The largest database of trusted experimental protocols

Apc conjugated cd49d 9f10

Manufactured by BioLegend

APC-conjugated CD49d (9F10) is a flow cytometry reagent used for the detection and analysis of CD49d (very late antigen-4, VLA-4) expression on cells. CD49d is an integrin subunit that is involved in cell-cell and cell-extracellular matrix interactions.

Automatically generated - may contain errors

2 protocols using apc conjugated cd49d 9f10

1

Erythroid Cell Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry analysis, in vitro cultured erythroid cells were washed in FACS buffer (1% FBS in PBS) before antibody staining. The following antibodies at indicated dilutions were used in this study: 1:50 APC-conjugated CD235a (Glycophorin A, clone HIR2, 50–153-69, eBioscience), 1:50 FITC-conjugated CD71 (OKT9, 14–0719-82, eBioscience), 1:50 APC-conjugated CD49d (9F10, Biolegend, 304308) and 1:200 FITC-conjugated BAND3 (An et al., 2014 (link); Hu et al., 2013 (link)). For live/ dead cell discrimination Sytox Blue was used according to the manufacturer’s instructions (Thermo Fisher, S34857). For apoptosis analysis, BV421-Annexin V (BD Bioscience, 563973) was used in combination with Sytox Orange according to the manufacturer’s instructions (Thermo Fisher, S34861). FACS analysis was conducted on a BD Bioscience Fortessa flow cytometer at the Whitehead Institute Flow Cytometry core. Data were analyzed using FlowJo software v10.4.2.
+ Open protocol
+ Expand
2

Erythroid Cell Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry analysis, in vitro cultured erythroid cells were washed in FACS buffer (1% FBS in PBS) before antibody staining. The following antibodies at indicated dilutions were used in this study: 1:50 APC-conjugated CD235a (Glycophorin A, clone HIR2, 50–153-69, eBioscience), 1:50 FITC-conjugated CD71 (OKT9, 14–0719-82, eBioscience), 1:50 APC-conjugated CD49d (9F10, Biolegend, 304308) and 1:200 FITC-conjugated BAND3 (An et al., 2014 (link); Hu et al., 2013 (link)). For live/ dead cell discrimination Sytox Blue was used according to the manufacturer’s instructions (Thermo Fisher, S34857). For apoptosis analysis, BV421-Annexin V (BD Bioscience, 563973) was used in combination with Sytox Orange according to the manufacturer’s instructions (Thermo Fisher, S34861). FACS analysis was conducted on a BD Bioscience Fortessa flow cytometer at the Whitehead Institute Flow Cytometry core. Data were analyzed using FlowJo software v10.4.2.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!