High Sensitivity C Reactive Protein (hsCRP) was measured in serum or plasma using the Cardiac C-reactive Protein (Latex) Sensitive immunoturbidimetric assay on the automated Roche/Hitachi cobas c 311 module. The lower limit of detection, as determined by the assay manufacturer, is 0.5 mg/L. Additional details of the laboratory procedures can be found here (NAHDAP, 2018b ).
Interleukin 6 (IL-6) IL-6 assays were performed following GenWay Biotech’s Standard Operating Procedure ANA015 (High Sensitivity Human IL-6 in Serum ELISA). IL-6 was measured in serum using the Quantikine Human IL-6 ELISA KIT (R&D Systems Cat# HS600B and HS600C) and Immunoassay Control Group 10 (R&D Systems Cat#QC41). Optical density was read using the Emax precision microplate reader (Molecular Devices) set to 490 nm. The assay range is 0.255 to 9.755 pg/mL.
Fibrinogen was measured using the Clauss fibrinogen assay, a quantitative, clot-based, functional assay. The assay measures the ability of fibrinogen to form fibrin clots after being exposed to a high concentration of purified thrombin. The assay was run on the ACL Top 300, which adds a predetermined number of units of bovine thrombin to citrated human plasma and measures the clotting kinetics turbidometrically. The assay range is 150 to 1000 mg/dL.
Interleukin 6 (IL-6) IL-6 assays were performed following GenWay Biotech’s Standard Operating Procedure ANA015 (High Sensitivity Human IL-6 in Serum ELISA). IL-6 was measured in serum using the Quantikine Human IL-6 ELISA KIT (R&D Systems Cat# HS600B and HS600C) and Immunoassay Control Group 10 (R&D Systems Cat#QC41). Optical density was read using the Emax precision microplate reader (Molecular Devices) set to 490 nm. The assay range is 0.255 to 9.755 pg/mL.
Fibrinogen was measured using the Clauss fibrinogen assay, a quantitative, clot-based, functional assay. The assay measures the ability of fibrinogen to form fibrin clots after being exposed to a high concentration of purified thrombin. The assay was run on the ACL Top 300, which adds a predetermined number of units of bovine thrombin to citrated human plasma and measures the clotting kinetics turbidometrically. The assay range is 150 to 1000 mg/dL.