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Geltrex reduced growth factor matrix

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Geltrex Reduced Growth-Factor Matrix is a soluble basement membrane extract of proteins derived from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is used as a cell culture substrate to support the growth and differentiation of various cell types, including stem cells, primary cells, and cell lines.

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Lab products found in correlation

Eclipse ts100 microscope, by Nikon (2 mentions) Ds fi1, by Nikon (2 mentions)

Spelling variants of this product

Geltrex (760 citations) Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix (92 citations) Geltrex matrix (68 citations) Geltrex: Reduced Growth Factor Basement Membrane Matrix (14 citations) Geltrex LDEV-Free hESC-qualified Reduced Growth Factor Basement Membrane Matrix (11 citations) Reduced Growth Factor GelTrex (7 citations)

2 protocols using geltrex reduced growth factor matrix

1

HUVEC Tube Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVECs transfected with the corresponding siRNA were plated onto Geltrex Reduced Growth-Factor Matrix (Invitrogen) in Medium 200 with no phenol red and supplemented with 2% foetal calf serum and VEGF (25 ng/ml) when indicated. Tube formation was quantified after incubation at 37 °C for 16 h. Images were recorded with a Nikon DSFi1 digital camera coupled to a Nikon ECLIPSE TS100 microscope at 4× magnification.
Savage A.M., Kurusamy S., Chen Y., Jiang Z., Chhabria K., MacDonald R.B., Kim H.R., Wilson H.L., van Eeden F.J., Armesilla A.L., Chico T.J, & Wilkinson R.N. (2019). tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis. Nature Communications, 10, 732.
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2

HUVECs Tube Formation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVECs were plated onto Geltrex Reduced Growth-Factor Matrix (Invitrogen) in Medium 200 with no phenol red and supplemented with 2% fetal calf serum and 50 ng/ml growth factor (VEGF or bFGF) in the presence of ATA at the indicated concentration or DMSO as a control. Tube formation was quantified after incubation at 37 °C for 16 h. Images were recorded with a Nikon DSFi1 digital camera coupled to a Nikon ECLIPSE TS100 microscope at 4× magnification.
Kurusamy S., López-Maderuelo D., Little R., Cadagan D., Savage A.M., Ihugba J.C., Baggott R.R., Rowther F.B., Martínez-Martínez S., Arco P.G., Murcott C., Wang W., Francisco Nistal J., Oceandy D., Neyses L., Wilkinson R.N., Cartwright E.J., Redondo J.M, & Armesilla A.L. (2017). Selective inhibition of plasma membrane calcium ATPase 4 improves angiogenesis and vascular reperfusion. Journal of molecular and cellular cardiology, 109.
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