Rabbit anti mob1

Manufactured by Cell Signaling Technology

Rabbit anti-MOB1 is a primary antibody that recognizes the MOB1 protein. MOB1 is a key regulator of the Hippo signaling pathway, which plays a crucial role in cell proliferation, differentiation, and apoptosis. This antibody can be used to detect and study the expression and localization of MOB1 in various biological samples.

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Lab products found in correlation

Mouse anti α actinin, by Merck Group (1 mentions) Anti β actin hrp conjugate, by Cell Signaling Technology (1 mentions) Anti ki67, by Abcam (1 mentions) Phenylephrine, by Merck Group (1 mentions) Ab36999, by Abcam (1 mentions) Rabbit anti mbp, by New England Biolabs (1 mentions) Rabbit anti flag, by Merck Group (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Mouse anti gfp, by BD (1 mentions) Anti α tubulin dm1a, by Merck Group (1 mentions) Rabbit anti γ tubulin, by Abcam (1 mentions) Rabbit anti rfp, by Thermo Fisher Scientific (1 mentions) Mouse anti eb1, by BD (1 mentions) Rabbit anti histone h3, by Cell Signaling Technology (1 mentions) Rabbit anti erk1 2, by Cell Signaling Technology (1 mentions) Rabbit anti yap taz, by Cell Signaling Technology (1 mentions) Rabbit anti p erk1 2, by Cell Signaling Technology (1 mentions) Rabbit anti mst1, by Cell Signaling Technology (1 mentions) Rabbit anti brd4, by Fortis Life Sciences (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions)

3 protocols using rabbit anti mob1

Molecular Mechanisms of YAP Regulation

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XMU‐MP‐1 (Fan et al., 2016) was obtained from MedChem Express. Phenylephrine was obtained from Sigma (P6126). Antibodies used in this study include mouse anti‐α‐actinin (Sigma, cat no A7811), rabbit anti‐phospho‐MOB1 (Cell Signalling Technology, #8699S), rabbit anti‐MOB1 (Cell Signalling Technology, #13730S), rabbit anti‐active‐YAP (Cell Signalling Technology, #29495S), anti‐β‐actin HRP conjugate (Cell Signalling Technology, #122625), and anti‐Ki67 (Abcam, ab15580). Secondary antibodies used are Alexa Fluor® 647 anti‐mouse IgG (Jackson ImmunoReasearch Laboratories, Inc., 115‐605‐003), Alexa Fluor® 488 anti‐rabbit IgG (Jackson ImmunoReasearch Laboratories, Inc., 111‐545‐144), and anti‐rabbit IgG (Cell Signalling Technology, #7074S).

Triastuti E., Nugroho A.B., Zi M., Prehar S., Kohar Y.S., Bui T.A., Stafford N., Cartwright E.J., Abraham S, & Oceandy D. (2019). Pharmacological inhibition of Hippo pathway, with the novel kinase inhibitor XMU‐MP‐1, protects the heart against adverse effects during pressure overload. British Journal of Pharmacology, 176(20), 3956-3971.

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Monoclonal and Polyclonal Antibody Characterization

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Anti-NDR1 monoclonal antibody (M04) was purchased from Abnova. Anti-pT444-NDR1 rabbit polyclonal antibody was produced and purified as described previously40 (link). Anti-Ezrin monoclonal antibody (4A5) was described previously41 (link). Other antibodies were used as following: mouse anti-GFP (BD Biosciences); mouse anti-Flag (M2, Sigma); anti-α-tubulin (DM1A, Sigma); rabbit anti-Flag (Sigma, F-7425); rabbit anti-γ-tubulin (Abcam); mouse anti-actin (1A4, Sigma, A-2547); mouse anti-EB1 (BD Biosciences, 610534); mouse anti-Cyclin B (BD Biosciences, 554177); rabbit anti-RFP (Invitrogen, R10367); rabbit anti-Mob1 (Cell Signaling, 3863); rabbit anti-Mob2 (HCCA2) (Abcam, ab103109); rabbit anti-MBP (New England BioLabs, E8032); rabbit anti-pT210-PLK1 (Epitomics, 3646-1); rabbit anti-NuMA (Abcam, ab36999) and ACA (a gift from Dr. Don Cleveland, University of California at San Diego, La Jolla, CA).

Yan M., Chu L., Qin B., Wang Z., Liu X., Jin C., Zhang G., Gomez M., Hergovich A., Chen Z., He P., Gao X, & Yao X. (2015). Regulation of NDR1 activity by PLK1 ensures proper spindle orientation in mitosis. Scientific Reports, 5, 10449.

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Protein Isolation and Western Blot Analysis

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Protein isolation and subsequent Western blot analysis was performed as described previously (66 (link)). The following antibodies were used: rabbit anti-YAP/TAZ (Cell Signaling Technology, catalog 8418, RRID:AB_10950494), rabbit anti–Histone H3 (Cell Signaling Technology, catalog 9717, RRID:AB_331222), mouse anti-GAPDH (Santa Cruz Biotechnology Inc., catalog sc-47724, RRID:AB_627678), rabbit anti-BRD4 (Bethyl, catalog A700-004, RRID:AB_2631885), rabbit anti-ERK1/2 (Cell Signaling Technology, catalog 4695, RRID:AB_390779), rabbit anti–p-ERK1/2 (Cell Signaling Technology, catalog 4370, RRID:AB_2315112), rabbit anti-LATS1 (Proteintech, catalog 17049-1-AP, RRID:AB_2281011), rabbit anti-LATS2 (Proteintech, catalog 20276-1-AP, RRID:AB_10697657), rabbit anti-MST1 (Cell Signaling Technology, catalog 3682, RRID:AB_2144632), rabbit anti-MST2 (Cell Signaling Technology, catalog 3952, RRID:AB_2196471), rabbit anti-NF2 (Thermo Fisher Scientific, catalog PA5-82060, RRID:AB_2789221), rabbit anti-SAV1 (Cell Signaling Technology, catalog 13301, RRID:AB_2798176), and rabbit anti-MOB1 (Cell Signaling Technology, catalog 13730, RRID:AB_2783010).

Chen Z., Li S., Mo J., Hawley E., Wang Y., He Y., Brosseau J.P., Shipman T., Clapp D.W., Carroll T.J, & Le L.Q. (2020). Schwannoma development is mediated by Hippo pathway dysregulation and modified by RAS/MAPK signaling. JCI Insight, 5(20), e141514.

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