Dechorionated embryos were fixed and stained as per Roddie et al.31 . Antibodies were diluted in PATx (0.1% Triton-X100 (Sigma-Aldrich), 1% BSA (Sigma-Aldrich) in PBS (Oxoid, Thermo Fisher, MA, USA)). Rabbit anti-GFP (ab290 1:1000; Abcam, Cambridge, UK) or mouse anti-GFP (ab1218 1:200; Abcam) were used to detect GFP-labelled macrophages. Rabbit anti-cDCP-1 (9578S 1:1000; Cell Signaling Technologies), mouse anti-Repo (concentrate of clone 8D12 used at 1:1000; Developmental Studies Hybridoma Bank, University of Iowa, USA) or mouse anti-Futch (supernatant of clone 22C10 used at 1:200; Developmental Studies Hybridoma Bank) were also used as primary antibodies. Goat anti-mouse or goat anti-rabbit secondary antibodies conjugated to AlexaFluor568, AlexaFluor488 (A11036 and A11034; Invitrogen, Thermo Fisher) or FITC (115-095-146; Jackson Immunoresearch, Cambridge, UK) were used to detect primary antibodies; these were diluted from stock solutions made according to the recommendations of the supplier (1:400 in PATx). Stained embryos were stored in DABCO mountant (Sigma-Aldrich) and mounted on slides for imaging.
Mouse anti futch
Manufactured by Developmental Studies Hybridoma Bank
Sourced in United States
Mouse anti-Futch is a laboratory reagent used for the detection and identification of the Futch protein in biological samples. It is a monoclonal antibody produced by hybridoma technology. This product is intended for research use only and its specific applications should be determined by the end-user.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (2 mentions)
Alexa fluor 568, by Thermo Fisher Scientific (2 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Fluorescein isothiocyanate (fitc), by Jackson ImmunoResearch (2 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (2 mentions)
Ab290, by Abcam (2 mentions)
Ab1218, by Abcam (2 mentions)
Dabco mountant, by Merck Group (2 mentions)
Mouse anti repo, by Developmental Studies Hybridoma Bank (2 mentions)
A11034, by Thermo Fisher Scientific (1 mentions)
A11036, by Thermo Fisher Scientific (1 mentions)
2 protocols using mouse anti futch
1
Immunostaining of Drosophila Embryos
2
Immunohistochemical Staining of Drosophila Embryos
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dechorionated embryos were fixed and stained as per Roddie et al., 2019 [29] .
Antibodies were diluted in PATx (0.1% Triton-X100 (Sigma-Aldrich), 1% BSA (Sigma-Aldrich) in PBS (Oxoid, Thermo Fisher, MA, USA)). Rabbit anti-GFP (ab290 1:1000; Abcam, Cambridge, UK) or mouse anti-GFP (ab1218 1:200; Abcam) were used to detect GFP-labelled macrophages. Rabbit anti-cDCP-1 (9578S 1:1000; Cell Signaling Technologies), mouse anti-Repo (concentrate of clone 8D12 used at 1:1000; Developmental Studies Hybridoma Bank, University of Iowa, USA) or mouse anti-Futch (supernatant of clone 22C10 used at 1:200; Developmental Studies Hybridoma Bank) were also used as a primary antibodies. Goat anti-mouse or goat anti-rabbit secondary antibodies conjugated to AlexaFluor568, AlexaFluor488 (Invitrogen, Thermo Fisher) or FITC (Jackson Immunoresearch, Cambridge, UK) were used to detect primary antibodies; these were diluted from stock solutions made according to the recommendations of the supplier (1:400 in PATx). Stained embryos were stored in DABCO mountant (Sigma-Aldrich) and mounted on slides for imaging.
Antibodies were diluted in PATx (0.1% Triton-X100 (Sigma-Aldrich), 1% BSA (Sigma-Aldrich) in PBS (Oxoid, Thermo Fisher, MA, USA)). Rabbit anti-GFP (ab290 1:1000; Abcam, Cambridge, UK) or mouse anti-GFP (ab1218 1:200; Abcam) were used to detect GFP-labelled macrophages. Rabbit anti-cDCP-1 (9578S 1:1000; Cell Signaling Technologies), mouse anti-Repo (concentrate of clone 8D12 used at 1:1000; Developmental Studies Hybridoma Bank, University of Iowa, USA) or mouse anti-Futch (supernatant of clone 22C10 used at 1:200; Developmental Studies Hybridoma Bank) were also used as a primary antibodies. Goat anti-mouse or goat anti-rabbit secondary antibodies conjugated to AlexaFluor568, AlexaFluor488 (Invitrogen, Thermo Fisher) or FITC (Jackson Immunoresearch, Cambridge, UK) were used to detect primary antibodies; these were diluted from stock solutions made according to the recommendations of the supplier (1:400 in PATx). Stained embryos were stored in DABCO mountant (Sigma-Aldrich) and mounted on slides for imaging.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!