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Uv 1750 spectrometer

Manufactured by Shimadzu
Sourced in Japan

The UV-1750 spectrometer is a laboratory instrument designed for the analysis of samples using ultraviolet and visible light spectroscopy. It is capable of measuring the absorbance or transmittance of light through a sample across a specified range of wavelengths.

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7 protocols using uv 1750 spectrometer

1

UV-Vis and Photoluminescence Spectroscopy

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UV–visible absorption spectra were recorded on a SHIMADZU UV-1750 spectrometer. Photoluminescence (PL) spectra were measured using a Horiba JobinYvon FluoroLog-3 spectrometer platform.
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2

UV-vis and Fluorescence Spectroscopy of Solutions

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UV-vis absorption spectra in solution (1 × 10–5 mol L−1) in a 1 cm quarts cuvette were collected on a Shimadzu UV-1750 spectrometer at resolution of 1.0 nm. Fluorescence spectra in solution were recorded on a Horiba Jobin Yvon spectrometer (Nanolog FL3-2iHR). The luminescence quantum yield in solution was measured using rhodamine 6G (under excitation of 488 nm, Φf = 0.88 in ethanol) as reference. The quantum yield Φ is calculated using the equation: Φu = [(AsFun2)/ (AuFsn02)]Φs, where As and Au are the absorbance of the reference (or standard) and sample (or unknown) solutions at their respective excitation wavelengths, Fs and Fu are the corresponding integrated fluorescence intensity, and n and n0 are the refractive indexes of the solvents of the sample and the reference, respectively39 (link).
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3

Characterization of L1013 Nanoparticles

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Nuclear magnetic resonance (NMR) spectra were recorded on a Bruker Avance III 400 MHz NMR spectrometer (400 MHz for 1H, referenced to TMS at δ =0.00 ppm and 100 MHz for 13C, referenced to CDCl3 at 77.0 ppm). The hydrodynamic diameter and zeta potential of L1013 NPs were recorded on Micromeritics Nanoplus-3 (US). Transmission electron microscopy (TEM) images were obtained on a JEOL JEM-2100 electron microscope with an accelerating voltage of 200 KV. UV-vis-NIR spectra were measured on a Shimadzu UV-1750 spectrometer. Photoluminescence (PL) spectra were recorded on an Edinburgh instruments FLS980, using Xe lamp as the excitation source and a liquid nitrogen cooled InGaAs diode detector for signal detection. Fluorescence quantum yield was determined using IR26 in 1,2-dichloroethane (0.5%) as the standard. The absorbance of solutions was controlled below 0.1 to avoid internal filter effect.
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4

Spectroscopic Characterization of BisBDP Nanoparticles

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UV-vis and UV-vis–NIR absorption spectra were respectively measured at room temperature on a Shimadzu UV-1750 spectrometer and a Shimadzu UV-3600 spectrophotometer with a resolution of 1.0 nm, using quartz cuvettes of 1.0-cm path length. The full absorbance spectrum of BisBDP2 NPs in PBS (300 to 1600 nm) was obtained by using quartz cuvettes of 0.5-cm path length. Fluorescence spectra of BDP1 were measured on a FLUOROMAX-4 spectrometer under an air atmosphere at room temperature; Fluorescent spectra of BDP2 and BisBDP1 were recorded using an Edinburgh FLSP920 fluorescence spectrophotometer equipped with a microsecond flash-lamp (UF900). NIR-II fluorescence spectra of BisBDP2 were recorded on a three-dimensional fluorescence spectrometer at room temperature.
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5

Characterization of PBD-CD36 Nanoparticles

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The hydrodynamic diameter and zeta potential of PBD-CD36 NPs were recorded on Micromeritics Nanoplus-3. Transmission electron microscopy (TEM) images were measured on a JEOL JEM-2100 electron microscope with an accelerating voltage of 200 kV. UV-vis-NIR spectra were measured on a Shimadzu UV-1750 spectrometer.
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6

Spectroscopic Characterization of BisBDP Nanoparticles

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UV-vis and UV-vis–NIR absorption spectra were respectively measured at room temperature on a Shimadzu UV-1750 spectrometer and a Shimadzu UV-3600 spectrophotometer with a resolution of 1.0 nm, using quartz cuvettes of 1.0-cm path length. The full absorbance spectrum of BisBDP2 NPs in PBS (300 to 1600 nm) was obtained by using quartz cuvettes of 0.5-cm path length. Fluorescence spectra of BDP1 were measured on a FLUOROMAX-4 spectrometer under an air atmosphere at room temperature; Fluorescent spectra of BDP2 and BisBDP1 were recorded using an Edinburgh FLSP920 fluorescence spectrophotometer equipped with a microsecond flash-lamp (UF900). NIR-II fluorescence spectra of BisBDP2 were recorded on a three-dimensional fluorescence spectrometer at room temperature.
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7

Quantifying Phytochemicals in Apple Seedlings

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For the determination of H2O2, enzyme activity, and phytohormones, nine apple seedlings were selected for each experimental replicate (n = 3) to provide an adequate amount of root and leaf tissue. The levels of hydrogen peroxide (H2O2, SO1300), and the activities of peroxidase (POD, KT5058), catalase (CAT, KT4957), abscisic acid (ABA, KT4924), and indoleacetic acid (IAA, NR, KT4992) were determined using commercial test kits purchased from Jiangsu Kete Biotechnology Co., Ltd. (Jiangsu, China). H2O2 was recorded on a UV-1750 spectrometer (Shimadzu, Japan). The enzyme activities were analyzed using an ELISA reader (Multiskan MS, Labsystems 325, Helsinki, Finland).
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