (ab8245), all derived from Abcam.
4
Western Blot Analysis of Cell Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The monoclonal human CAP1 antibody was previously described50 (link). Antibodies against GAPDH and GSK3 beta antibodies were from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). E-cadherin antibody was from BD Biosciences (Franklin Lakes, NJ). Snail antibody was from Abcam (Cambridge, MA), and antibodies against ERK1/2, phosphor-ERK1/2 (Thr202/Tyr204), phosphor-cofilin, phospho-GSK3α/β (Ser21/Ser9), and FAK and phosphor-Y397 FAK were from Cell Signaling Technology Inc. (Danvers, MA). Cofilin antibody was from the Cytoskeleton Inc. (Denver, CO). Cell lysates were prepared for Western blotting similarly as previously described6 (link)51 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!