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Reprosil pur 120 c18 aq 2.4 μm beads

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ReproSil-Pur 120 C18-AQ 2.4 μm beads are a type of chromatography media used for high-performance liquid chromatography (HPLC) and other analytical techniques. The beads are composed of spherical silica particles with a chemically bonded C18 (octadecyl) stationary phase. They have an average particle size of 2.4 μm and a pore size of 120 Å, which makes them suitable for a wide range of analytical applications.

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Lab products found in correlation

Q exactive hf x quadrupole orbitrap mass spectrometer, by Thermo Fisher Scientific (5 mentions) Easy nlc 1200 nano uhplc, by Thermo Fisher Scientific (4 mentions) Easy nlc1200 nano, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ReproSil-Pur C18-AQ (72 citations) ReproSil-Pur 120 C18-AQ (58 citations) ReproSil-Pur C18 beads (32 citations) C18 beads (23 citations) Reprosil-Pur C18-AQ beads (21 citations) Reprosil-Pur C18 (21 citations) Reprosil-AQ Pur (3 citations) ReproSil-Pur 120 C18-AQ 2.4 μm (2 citations)

5 protocols using reprosil pur 120 c18 aq 2.4 μm beads

1

Peptide Analysis by LC-MS/MS

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Peptides were resuspended in water with 0.1 % formic acid (FA) and analyzed using EASY-nLC 1200 nano-UHPLC coupled to Q Exactive HF-X Quadrupole-Orbitrap mass spectrometer (Thermo Scientific). The chromatography column consisted of a 40 cm long, 75 μm i.d. microcapillary capped by a 5 μm tip and packed with ReproSil-Pur 120 C18-AQ 2.4 μm beads (Dr. Maisch GmbH). LC solvents were 0.1 % FA in H2O (Buffer A) and 0.1 % FA in 90 % MeCN: 10 % H2O (Buffer B). Peptides were eluted into the mass spectrometer at a flow rate of 300 nL/min. over a 240 min. linear gradient (5–35 % Buffer B) at 65°C. Data were acquired in data-dependent mode (top-20, NCE 28, R = 7’500) after full MS scan (R = 60’000, m/z 400–1’300). Dynamic exclusion was set to 10 s, peptide match to prefer and isotope exclusion was enabled.
Dickson P., Abegg D., Vinogradova E., Takaya J., An H., Simanski S., Cravatt B.F., Adibekian A, & Kodadek T. (2020). Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell chemical biology, 27(11), 1371-1382.e6.
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2

Nano-UHPLC-MS/MS Peptide Analysis

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Peptides were resuspended in water with 0.1% formic acid (FA) and analyzed using EASY-nLC 1200 nano-UHPLC coupled to Q Exactive HF-X Quadrupole-Orbitrap mass spectrometer (Thermo Scientific). The chromatography column consisted of a 50 cm long, 75 μm i.d. microcapillary capped by a 5 μm tip and packed with ReproSil-Pur 120 C18-AQ 2.4 μm beads (Dr. Maisch GmbH). LC solvents were 0.1% FA in H2O (Buffer A) and 0.1% FA in 90% MeCN: 10% H2O (Buffer B). Peptides were eluted into the mass spectrometer at a flow rate of 300 nL/min over a 240 min linear gradient (5-35% Buffer B) at 65°C. Data were acquired in data-dependent mode (top-20, NCE 28, R = 7,500) after full MS scan (R = 60,000, m/z 400-1,300). Dynamic exclusion was set to 10 s, peptide match to prefer, and isotope exclusion was enabled.
Haniff H.S., Liu X., Tong Y., Meyer S.M., Knerr L., Lemurell M., Abegg D., Aikawa H., Adibekian A, & Disney M.D. (2021). A structure-specific small molecule inhibits a miRNA-200 family member precursor and reverses a type 2 diabetes phenotype. Cell chemical biology, 29(2), 300-311.e10.
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3

Peptide Analysis by Nano-UHPLC-MS

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Peptides were resuspended in water with 0.1% formic acid (FA) and analyzed using EASY-nLC 1200 nano-UHPLC coupled to Q Exactive HF-X Quadrupole-Orbitrap mass spectrometer (Thermo Scientific). The chromatography column consisted of a 30 cm long, 75 μm i.d. microcapillary capped by a 5 μm tip and packed with ReproSil-Pur 120 C18-AQ 2.4 μm beads (Dr. Maisch GmbH). LC solvents were 0.1% FA in H2O (Buffer A) and 0.1% FA in 90% MeCN:10% H2O (Buffer B). Peptides were eluted into the mass spectrometer at a flow rate of 300 nL/min over a 240 min linear gradient (5–35% Buffer B) at 65 °C. Data were acquired in data-dependent mode (top-20, NCE 28, R = 7500) after full MS scan (R = 60000, m/z 400–1300). Dynamic exclusion was set to 10 s, peptide match set to prefer, and isotope exclusion was enabled.
Costales M.G., Hoch D.G., Abegg D., Childs-Disney J.L., Velagapudi S.P., Adibekian A, & Disney M.D. (2019). A Designed Small Molecule Inhibitor of a Non-Coding RNA Sensitizes HER2 Negative Cancers to Herceptin. Journal of the American Chemical Society, 141(7), 2960-2974.
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4

Nano-UHPLC-MS/MS Peptide Analysis

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Peptides were resuspended in water with 0.1 % FA and analyzed using an EASY-nLC 1200 nano-UHPLC coupled to a Q Exactive HF-X Quadrupole-Orbitrap mass spectrometer (Thermo Scientific). The chromatography column consisted of a 50 cm long, 75 μm i.d. microcapillary capped by a 5 μm tip and packed with ReproSil-Pur 120 C18-AQ 2.4 μm beads (Dr. Maisch GmbH). LC solvents were 0.1 % FA in H2O (Buffer A) and 0.1 % FA in 90 % MeCN: 10 % H2O (Buffer B). Peptides were eluted into the mass spectrometer at a flow rate of 300 nL/min. over a 90 min linear gradient (5–35 % Buffer B) at 65 °C. Data was acquired in data-dependent mode (top-20, NCE 28, R = 15,000) after full MS scan (R = 60,000, m/z 400 – 1,300). Dynamic exclusion was set to 10 s, peptide match to prefer and isotope exclusion was enabled.
Bennett J.M., Narwal S.K., Kabeche S., Abegg D., Hackett F., Yeo T., Li V.L., Muir R.K., Faucher F.F., Lovell S., Blackman M.J., Adibekian A., Yeh E., Fidock D.A, & Bogyo M. (2024). Mixed Alkyl/Aryl Phosphonates Identify Metabolic Serine Hydrolases as Antimalarial Targets. bioRxiv.
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5

Nano-UHPLC-MS/MS Peptide Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peptides were resuspended in water with 0.1 % formic acid (FA) and analyzed using EASY-nLC 1200 nano-UHPLC coupled to a Q Exactive HF-X Quadrupole-Orbitrap mass spectrometer (Thermo Scientific). The chromatography column consisted of a 50 cm long, 75 μm i.d. microcapillary capped by a 5 μm tip and packed with ReproSil-Pur 120 C18-AQ 2.4 μm beads (Dr. Maisch GmbH). LC solvents were 0.1 % FA in H2O (Buffer A) and 0.1 % FA in 90 % MeCN: 10 % H2O (Buffer B). Peptides were eluted into the MS at a flow rate of 300 nL/min. over a 240 min. linear gradient (5-35 % Buffer B) at 65 °C. Data was acquired in data-dependent mode (top-20, NCE 28, R = 7,500) after full MS scan (R = 60,000, m/z 400-1,300). Dynamic exclusion was set to 10 s, peptide match to prefer and isotope exclusion was enabled.
Amatuni A., Shuster A., Adibekian A, & Renata H. (2020). Concise Chemoenzymatic Total Synthesis and Identification of Cellular Targets of Cepafungin I. Cell chemical biology, 27(10), 1318-1326.e18.
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