Monolayers of 3 × 105 A549 cells were infected with A. baumannii at a MOI of 100. After infection, the cells were washed and incubated by additional 24 h in DMEM supplemented with gentamicin 100 μg/mL. At 24 h post-infection, the supernatants were used to quantify the presence of TNFα, IL-6, IL-1β by QuantiGlo® ELISA (R&D Systems). The relative light units (RLUs) were determined in a luminometer (Flouroskan Ascent FL by Thermo Fisher®), during 1 min, lag time, 0.5 s/well read in an auto gain mode. The data was analyzed with the Ascent Software version 2.4.
Quantiglo elisa
Manufactured by R&D Systems
Sourced in United Kingdom
The QuantiGlo ELISA is a chemiluminescent enzyme-linked immunosorbent assay (ELISA) system used for the quantitative measurement of target analytes in biological samples. The assay utilizes a luminol-based substrate that produces light upon catalytic reaction with a specific enzyme label, allowing for sensitive detection and quantification of the target molecule.
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Lab products found in correlation
5 protocols using quantiglo elisa
1
Quantification of Cytokine Secretion in A549 Cells Infected with A. baumannii
2
Plasma Biomarker Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The concentrations of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor alpha (TNF-α), endothelin-1, and E-Selectin in the plasma samples were determined by enzyme immunoassay (EIA) using commercial reagents: IL-1β (sensitivity 0.063 pg/ml) and TNF-α (sensitivity 0.125 pg/ml), Quantikine HS ELISA, R&D Systems Europe Ltd., Abindgon, UK; IL-6 (sensitivity 0.6 pg/ml), PeliPair ELISA, Sanquin, Amsterdam, the Netherlands; IL-8 (sensitivity 1.56 pg/ml), Opt EIA, BD Biosciences, Erembodegem, Belgium; endothelin-1 (sensitivity 0.68 pg/ml), QuantiGlo ELISA, R&D Systems Europe Ltd., Abindgon, UK; E-Selectin (sensitivity 20.5 pg/ml), ELISA, HyCult Biotechnology, Uden, the Netherlands).
Platelet count, leucocytes and their differential count, hemoglobin, haematocrit, sensitive C-reactive protein (CRP), lipids, glucose, and levels of fibrinogen were analyzed using established methods.
All laboratory analyses were performed blind to the exposure status of the studied participants.
Platelet count, leucocytes and their differential count, hemoglobin, haematocrit, sensitive C-reactive protein (CRP), lipids, glucose, and levels of fibrinogen were analyzed using established methods.
All laboratory analyses were performed blind to the exposure status of the studied participants.
3
Quantitative Endothelin-1 Measurement
4
Quantification of ET-1 Levels
5
Quantifying Endothelin-1 Levels in BMVEC Cultures
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