Dimethyl sulfoxide (DMSO) was purchased from Amresco (OH, USA), reverse transcription system kit and quantitative real-time PCR (qRT-PCR) kit from Toyobo Co. Ltd (Osaka, Japan), Phenol-free RPMI-1640 medium, DMEM, and F12K medium, fetal bovine serum (FBS), phosphate-buffered saline (PBS), penicillin/streptomycin, and 0.25% trypsin from Gibco Chemical. Co. (MA, USA), CCK-8 cell counting kit from TransGen Biotech Co. Ltd (Beijing, China), Gigantol from MCE (NJ, USA), TRIzol reagents from Invitrogen (Thermo Fisher Scientific, New Zealand). The human bladder cancer cell lines, SW780, 5637, and T24, and a normal urothelial cell line SVHUC-1 were acquired from Institute of Cell Biology, Chinese Academy of Science (Shanghai, China). UV spectra was recorded using an ELISA microplate reader (Bio-Rad Laboratories Inc., CA, USA). Images were taken using an inverted fluorescence microscope and camera system (Olympus Co., Tokyo, Japan). The Annexin V-PE/7-AAD apoptosis detection kit was provided by BD PharmingenTM (Shanghai, China). The antibodies against β-Actin, Axin2, Survivin, Slug, and Vimentin proteins were purchased from Affinity Biosciences (Melbourne, Australia). All other reagents used were of analytical grade and acquired from local vendors in China.
Cck 8 cell counting kit
Manufactured by Transgene
Sourced in United States, China
The CCK-8 Cell Counting Kit is a laboratory equipment used for the quantitative determination of viable cell numbers. It is a colorimetric assay that measures the dehydrogenase enzyme activity in living cells. The kit provides a simple, convenient, and sensitive method for measuring cell proliferation and cytotoxicity.
Automatically generated - may contain errors
Lab products found in correlation
Elisa microplate reader, by Bio-Rad (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
β actin, by Affinity Biosciences (1 mentions)
Annexin 5 pe 7 aad apoptosis detection kit, by BD (1 mentions)
Anti pparγ 16643 1 ap, by Proteintech (1 mentions)
3 protocols using cck 8 cell counting kit
1
Gigantol Inhibits Bladder Cancer Cell Growth
2
Cell Viability Assessed by CCK-8
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Cell viability was detected with the CCK-8 Cell Counting Kit (TransGen Biotech, FC101) in accordance with the manufacturer's instructions [38 (link)].
3
Molecular Mechanisms of Ferroptosis Regulation
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KA was purchased from Abcam (Cambridge, MA, USA). Deferoxamine (DFO) and RSL3 were obtained from TaoSu Biochemical Technology (Shanghai, China). Assay kits for iron, malondialdehyde (MDA) and GSH were purchased from Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China). CCK-8 Cell Counting Kit was purchased from TransGen Biotech (Beijing, China). ELISA kit for 4-hydroxynonenal (4-HNE) was obtained from Neobioscience (Shenzhen, China). Anti-CLOCK (ab3517) and anti-GAPDH (ab8245) antibodies were obtained from Abcam (Cambridge, MA). Anti-GPX4 (14432-1-AP) and anti-PPAR-γ (16643-1-AP) antibodies were purchased from Proteintech (Wuhan, China). Gpx4 luciferase reporters (-1974/+38 bp, -1315/+38 bp, -601/+38 bp and a mutated version), Ppar-γ luciferase reporters (-1900/+100 bp, -700/+100 bp, -29/+100 bp and a mutated version), pRL-TK, pcDNA3.1-Clock, pcDNA3.1, siClock (siRNA targeting Clock, sequence is shown in Table 1) and siNC (negative control for siRNA, sequence is shown in Table 1) were obtained from Transheep Technologies (Shanghai, China).
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