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Nanodrop 2000c 2000 uv vis spectrophotometer

Manufactured by Thermo Fisher Scientific
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The NanoDrop 2000c/2000 UV-Vis spectrophotometer is a compact, microvolume instrument designed for the analysis of nucleic acids and proteins. It utilizes a patented sample retention system to accurately measure samples as small as 0.5 microliters. The NanoDrop 2000c/2000 provides high-quality absorbance measurements in the UV-Vis spectral range.

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Lab products found in correlation

Pmdtm19 t vector cloning kit, by Takara Bio (1 mentions) Tianprep mini plasmid kit, by Tiangen Biotech (1 mentions) Qiaamp dna blood mini kit, by Qiagen (1 mentions)

Close spelling variants of this product

NanoDrop 2000/2000c Spectrophotometer 2000c UV-Vis spectrophotometer NanoDrop 2000 UV-Vis NanoDrop UV-Vis spectrophotometer NanoDrop 2000/2000c NanoDrop 2000c spectrophotometer UV-Vis Nanodrop NanoDrop 2000 UV NanoDrop 2000 spectrophotometer NanoDrop UV spectrophotometer

8 protocols using nanodrop 2000c 2000 uv vis spectrophotometer

1

Cloning of CYP2C19 Variant Plasmids

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Plasmids 19-2GG, 19-2AA, 19-3GG and 19-3AA, which contain the CYP2C19*2 G681G, CYP2C19*2 A681A, CYP2C19*3 G636G, and CYP2C19*3 A636A genes, respectively, were constructed using the pMDTM19-T Vector Cloning Kit (Takara Bio Inc. Dalian, China) and were extracted from transformed Escherichia coli DH5α cells using a TIANprep Mini Plasmid Kit (Tiangen Biotech Co., Ltd. Beijing, China). The plasmids were identified via sequencing by Beijing Genomic Institute (BGI, Beijing, China). The concentrations were determined using a NanoDrop 2000c/2000 UV-Vis spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA).
Zhang C., Yao Y., Zhu J.L., Zhang S.N., Zhang S.S., Wei H., Hui W.L, & Cui Y.L. (2016). Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms. Scientific Reports, 6, 26533.
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2

Whole Blood DNA Isolation from Chinese Volunteers

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Peripheral blood samples were collected from 100 unrelated Chinese volunteers using EDTA-coated tubes at the Shaanxi Provincial People’s Hospital (Xi’an, China) with informed consent. The study was approved by the ethics committee of the National Engineering Research Center for Miniaturized Detection Systems, Xi’an, China. All methods were performed in accordance with these approved guidelines. The genomic DNA from the volunteer was isolated from 200 μL of blood using a Whole Blood Genomic DNA Isolation Kit (Xi’an GoldMag Nanobiotech Co., Ltd., Xi’an, Shaanxi, China), according to the manufacturer’s instructions. The final DNA quality and concentrations were measured using a NanoDrop 2000c/2000 UV-Vis spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA), according to the manufacturer’s instructions.
Zhang C., Yao Y., Zhu J.L., Zhang S.N., Zhang S.S., Wei H., Hui W.L, & Cui Y.L. (2016). Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms. Scientific Reports, 6, 26533.
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3

Gemcitabine pH-Dependent Release Kinetics

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Three vials of pH 8.0–9.0 gemcitabine + CMG encapsulation solution (2 mL) were centrifuged 30 minutes at 12,500 RPM. The supernatant was aspirated and the CMG-Gem nanoparticles were re-suspended in 2 mL of pH 7.4, 6.5, or 6.0 PBS. All samples remained under magnetic stirring at room temperature for 96 h. These nanoparticles were characterized by NanoDrop 2000c/2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Gemcitabine release was calculated by UV-Vis absorbance at 275 nm with 3 separate measurements of two drops of from each sample, for a total of 6 measurements per pH condition per time point. An average of the values was plotted as the UV-VIS absorbance for that time point. Prior to each supernatant sampling, each sample was spun for 10 minutes at 10,000 RPM to precipitate the nanoparticles. Percentage gemcitabine release was calculated by dividing the average value for each pH sample measurement at a time point by the maximum UV-Vis value measured for that pH value over a 96 hour period.
Zeiderman M.R., Morgan D.E., Christein J.D., Grizzle W.E., McMasters K.M, & McNally L.R. (2016). Acidic pH-targeted chitosan capped mesoporous silica coated gold nanorods facilitate detection of pancreatic tumors via multispectral optoacoustic tomography. ACS biomaterials science & engineering, 2(7), 1108-1120.
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4

Gemcitabine Release Kinetics from MSNs

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Gemcitabine loaded MSNs (3 mL) were titrated to a pH of 6.5, 6.8, or 7.4 by addition of NaOH or HCI and placed inside dialysis tubing. The samples were then placed in a solution of PBS (15mL) at a matching pH. The samples were stirred continuously throughout the experiment. Readings of the PBS solutions were analyzed with the NanoDrop 2000c/2000 UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). A solution for PBS was used as blank for the spectrophotometer. It was possible to determine the amount of Gemcitabine released from the MSNs by analyzing the dialysis fluid. Samples were read consecutively every hour for 10 hours and absorbance values were plotted to determine the drug release kinetics of Gemcitabine from the MSNs.
Gurka M.K., Pender D., Chuong P., Fouts B., Sobelov A., McNally M., Mezera M., Woo S, & McNally L.R. (2016). Identification of pancreatic tumors in vivo with ligand-targeted, pH responsive mesoporous silica nanoparticles by multispectral optoacoustic tomography. Journal of controlled release : official journal of the Controlled Release Society, 231, 60-67.
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5

Quantitative Real-time PCR Analysis of Apoptotic Markers

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Quantitative Real-time PCR was operated on Rotor-Gene Q® PCR system as a reader (Cat.#: 204774; QiagenTM, Milan, Italy) via GenElute® RNA extraction/SIGMA PCR kit (Cat.#: REI10; QiagenTM, Milan, Italy)86. Cells were treated with IC50 of 8b for 48 h and total RNA was extracted from the non-treated and treated cells. RNA purity was assessed via Nanodrop® 2000/2000c UV-Vis spectrophotometer (Thermo-ScientificTM, Bilbao, Spain). Synthesis of cDNA was proceeded using QuantiNova® Reverse Transcription Kit (Cat.#: RTN30; QiagenTM, Milan, Italy) and the subsequent PCR tests were conducted via single tubes. Specific forward/reverse primer pairs were selected for investigated (Casp-3, -8, -9, BAX, and Bcl2). Obtained results were expressed within Cycle threshold (Ct) values, while relative quantitation of each measured gene was assessed based on ΔΔCt calculations as represented in Table 5 [79 (link)].
Nagy M.I., Darwish K.M., Kishk S.M., Tantawy M.A., Nasr A.M., Qushawy M., Swidan S.A., Mostafa S.M, & Salama I. (2021). Design, Synthesis, Anticancer Activity, and Solid Lipid Nanoparticle Formulation of Indole- and Benzimidazole-Based Compounds as Pro-Apoptotic Agents Targeting Bcl-2 Protein. Pharmaceuticals, 14(2), 113.
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6

DNA Extraction from Whole Blood

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Venous blood (3 mL) was collected in vials containing EDTA for DNA extraction and stored at −80 °C until extraction. DNA was extracted from whole blood using a Blood Genomic DNA Extraction Mini Kit (ALPHAGEN Biotech Ltd., Taiwan) according to the manufacturer’s instructions. Briefly, 200 μL of whole blood was incubated at 60°C with proteinase K and 200 μL BG Buffer for 15 min by vortexing the sample every 5 min. Ethanol (200 μL, 99.5%) was added, transferred to a BG mini-column, centrifuged, and the mini-column placed in a new collection tube. The column was washed once with 200 μL GW Buffer and then with 750 μL Wash Buffer. After drying, the DNA was eluted in 100 μL of preheated Elution Buffer by centrifugation of the BG mini-column at full speed. DNA was quantified using a NanoDrop 2000/2000c UV/VIS Spectrophotometer (ThermoScientific™). To assess the purity of DNA, the absorbance at 260 nm (A260) was divided by the absorbance at 280 nm (A280). An A260/A280 ratio of 1.7–2.0 was considered good-quality DNA devoid of proteins. Extracted DNA samples were stored at −20 °C until genotyping.
Degaga A., Sirgu S., Huri H.Z., Sim M.S., Kebede T., Tegene B., Loganadan N.K., Engidawork E, & Shibeshi W. (2023). Association of Met420del Variant of Metformin Transporter Gene SLC22A1 with Metformin Treatment Response in Ethiopian Patients with Type 2 Diabetes. Diabetes, Metabolic Syndrome and Obesity, 16, 2523-2535.
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7

Buccal Swab DNA Extraction and Genotyping

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Genomic DNA was isolated from buccal swabs using a standard salt lysis method [32 (link)]. Extracted DNA samples were stored at −20 °C. DNA was quantified using a NanoDrop™2000/2000c UV/VIS Spectrophotometer (ThermoScientific™). SNPs were genotyped using the MassARRAY®System IPLEX extension reaction (Agena Bioscience™). Genotypes of the selected SNP variants were determined for all the study participants.
Masilela C., Pearce B., Ongole J.J., Adeniyi O.V, & Benjeddou M. (2021). Single Nucleotide Polymorphisms Associated with Metformin and Sulphonylureas’ Glycaemic Response among South African Adults with Type 2 Diabetes Mellitus. Journal of Personalized Medicine, 11(2), 104.
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8

DNA Extraction from Blood and Tissue

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Genomic DNA was isolated from peripheral blood samples of all patients and from ventricular tissues of abortions using QIAamp DNA Blood Mini Kit (Qiagen, Hilden, Germany). NanoDrop 2000/2000c UV-vis spectrophotometer (ThermoFisher Scientific, Waltham, Massachusetts, US) was used to determine the quantity and quality of DNA.
Nagy O., Szakszon K., Biró B.O., Mogyorósy G., Nagy D., Nagy B., Balogh I, & Ujfalusi A. (2019). Copy number variants detection by microarray and multiplex ligation-dependent probe amplification in congenital heart diseases. Journal of biotechnology, 299.
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