Rabbit anti gfp a11122

Rabbit anti-β-Catenin (H102) (SC7199), rabbit GAPDH (SC25778) from Santa Cruz Biotechnology, and rabbit anti-GFP (A11122), mouse anti-GFP (A11120) from Invitrogen were all used at a 1:1000 dilution. Mouse anti-C-cadherin (5G5, hybridoma supernatant) and rabbit anti-C-cadherin, both generous gift of Dr Barry Gumbiner) were used, respectively, at 1:20 and 1:5000 dilutions^{32 (link)}.

The following antibodies, from the indicated sources, were used: rabbit anti-phospho-STAT3 (#9145), rabbit anti-STAT3 (#8768), rabbit anti-phospho-JAK1 (#74129), rabbit anti-myc (#9145), alpha-tubulin (DM1A, #3873) all from Cell Signaling Technology; mouse anti HA11 (#901514) and anti-Tuj1 (both from Biolegend); mouse anti-myc 9E10 (UPenn Cell Center); rabbit anti-GFP #A11122 (Invitrogen); mouse anti-JAK1 #610231 (BD Transduction Labs), mouse-anti NeuN #MAB377 (Millipore Sigma), Calnexin #10286 (Abcam). Unless otherwise noted, all chemicals were from ThermoFisher Biosciences and were of the highest reagent grade available.

Spinal columns of embryos and neonatal mice at the desired developmental stages were dissected out and directly immersed in PBS/4% paraformaldehyde (PFA) for 2 to 4 hr at 4°C. Postnatal mice were sacrificed with CO₂, transcardially perfused with 4% PFA, and spinal columns were dissected out and post-fixed with 4% PFA for 2 hr at 4°C. They were then cryo-protected in 1× PBS, 30% sucrose overnight. 20-µm frozen sections of SC and DRGs were cut using a CM1950 cryostat (Leica, Buffalo Grove, IL). Immunostaining of SCs and DRG sections were performed as described previously (Niu et al., 2013 (link)). Antibodies used are as follows: rabbit anti-GFP (A-11122, 1:2000, Invitrogen, Carlsbad, CA), chicken anti-GFP (GFP-1020, 1:1000, Aves, Tigard, OR), chicken anti-NF200 (NF-H, 1:500, Aves), rabbit anti-NF200 (N4142, 1:1000, Sigma, St. Louis, MO), rabbit anti-cRet (18121, 1:50, IBL, Minneapolis, MN), rabbit anti-NTRK1 (06-574, 1:1000, Millipore, Temecula, CA), guinea pig anti-VGLUT1 (AB5905, 1:1000, Millipore), rabbit anti-phospho-S6 (2215s, 1:200, Cell Signaling, Beverly, MA), and Alexa Fluorescent conjugated Goat or Donkey secondary antibodies (1:500, Invitrogen or Jackson ImmunoResearch, West Grove, PA).