Data logger

The experiment was conducted in a chapel type greenhouse from October 2015 to March 2016 (28 m altitude, 24°44′02″ N and 107°27′16″ W). Six cultivars of H. sabdariffa: ‘Cruza Negra’ (‘Cruza N ’), ‘UAN16-2’, ‘Criolla Huajicori’ (‘Criolla H.’), ‘UAN 6 Puga’, ‘UAN 25-1’ and ‘4Q4’) were studied, as provided by the Autonomous University of Nayarit, México. All cultivars were grown under hydroponic conditions. Within the greenhouse, a Hobo data logger was placed over the canopy of the plants to record daily temperatures and relative humidity (average maximum and minimum temperature of 23.95 and 20.18 °C, respectively, and relative humidity of 71.49%). The seeds were planted in 120-well germination trays in a peat moss–agrolite mixture (70:30 v/v). At 14 days after germination, the seedlings were transplanted to a greenhouse in a completely random arrangement, and each experimental unit represented four plants and four experimental replicates for each cultivar. Each seedling was placed in 13 L polyethylene bags of substrate (60% fluvisol soil and 40% coconut fiber). The distance between bags was 0.50 m and between rows was 1.0 m. A Steiner solution [55 (link)] with an EC of 2 dS m⁻¹ and pH of 5.8 was provided by drip irrigation at a rate of eight daily events of 5 min⁻¹ using 4 L h⁻¹ drippers.

The trials to compare the energy required to gin the fuzzless-tufted (one of the BC₇F₅ NILs) and normal fuzzy (G. hirsutum, cv. Sicot 71BRF) seed cotton were conducted at CSIRO Manufacturing (Geelong, Victoria, Australia). Trials were done using a single stand, cut down (119 to 40 saws) industrial scale Continental Eagle saw gin stand with a Continental/Moss Gordin Galaxie feeder with a maximum output of just under two bales/hr (400 kg/h). Two gin saw motor speeds (slow: 35 Hz/500 rpm and fast: 43 Hz/600 rpm) were assessed. Speeds were modulated using an ABB variable frequency drive attached to the 50 kW gin saw motor. No lint cleaning was used. For each genotype, three ginning runs (50 kg/run) were conducted under each motor speed. Current (ampere) was measured using a clamp on ammeter to one of the three-phase lines to the gin motor. Data was recorded using a HOBO data-logger at a frequency of one Hz. Measured amperes were converted to power values (kW) using the peak voltage (415 V) for a single phase and assuming a power factor of one. The kilowatt hours (kWh) for each run were determined by multiplying the time to gin 50 kg seed cotton by the average kW value for the run. All ginning runs were carried out under warm, dry ambient conditions (18–23°C and 30–50% relative humidity) with the fiber moisture content <5.5%.

Eight hour low humidity and moderate humidity exposure were conducted in a specially fabricated environmental chamber. The chamber interior was segmented into three similar compartments, each with dimensions approximately 61 cm × 61 cm × 46 cm (Fig. 1a, b). Two compartments were sealed to limit the influx of room air and were intended for low humidity exposure, whereas the third compartment was left open to room air and was intended for a moderate humidity control. Gated duct caps were included within the wall of the low humidity compartment to allow for titration of room air as necessary.
Low humidity was achieved with a 70-pint commercial dehumidifier (Hisense DH70K1G: Qingdao, China) set to High Continuous attached to the chamber via 4-in. ducting. Moderate humidity exposure was achieved by opening the chamber airspace to room air without conditioning from the dehumidifier. Internal relative humidity and temperature were tracked using a HOBO Data Logger with a 12-bit Temperature/Relative Humidity Smart Sensor (U14–002, S-THB-M002: ONSET, Bourne, MA) at one-minute intervals.

A survey of the Ae. albopictus that breeds in containers was performed in order to ascertain their current distribution. The collection sites were located all along the National Route 12, the major roadway that connects Posadas to Puerto Iguazú, both located in the Misiones province, in Subtropical Argentina, in the northern part of the country (Fig. 1). All collections were taken from various artificial containers of standing water in the area using a siphon bottle. For Iguazú National Park, which belongs to Parques Nacionales, Argentina, a NEA 326 permit was obtained. Morphological identification of the specimens (3^rd and 4^th instar larvae) was performed using dichotomous keys⁴⁸ , while 1^st and 2^nd instars and pupae were reared either to the 4^th instar or to adult emergence, respectively. Immature stages were transported to the lab forfurther processing. Daily temperature and humidity were recorded by hour using a HOBO data logger during February in Iguazú National Park, where we were able to collect a sample of Ae. albopictus. These data were used to build an average curve of maximum to minimum daily cycle of temperature from this location.

The health and welfare of the detection dog were critical to the success of this study. The detection dog was under the care of the Disney’s Animals, Science and Environment veterinarian team. We took into consideration the physical welfare of the detection dog at all times, ensuring the dog was always properly hydrated and had access to shade and a cooling fan. Signals regularly monitored for heat exhaustion included gum discoloration, excessive panting, and lethargy. We outfitted the ATV used to transport the detection dog with a soft crate to allow air circulation, a cooling mat inside the crate, a fan to increase airflow, and an adjustable umbrella to keep the detection dog in the shade during transportation. A Hobo data logger on the collar of the detection dog monitored the temperature every 30 minutes during the time the detection dog was working, and sand surface temperature was recorded at each sea turtle crawl location. Our protocol was to remove the detection dog from the beach immediately and take her to an air-conditioned building should there be any sign of critical heat stress, and to leave the beach if she no longer showed interest in working.