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Tri carb liquid scintillation analyzer

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The Tri-Carb Liquid Scintillation Analyzer is a laboratory instrument designed for the detection and measurement of radioactive samples. It utilizes liquid scintillation counting technology to quantify the level of radioactivity in liquid samples.

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Lab products found in correlation

Ultima gold f scintillation fluid, by PerkinElmer (2 mentions) U 14c palmitate, by PerkinElmer (1 mentions) Phenethylamine, by Merck Group (1 mentions) 1 14c acetic acid, by PerkinElmer (1 mentions) 1 14c acetate, by PerkinElmer (1 mentions) Cytoscint, by MP Biomedicals (1 mentions) Gf c filter, by Cytiva (1 mentions) 1 14c acetic acid, by American Radiolabeled Chemicals (1 mentions)

Close spelling variants of this product

Tri-Carb 2300 TR liquid scintillation analyzer TRI-CARB 2000 CA Liquid Scintillation Analyzer Tri-Carb 2100TR Liquid Scintillation Analyzer Tri-Carb 2900 TR Liquid Scintillation Analyzer Liquid scintillation analyzer

7 protocols using tri carb liquid scintillation analyzer

1

Fatty Acid Oxidation in TSC2-Deficient Cells

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TSC2 add-back (300,000/well for 24 hr and 150,000/well for 72 hr) and TSC2-deficient cells (200,000/well for 24 hr and 100,000 for 72 hr) were seeded in 12-well plates and treated with GLPG1690 (6 μM) or control (0.06% DMSO) in DMEM with 10% FBS for 24 or 72 hr. Cells were then incubated for 3 hr at 37ºC with 1 μCi/mL of [U-14C]palmitate (PerkinElmer Inc., MA). 3 M perchloric acid was added to the cell culture medium and the wells were sealed with Whatman filter paper saturated with phenethylamine (Sigma-Aldrich) to capture 14C-CO2. The plates were gently shaken for 3 hr at room temperature and the filter paper was removed and placed into Ultima Gold F Scintillation Fluid (PerkinElmer Inc.). Radioactivity was counted on a Packard Tri-Carb Liquid Scintillation Analyzer. Data were normalized against the protein mass (total μg from three independent wells).
Feng Y., Mischler W.J., Gurung A.C., Kavanagh T.R., Androsov G., Sadow P.M., Herbert Z.T, & Priolo C. (2020). Therapeutic targeting of the secreted lysophospholipase D autotaxin suppresses tuberous sclerosis complex-associated tumorigenesis. Cancer research, 80(13), 2751-2763.
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2

Lipid Synthesis in TSC2-Deficient Cells

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Human TSC2-deficient cells (400,000/well for 24 hr and 200,000 for 72 hr) and TSC2 add-back cells (600,000/well for 24 hr and 300,000/well for 72 hr) were seeded in 6-well plates and treated with GLPG1690 (6 μM) or control (0.06% DMSO) in DMEM with 10% FBS for 24 or 72 hr. Cells were then labeled with [1-14C]acetic acid (0.5 μCi/ml; PerkinElmer, MA) for 4 hr, washed 2 times with PBS and collected for lipid extraction using isopropanol (500 μL). Radioactivity from 20 μL of the lipid extract was counted on a Packard Tri-Carb Liquid Scintillation Analyzer. Data were normalized against the protein mass (total μg from three independent wells).
Feng Y., Mischler W.J., Gurung A.C., Kavanagh T.R., Androsov G., Sadow P.M., Herbert Z.T, & Priolo C. (2020). Therapeutic targeting of the secreted lysophospholipase D autotaxin suppresses tuberous sclerosis complex-associated tumorigenesis. Cancer research, 80(13), 2751-2763.
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3

Measuring Inositol Phosphate Signaling

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24h post-transfection, transiently transfected HEK293T cells were seeded in poly-L-lysine coated 48 well plates and labeled overnight with myo-[2-3H]-inositol (1 μCi/ml) in Earle's inositol-free minimal essential medium supplemented with 10% FBS and 1% P/S. The next day, cells were washed with buffer (20 mM HEPES, 140 mM NaCl, 5 mM KCl, 1 mM MgSO4, 1 mM CaCl2, 10 mM glucose) supplemented with 0.1% BSA and incubated for 2h in the above mentioned buffer containing 10 mM LiCl in the absence or presence of indicated concentrations chemokines. Incubation was terminated by placing the cells on ice and aspirating the stimulation buffer prior to the addition of ice-cold 10 mM formic acid. After incubating the formic acid for 90 min on ice, generated [3H]-inositol phosphates (InsP) were isolated by anion-exchange chromatography (Dowex AG1-X8 columns; Bio-Rad) and counted by a Packard Tri-Carb liquid scintillation analyzer.
de Munnik S.M., Kooistra A.J., van Offenbeek J., Nijmeijer S., de Graaf C., Smit M.J., Leurs R, & Vischer H.F. (2015). The Viral G Protein-Coupled Receptor ORF74 Hijacks β-Arrestins for Endocytic Trafficking in Response to Human Chemokines. PLoS ONE, 10(4), e0124486.
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4

Measuring Cellular Acetate Metabolism

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Cells were seeded in 12-well plates, treated with rapamycin (20 nM) for 24 hr, and incubated for 3 hours at 37°C with 1 μCi/mL of [1-14C]acetate (PerkinElmer Inc., MA). Three mol/L of perchloric acid was added to the culture media and the dishes were sealed with a phenylethylamine (Sigma-Aldrich)-saturated Whatman filter paper to capture 14C-CO2, as previously done 32 (link). Following 3-hour incubation at room temperature on a gentle shaker, the filter paper was removed, placed into Ultima Gold F Scintillation Fluid (PerkinElmer Inc.), and radioactivity was counted on a Packard Tri-Carb Liquid Scintillation Analyzer.
Verwer E.E., Kavanagh T.R., Mischler W.J., Feng Y., Takahashi K., Wang S., Shoup T.M., Neelamegam R., Yang J., Guehl N.J., Ran C., Massefski W., Cui Y., El-Chemaly S., Sadow P.M., Oldham W.M., Kijewski M.F., El Fakhri G., Normandin M.D, & Priolo C. (2018). [18F]Fluorocholine and [18F]fluoroacetate PET as imaging biomarkers to assess phosphatidylcholine and mitochondrial metabolism in preclinical models of TSC and LAM. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(23), 5925-5938.
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5

Binding Assay for [3H]PK 11195

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Binding assays of [3H]PK 11195, including displacement studies were done as previously described.76 (link) The reaction mixtures for the binding assays contained 400 μl of homogenized rat kidney membranes (5 mg of homogenate/ml) and 25 μl of [3H]PK 11195 (2 nM final concentration)76 (link) in the absence (total binding) or presence of various concentrations (10-10 M–10-5 M) of the synthesized compounds described in this study. After incubation for 60 min at 4 °C, samples were filtered under vacuum over Whatman GF/C filters and washed three times with 5 ml of 50 mM Tris-HCl buffer, pH 7.4. The filters were incubated in CytoScint (MP Biomedicals) and radioactivity was measured after 12 h with a 1600 CA Tri-Carb liquid scintillation analyzer (Packard). Inhibitory constant (Ki) values were calculated by the equation Ki=IC50/(1+C/Kd), where C=[3H] PK 11195 concentration, IC50=concentration causing 50% inhibition of [3H]PK 11195 binding and Kd=2 nM (from Scatchard analysis of [3H]PK 11195 binding to kidney membranes).
Vainshtein A., Veenman L., Shterenberg A., Singh S., Masarwa A., Dutta B., Island B., Tsoglin E., Levin E., Leschiner S., Maniv I., Pe’er L., Otradnov I., Zubedat S., Aga-Mizrachi S., Weizman A., Avital A., Marek I, & Gavish M. (2015). Quinazoline-based tricyclic compounds that regulate programmed cell death, induce neuronal differentiation, and are curative in animal models for excitotoxicity and hereditary brain disease. Cell Death Discovery, 1, 15027-.
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6

Lipid Labeling and Extraction in ELT3 Cells

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ELT3 cells were labeled with [1-14C]acetic acid or [1-14C]FACE (0.5 μCi /ml) obtained from American Radiolabeled Chemicals (ARC, MO) for 6 hr, then washed 3 times and collected for lipid extraction using methanol/chloroform/H2O (2:1:0.5 v/v/v). Radioactivity was counted on a Packard Tri-Carb Liquid Scintillation Analyzer.
Verwer E.E., Kavanagh T.R., Mischler W.J., Feng Y., Takahashi K., Wang S., Shoup T.M., Neelamegam R., Yang J., Guehl N.J., Ran C., Massefski W., Cui Y., El-Chemaly S., Sadow P.M., Oldham W.M., Kijewski M.F., El Fakhri G., Normandin M.D, & Priolo C. (2018). [18F]Fluorocholine and [18F]fluoroacetate PET as imaging biomarkers to assess phosphatidylcholine and mitochondrial metabolism in preclinical models of TSC and LAM. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(23), 5925-5938.
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7

Measuring Fatty Acid Synthesis in ELT3 Cells

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ELT3 cells were seeded in 12-well plates (50,000 cells/well) in DMEM supplemented with 10% FBS, treated with rapamycin (20 nM) or vehicle (DMSO) for 24 hours, labeled with [1-14C]FACE (1 μCi/ml) for 6 hr, carefully washed three times with PBS, and collected for radioactivity count on a Packard Tri-Carb Liquid Scintillation Analyzer.
Verwer E.E., Kavanagh T.R., Mischler W.J., Feng Y., Takahashi K., Wang S., Shoup T.M., Neelamegam R., Yang J., Guehl N.J., Ran C., Massefski W., Cui Y., El-Chemaly S., Sadow P.M., Oldham W.M., Kijewski M.F., El Fakhri G., Normandin M.D, & Priolo C. (2018). [18F]Fluorocholine and [18F]fluoroacetate PET as imaging biomarkers to assess phosphatidylcholine and mitochondrial metabolism in preclinical models of TSC and LAM. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(23), 5925-5938.
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