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4 oh tamoxifen tam

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4-OH tamoxifen (Tam) is a laboratory compound used for research purposes. It is a metabolite of the drug tamoxifen, which is commonly used in the treatment of certain types of breast cancer. The core function of 4-OH tamoxifen is to serve as a research tool in studies related to tamoxifen and its biological effects.

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Lab products found in correlation

17β estradiol e2, by Merck Group (2 mentions) Cyclin d1, by Thermo Fisher Scientific (2 mentions) Rabbit anti ccdc170, by GeneTex (2 mentions) Mouse anti cmet and rabbit anti gab1, by Cell Signaling Technology (2 mentions) Charcoal stripped fetal calf serum, by Merck Group (1 mentions) Torin1, by Merck Group (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Chloroquine cq, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Trehalose, by Merck Group (1 mentions) Bafilomycin a1 bafa1, by Merck Group (1 mentions)

Close spelling variants of this product

Tamoxifen (3381 citations) 4-OH tamoxifen (110 citations) Tamoxifen (TAM, (101 citations) OH-TAM (5 citations) 4-hydroxy-tamoxifen (OH-Tam, (4 citations)

4 protocols using 4 oh tamoxifen tam

1

Establishing Tamoxifen-Resistant Breast Cancer Cells

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MCF-7/TAM and T47D/TAM breast cancer cells resistant to tamoxifen were established according to the reported method (53 (link)). In brief, MCF-7 and T47D were seeded and cultured for 24 h and then changed media to 5% charcoal-stripped fetal calf serum (Sigma-Aldrich) in phenol red-free DMEM and RPMI1640 medium, respectively, and culture for 24 h. Then 0.1 μM 4-OH tamoxifen (TAM) (Sigma-Aldrich) was added to the medium. The cells were continuously treated with gradually increasing doses of TAM for 8 months. MCF-7/TAM and T47D/TAM TamR breast cancer cells were finally obtained when cells can tolerate 1 μM TAM.
Zhang S., Wang R., Wang X., Guo X., Du Y., Guo X., Zong X., Zhu C, & Zhou X. (2022). HBXIP is a novel regulator of the unfolded protein response that sustains tamoxifen resistance in ER+ breast cancer. The Journal of Biological Chemistry, 298(3), 101644.
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2

Protein Detection in Serum-Depleted Cells

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Protein samples were separated in SDS-PAGE gel and transferred either onto 0.2μm PVDF membrane for detection of the E2-E10 protein product, or 0.2μm nitrocellulose membrane for other proteins. The dilutions of primary antibodies used were 1:250-1:1000 for rabbit anti-CCDC170 (GeneTex), 1:1000 for mouse anti-cMet and rabbit anti-Gab1 (Cell Signaling) and other antibodies. Antibodies were obtained from Santa Cruz (cyclin D1), Thermo Fisher (ERα), Abcam (PAK1), Millipore (Src), and Cell Signaling (all other molecules). To study the fusion-driven signaling in the condition of serum withdrawal and endocrine treatment, cells were maintained in phenol red-free medium for 48h, serum-starved for 24h, and then treated for 20 minutes with vehicle (Ethanol), 17β-estradiol (E2) (1nM) or Tam (100nM). 4-OH tamoxifen (Tam) and 17β-estradiol (E2) were obtained from Sigma-Aldrich.
Veeraraghavan J., Tan Y., Cao X.X., Kim J.A., Wang X., Chamness G.C., Maiti S.N., Cooper L.J., Edwards D.P., Contreras A., Hilsenbeck S.G., Chang E.C., Schiff R, & Wang X.S. (2014). Recurrent ESR1-CCDC170 rearrangements in an aggressive subset of estrogen-receptor positive breast cancers. Nature communications, 5, 4577.
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3

Protein Detection in Serum-Depleted Cells

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Protein samples were separated in SDS-PAGE gel and transferred either onto 0.2μm PVDF membrane for detection of the E2-E10 protein product, or 0.2μm nitrocellulose membrane for other proteins. The dilutions of primary antibodies used were 1:250-1:1000 for rabbit anti-CCDC170 (GeneTex), 1:1000 for mouse anti-cMet and rabbit anti-Gab1 (Cell Signaling) and other antibodies. Antibodies were obtained from Santa Cruz (cyclin D1), Thermo Fisher (ERα), Abcam (PAK1), Millipore (Src), and Cell Signaling (all other molecules). To study the fusion-driven signaling in the condition of serum withdrawal and endocrine treatment, cells were maintained in phenol red-free medium for 48h, serum-starved for 24h, and then treated for 20 minutes with vehicle (Ethanol), 17β-estradiol (E2) (1nM) or Tam (100nM). 4-OH tamoxifen (Tam) and 17β-estradiol (E2) were obtained from Sigma-Aldrich.
Veeraraghavan J., Tan Y., Cao X.X., Kim J.A., Wang X., Chamness G.C., Maiti S.N., Cooper L.J., Edwards D.P., Contreras A., Hilsenbeck S.G., Chang E.C., Schiff R, & Wang X.S. (2014). Recurrent ESR1-CCDC170 rearrangements in an aggressive subset of estrogen-receptor positive breast cancers. Nature communications, 5, 4577.
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4

Lysosomal Modulation and Autophagy Assays

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Chemical reagents including Lysotracker red DND-99 (Life Technologies, USA), 4,6-diamidino-2-phenylindole (DAPI), trehalose, torin 1, chloroquine (CQ), bafilomycin A1 (BafA1), and 4-OH-tamoxifen (Tam) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The restriction enzymes used in these experiments were purchased from New England BioLabs, Inc. (Beverly, MA). Antibodies used in this work are listed in Supplementary Table S2.
Wang L., Sun J., Yin Y., Sun Y., Ma J., Zhou R., Chang X., Li D., Yao Z., Tian S., Zhang K., Liu Z, & Ma Z. (2021). Transcriptional coregualtor NUPR1 maintains tamoxifen resistance in breast cancer cells. Cell Death & Disease, 12(2), 149.
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