Log In Sign up
The largest database of trusted experimental protocols

400 mesh carbon parlodion coated copper grid

Manufactured by Polysciences
Visit Supplier
Sourced in United Kingdom

The 400-mesh carbon parlodion-coated copper grid is a laboratory equipment designed for use in electron microscopy. It serves as a support for samples during electron microscope imaging. The grid is made of copper, coated with a thin layer of carbon and parlodion, and has a mesh size of 400 lines per inch.

Automatically generated - may contain errors

Lab products found in correlation

Ccd camera, by Advanced Microscopy Techniques (4 mentions) H 7600, by Hitachi (1 mentions)

4 protocols using 400 mesh carbon parlodion coated copper grid

1

Visualizing MERS-CoV and SARS-CoV S Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified MERS-CoV and SARS-CoV S proteins were adsorbed by flotation for 2 min onto a freshly discharged 400-mesh carbon parlodion-coated copper grid (Poly-Sciences, Warrington, PA). The grids were rinsed with buffer containing 20 mM Tris, pH 7.4, and 120 mM KCl and negatively stained with 1% phosphotungstic acid, then dried by aspiration. Virus-like particles were visualized on a Hitachi H-7600 transmission electron microscope (Hitachi High Technologies America, Schaumburg, IL) operating at 80 kV and digitally captured with a CCD camera at 1 K × 1 K resolution (Advanced Microscopy Techniques Corp., Danvers, MA).
Coleman C.M., Liu Y.V., Mu H., Taylor J.K., Massare M., Flyer D.C., Glenn G.M., Smith G.E, & Frieman M.B. (2014). Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice. Vaccine, 32(26), 3169-3174.
+ Open protocol
+ Expand
2

Visualizing MERS-CoV and SARS-CoV Spike Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified MERS-CoV and SARS-CoV S proteins were adsorbed by flotation for 2 minutes onto a freshly discharged 400-mesh carbon parlodion-coated copper grid (Poly-Sciences, Warrington, PA). The grids were rinsed with buffer containing 20mM Tris, pH 7.4, and 120mM KCl and negatively stained with 1% phosphotungstic acid, then dried by aspiration. Virus-like particles were visualized on a Hitachi H-7600 transmission electron microscope (Hitachi High Technologies America, Schaumburg, IL) operating at 80 kV and digitally captured with a CCD camera at 1K×1K resolution (Advanced Microscopy Techniques Corp., Danvers, MA).
Coleman C.M., Liu Y.V., Mu H., Taylor J.K., Massare M., Flyer D.C., Glenn G.M., Smith G.E, & Frieman M.B. (2014). Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice. Vaccine, 32(26), 3169-3174.
+ Open protocol
+ Expand
3

Visualizing Virus-Like Particles by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
VLP samples were adsorbed onto a freshly discharged 400 mesh carbon parlodion-coated copper grids (Poly-Sciences, Warrington, PA). The grids were rinsed with buffer containing 20 mM Tris, pH 7.4, and 120 mM KCl and negatively stained with 1% phosphotungstic acid, then dried by aspiration. The VLPs were visualized on a Hitachi H-7600 transmission electron microscope (Hitachi High Technologies America, Schaumburg, IL) operating at 80 kV and digitally captured with a CCD camera at 1k×1k resolution (Advanced Microscopy Techniques Corp., Danvers, MA).
Kapczynski D.R., Tumpey T.M., Hidajat R., Zsak A., Chrzastek K., Tretyakova I, & Pushko P. (2016). Vaccination with virus-like particles containing H5 antigens from three H5N1 clades protects chickens from H5N1 and H5N8 influenza viruses. Vaccine, 34(13), 1575-1581.
+ Open protocol
+ Expand
4

Visualizing Virus-Like Particles by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
VLP samples were adsorbed onto a freshly discharged 400 mesh carbon parlodion-coated copper grids (Poly-Sciences, Warrington, PA). The grids were rinsed with buffer containing 20 mM Tris, pH 7.4, and 120 mM KCl and negatively stained with 1% phosphotungstic acid, then dried by aspiration. VLPs were visualized on a Hitachi H-7600 transmission electron microscope (Hitachi High Technologies America, Schaumburg, IL) operating at 80 kV and digitally captured with a CCD camera at 1k×1k resolution (Advanced Microscopy Techniques Corp., Danvers, MA).
Tretyakova I., Hidajat R., Hamilton G., Horn N., Nickols B., Prather R.O., Tumpey T.M, & Pushko P. (2015). Preparation of Quadri-Subtype Influenza Virus-Like Particles Using Bovine Immunodeficiency Virus Gag Protein. Virology, 487, 163-171.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!