Glass microfiber filters gf a

Acetonitrile and methanol were reagent grade or better and were purchased from Carlo Erba Reagents (Milan, Italy). Ultrapure water was produced by a Milli-Q^® Direct system (Merck KGaA, Darmstadt, Germany). T-2 and HT-2, sodium chloride (NaCl), sodium azide (NaN₃), phosphate buffer saline (PBS) and ovalbumin (OVA) were purchased from Sigma-Aldrich (Milan, Italy). Ten monoclonal antibodies (MAbs) specific for T-2G (clones 1–2, 1–3, 1–4, 2–5, 2–11, 2–13, 2–16, 2–17, 2–21 and 2–44) were produced by US Department of Agriculture-Agricultural Research Center (USDA-ARS, Peoria, IL, USA) and have been described in Maragos et al. [29 (link)]. A Mab specific for T-2 (clone 1) was produced by Chinese Academy of Agricultural Sciences—Oil Crops Research Institute (Wuhan, China) and has been described by Zhang et al. [35 (link)]. A Mab specific for HT-2 (clone H10-A10) was purchased from University of Natural Resources and Life Science of Vienna—Department for Agrobiotechnology IFA-Tulln (Tulln, Austria). T-2 glucoside and HT-2 glucoside, both as α-anomers, were produced by USDA-ARS and have been described by McCormick et al. [36 (link)]. Glass culture tubes (10 × 75 mm) were obtained from VWR International (Milan, Italy). Paper filters (No. 4) and glass microfiber filters (GF/A) were purchased from Whatman (Maidstone, UK).

Acetonitrile and methanol (both HPLC grade) were purchased from Carlo Erba Reagents (Milan, Italy). Dichloromethane and hydrochloric acid (both HPLC grade) were purchased from Mallinckrodt Baker (Milan, Italy). Ultrapure water was produced by a Millipore Milli-Q system (Millipore, Bedford, MA). Ochratoxin A (OTA), sodium chloride (NaCl, ACS grade), sodium hydrogen carbonate (NaHCO₃, ACS grade), Tween 20, and acetic acid were purchased from Sigma-Aldrich (Milan, Italy). OTA immunoaffinity columns (OchraTest™) were obtained from VICAM, A Waters Business (Milford, MA). Glass microfiber filters (GF/A) and paper filters (No. 4) were purchased from Whatman (Maidstone, UK). OTA immunoaffinity columns (OchraPrep^®) and ELISA test kits (RIDASCREEN^® Ochratoxin A 30/15) were purchased from R-Biopharm (R-Biopharm AG, Darmstadt, Germany).

Analytical-grade acetonitrile (ACN), methanol (MeOH), and toluene (for HPLC purpose) were purchased from Mallinckrodt Baker (Milan, Italy). Ultrapure water was produced by a Millipore Milli-Q system (Millipore, Bedford, MA, USA). Filter paper and Glass microfiber filters (GF/A) were purchased from Whatman (Maidstone, UK).
Standard of aflatoxin B₁, 2-azino-di-[3-ethylbenzo-thiazolin-sulphonate] (ABTS), syringaldehyde, and acetosyringone were obtained from Sigma Aldrich (Milan, Italy). Immunoaffinity columns AflaTest^® Wide Bore were obtained from Vicam L.P. (Watertown, MA, USA).
Organic corn kernels (Zea Mais L.) were purchased from Bioseme s.c.a.r.l.